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anti-eIF2alpha antibody :: Rabbit eIF2alpha Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9407695
Unit / Price
0.05 mL  /  $200 +1 FREE 8GB USB
0.1 mL  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

eIF2alpha, Polyclonal Antibody

★Popular Item★
 Also Known As   

eIF2alpha (Phospho-Ser51) Antibody

 Product Synonym Names    eIF2a (Phospho-Ser51) Antibody; Eukaryotic translation initiation factor 2 subunit alpha; EIF-2A
 Product Gene Name   

anti-eIF2alpha antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    603907
 3D Structure    ModBase 3D Structure for P05198
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    The antibody detects endogenous level of eIF2a only when phosphorylated at serine 51.
 Purity/Purification    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
 Form/Format    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1.0 mg/ml (lot specific)
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 Immunogen Type    Peptide-KLH
 Immunogen Description    Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.
 Modification    Phospho-Ser51
 Target Name    eIF2a
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 Preparation and Storage    Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
 Other Notes    Small volumes of anti-eIF2alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-eIF2alpha antibody

   Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
 Product Categories/Family for anti-eIF2alpha antibody    Phospho-specific Ab
 Applications Tested/Suitable for anti-eIF2alpha antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-eIF2alpha antibody    Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
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 Western Blot (WB) of anti-eIF2alpha antibody    Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2a(Phospho-Ser51) Antibody.
anti-eIF2alpha antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-eIF2alpha antibody    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2alpha (Phospho-Ser51) Antibody (left) or the same antibody preincubated with blocking peptide #51279 (right).
anti-eIF2alpha antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-eIF2alpha antibody    Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(Phospho-Ser51) Antibody.
anti-eIF2alpha antibody Immunofluorescence (IF) image
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 Immunohistochemistry (IHC) of anti-eIF2alpha antibody    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2alpha (Phospho-Ser51) Antibody.
anti-eIF2alpha antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for eIF2alpha. It may not necessarily be applicable to this product.
 NCBI GI #    4758256
 NCBI GeneID    1965
 NCBI Accession #    NP_004085.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004094.4 [Other Products]
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 UniProt Primary Accession #    P05198 [Other Products]
 UniProt Related Accession #    P05198 [Other Products]
 Molecular Weight    36,112 Da
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 NCBI Official Full Name    eukaryotic translation initiation factor 2 subunit 1
 NCBI Official Synonym Full Names    eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
 NCBI Official Symbol    EIF2S1 [Similar Products]
 NCBI Official Synonym Symbols   
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
[Similar Products]
 NCBI Protein Information    eukaryotic translation initiation factor 2 subunit 1
 UniProt Protein Name    Eukaryotic translation initiation factor 2 subunit 1
 UniProt Synonym Protein Names   
Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
 UniProt Gene Name    EIF2S1 [Similar Products]
 UniProt Synonym Gene Names    EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha [Similar Products]
 UniProt Entry Name    IF2A_HUMAN
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 NCBI Summary for eIF2alpha    The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
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 UniProt Comments for eIF2alpha    eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: Translation initiation; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding

Biological Process: regulation of translation initiation in response to stress; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression
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 Research Articles on eIF2alpha    1. a single uORF is sufficient for eIF2-mediated translation control.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-eIF2alpha antibodyPathways associated with anti-eIF2alpha antibody
 Reference Product  PubMed Publications
 EIF2AK3 antibody  >4 publications with eIF2alpha and EIF2AK3
 EIF2AK2 antibody  >2 publications with eIF2alpha and EIF2AK2
 EIF2AK4 antibody  >2 publications with eIF2alpha and EIF2AK4
 EIF2S2 antibody  >2 publications with eIF2alpha and EIF2S2
 EIF5 antibody  >1 publications with eIF2alpha and EIF5
 EIF2S3 antibody  >1 publications with eIF2alpha and EIF2S3
 Products by Pathway  Pathway Diagram
 ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies  Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
Diseases associated with anti-eIF2alpha antibodyOrgans/Tissues associated with anti-eIF2alpha antibody
 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >1 publications with eIF2alpha and Cell Transformation, Neoplastic
 Cardiovascular Diseases Antibodies  >1 publications with eIF2alpha and Cardiovascular Diseases
 Hyperglycemia Antibodies  >1 publications with eIF2alpha and Hyperglycemia
 Glucose Intolerance Antibodies  >1 publications with eIF2alpha and Glucose Intolerance
 Disease Models, Animal Antibodies  >1 publications with eIF2alpha and Disease Models, Animal
 Inflammation Antibodies  >1 publications with eIF2alpha and Inflammation
 Necrosis Antibodies  >1 publications with eIF2alpha and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with eIF2alpha and Brain
 Heart Antibodies  >1 publications with eIF2alpha and Heart
 Muscle Antibodies  >1 publications with eIF2alpha and Muscle
 Pancreas Antibodies  >1 publications with eIF2alpha and Pancreas
 Blood Antibodies  >1 publications with eIF2alpha and Blood
 Placenta Antibodies  >1 publications with eIF2alpha and Placenta
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