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| Product Name
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egl nine homolog 3 (EGLN3), Polyclonal Antibody ★Popular Item★
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| Also Known As
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Rabbit anti-human egl nine homolog 3 (C. elegans) polyclonal Antibody
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| Product Synonym Names
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egl nine homolog 3 (C. elegans); EGLN3; FLJ21620; HIFPH3; MGC125998; MGC125999; PHD3
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| OMIM
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606426
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| Clonality
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Polyclonal
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| Isotype
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IgG
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| Host
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Rabbit
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| Species Reactivity
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Human, Mouse, Rat
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| Purity/Purification
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Antigen Affinity Purified
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| Immunogen
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Human EGLN3
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| Storage Buffer
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PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20 degree C, Avoid freeze / thaw cycles.
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| Santa Cruz Alternative
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Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46030 / sc-46031
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Other Notes
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Small volumes of anti-EGLN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for EGLN3 purchase
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Hypoxia effects is a good area of human research with relevance to cancer and neurodegeneration. Egl nine homology 3 EGLN 2 is an hypoxia inducible factor, in the HIF family. EGLN3 PHD3 is less well studied than HIF1, so it is an open avenue left to explore. To understand complex induction pathways and regulation, MyBiosource has teamed with Abgent to provide you with the top rabbit IgG polyclonal primary antibody for western blot, direct ELISA or IHC analysis. No other mouse mAb primary antibodies come close to our detection in cell lysates. This quality EGLN3 antibody detects human mouse protein equally and reacts to a wide range of secondary antibodies species. To learn more about our antibody services or to request quote for any of our anti HIF prolyl hydroxylase 3 products go to our website. You may win iPad mini by using promo code FLASH15.
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| Applications Tested/Suitable for anti-EGLN3 antibody
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ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
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| NCBI/Uniprot data below describe general gene information for EGLN3. It may not necessarily be applicable to this product. |
| NCBI GI #
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11545787
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| NCBI GeneID
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112399
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| NCBI Accession #
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NP_071356.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_022073.3
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[Other Products]
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| UniProt Secondary Accession #
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Q2TA79; Q3B8N4; Q6P1R2
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[Other Products]
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| UniProt Related Accession #
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Q9H6Z9
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[Other Products]
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| Molecular Weight
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27,261 Da
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[Similar Products]
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| NCBI Official Full Name
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egl nine homolog 3
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| NCBI Official Synonym Full Names
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egl-9 family hypoxia-inducible factor 3
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| NCBI Official Symbol
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EGLN3
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[Similar Products]
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| NCBI Official Synonym Symbols
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PHD3; HIFPH3; HIFP4H3
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[Similar Products]
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| NCBI Protein Information
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egl nine homolog 3; HPH-1; HPH-3; HIF-PH3; HIF prolyl hydroxylase 3; HIF-prolyl hydroxylase 3; egl nine-like protein 3 isoform; hypoxia-inducible factor prolyl hydroxylase 3; prolyl hydroxylase domain-containing protein 3
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| UniProt Protein Name
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Egl nine homolog 3
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| UniProt Synonym Protein Names
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HPH-1; Hypoxia-inducible factor prolyl hydroxylase 3; HIF-PH3; HIF-prolyl hydroxylase 3; HPH-3; Prolyl hydroxylase domain-containing protein 3; PHD3
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| Protein Family
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Egl nine
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| UniProt Gene Name
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EGLN3
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[Similar Products]
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| UniProt Synonym Gene Names
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HIF-PH3; HIF-prolyl hydroxylase 3; HPH-3; PHD3
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[Similar Products]
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| UniProt Entry Name
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EGLN3_HUMAN
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| UniProt Comments for EGLN3
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EGLN3: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation.
Protein type: Oxidoreductase; EC 1.14.11.29
Chromosomal Location of Human Ortholog: 14q13.1
Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity
Biological Process: caspase activation; apoptosis; regulation of neuron apoptosis; response to hypoxia; protein amino acid hydroxylation; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; response to DNA damage stimulus; regulation of cell proliferation
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| Research Articles on EGLN3
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1. PHD3 depletion did not affect the expression of the PDH-E1alpha, E1beta, and E2 subunits, or the phosphorylation status of E1alpha, but destabilized the PDH complex (PDC), resulting in less functional PDC.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-EGLN3 antibody | Pathways associated with anti-EGLN3 antibody |
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| Diseases associated with anti-EGLN3 antibody | Organs/Tissues associated with anti-EGLN3 antibody |
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Antibody 
