Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow mTOR rightarrow Polyclonal mTOR  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • fumarylacetoacetate hydrolase (fumarylacetoacetase) (FAH) ELISA Kit
 • FASL-TNFSF6 ELISA Kit
 • IL-17E - IL-25 Active Protein
 • Glutathione (GSH) Antibody
 • LAG-3 Antibody
 • Hepcidin 25 (Hepc25) ELISA Kit
 • CD14  ELISA Kit
 • Estrogen Receptor Beta (ERb) ELISA Kit
 • TFIIB Antibody
 • Galactose-1-phosphate uridylyltransferase (GALT) Recombinant Protein
 • Aquaporin 1 (AQP-1) ELISA Kit
 • 1-acyl-sn-glycerol-3-phosph ate acyltransferase epsilon (AGP... ELISA Kit
 • Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit
 • CMRF35 like molecule 8 (CD300A) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-mTOR antibody :: Rabbit anti-Human mTOR Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9406487
Unit / Price
0.05 mL  /  $200 +1 FREE 8GB USB
0.1 mL  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

mTOR, Polyclonal Antibody

★Popular Item★
 Also Known As   

mTOR (Phospho-Ser2448) Antibody

 Product Synonym Names    FRAP; FRAP1; FRAP2; RAFT1
 Product Gene Name   

anti-mTOR antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    601231
 3D Structure    ModBase 3D Structure for P42345
Section Bar
 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
Section Bar
 Specificity    The antibody detects endogenous level of mTOR only when phosphorylated at serine 2448.
 Purity/Purification    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
 Form/Format    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1.0 mg/ml (lot specific)
Section Bar
 Immunogen Type    Peptide-KLH
 Immunogen Description    Peptide sequence around phosphorylation site of serine 2448 (T-D-S(p)-Y-S) derived from Human mTOR.
 Modification    Phospho-Ser2448
 Target Name    mTOR
Section Bar
 Preparation and Storage    Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
 Other Notes    Small volumes of anti-mTOR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-mTOR antibody

   Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
 Product Categories/Family for anti-mTOR antibody    Phospho-specific Ab
 Applications Tested/Suitable for anti-mTOR antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-mTOR antibody    Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Section Bar
 Western Blot (WB) of anti-mTOR antibody    Western blot analysis of extracts from Hela cells, treatedwith IFNa or calf intestinal phosphatase (CIP), using mTOR(Phospho-Ser2448) Antibody.
anti-mTOR antibody Western Blot (WB) (WB) image
Section Bar
 Immunohistochemistry (IHC) of anti-mTOR antibody    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using mTOR(Phospho-Ser2448) Antibody (left) or the same antibody preincubated with blocking peptide(right).
anti-mTOR antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Immunofluorescence (IF) of anti-mTOR antibody    Immunofluorescence staining of methanol-fixed MCF cells using mTOR(Phospho-Ser2448) Antibody.
anti-mTOR antibody Immunofluorescence (IF) image
Section Bar
 Western Blot (WB) of anti-mTOR antibody    Western blot analysis of extracts from 293 cells untreatedor treated with PMA using mTOR(Phospho-Ser2448) Antibody.
anti-mTOR antibody Western Blot (WB) (WB) image
Section Bar
 Immunohistochemistry (IHC) of anti-mTOR antibody    Immunohistochemical analysis ofparaffin-embedded human breast carcinomatissue using mTOR (Phospho-Ser2448) Antibody
anti-mTOR antibody Immunohistochemistry (IHC) (IHC) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for mTOR. It may not necessarily be applicable to this product.
 NCBI GI #    4826730
 NCBI GeneID    2475
 NCBI Accession #    NP_004949.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004958.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P42345 [Other Products]
 UniProt Secondary Accession #    Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3 [Other Products]
 UniProt Related Accession #    P42345 [Other Products]
 Molecular Weight    288,892 Da
Table BarTOPTable Bar
 NCBI Official Full Name    serine/threonine-protein kinase mTOR
 NCBI Official Synonym Full Names    mechanistic target of rapamycin (serine/threonine kinase)
 NCBI Official Symbol    MTOR [Similar Products]
 NCBI Official Synonym Symbols   
FRAP; FRAP1; FRAP2; RAFT1; RAPT1
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase mTOR
 UniProt Protein Name    Serine/threonine-protein kinase mTOR
 UniProt Synonym Protein Names   
FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    MTOR [Similar Products]
 UniProt Synonym Gene Names    FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR [Similar Products]
 UniProt Entry Name    MTOR_HUMAN
Section Bar
 NCBI Summary for mTOR    The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
Section Bar
 UniProt Comments for mTOR    mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: EC 2.7.11.1; Autophagy; Motility/polarity/chemotaxis; Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; FRAP subfamily

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: PML body; endoplasmic reticulum membrane; lysosomal membrane; lysosome; dendrite; endomembrane system; cytosol; nucleoplasm; Golgi membrane; mitochondrial outer membrane; cell soma; membrane; cytoplasm; TORC2 complex; nucleus; phosphoinositide 3-kinase complex

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein domain specific binding; protein binding; phosphoprotein binding; ribosome binding; drug binding; kinase activity; ATP binding

Biological Process: negative regulation of autophagy; regulation of myelination; TOR signaling pathway; positive regulation of translation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; signal transduction; protein amino acid phosphorylation; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; protein catabolic process; cell growth; response to nutrient; regulation of response to food; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; peptidyl-threonine phosphorylation; positive regulation of lipid biosynthetic process; response to amino acid stimulus; negative regulation of NFAT protein import into nucleus; double-strand break repair via homologous recombination; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; ruffle organization and biogenesis; regulation of fatty acid beta-oxidation; positive regulation of transcription from RNA polymerase III promoter; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; regulation of protein kinase activity; vascular endothelial growth factor receptor signaling pathway; phosphorylation; growth
Table BarTOPTable Bar
 Research Articles on mTOR    1. Hyperphosphatemia induces endothelial autophagy, possibly through the inhibition of the Akt/mTOR signaling pathway, which may play a protective role against high Pi-induced apoptosis.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-mTOR antibodyPathways associated with anti-mTOR antibody
 Reference Product  PubMed Publications
 EIF4EBP1 antibody  >568 publications with mTOR and EIF4EBP1
 AKT1 antibody  >466 publications with mTOR and AKT1
 RICTOR antibody  >403 publications with mTOR and RICTOR
 RHEB antibody  >316 publications with mTOR and RHEB
 RPTOR antibody  >198 publications with mTOR and RPTOR
 MLST8 antibody  >49 publications with mTOR and MLST8
 RPS6KB1 antibody  >45 publications with mTOR and RPS6KB1
 AKT1S1 antibody  >33 publications with mTOR and AKT1S1
 MAPKAP1 antibody  >28 publications with mTOR and MAPKAP1
 FKBP1A antibody  >6 publications with mTOR and FKBP1A
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
Diseases associated with anti-mTOR antibodyOrgans/Tissues associated with anti-mTOR antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1285 publications with mTOR and Adenocarcinoma
 Kidney Diseases Antibodies  >1000 publications with mTOR and Kidney Diseases
 Disease Models, Animal Antibodies  >966 publications with mTOR and Disease Models, Animal
 Breast Neoplasms Antibodies  >874 publications with mTOR and Breast Neoplasms
 Carcinoma, Renal Cell Antibodies  >752 publications with mTOR and Carcinoma, Renal Cell
 Cardiovascular Diseases Antibodies  >696 publications with mTOR and Cardiovascular Diseases
 Inflammation Antibodies  >617 publications with mTOR and Inflammation
 Neoplasms, Experimental Antibodies  >590 publications with mTOR and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >580 publications with mTOR and Lung Neoplasms
 Carcinoma, Small Cell Antibodies  >491 publications with mTOR and Carcinoma, Small Cell
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2004 publications with mTOR and Blood
 Muscle Antibodies  >1601 publications with mTOR and Muscle
 Brain Antibodies  >1545 publications with mTOR and Brain
 Kidney Antibodies  >1538 publications with mTOR and Kidney
 Vascular Antibodies  >1435 publications with mTOR and Vascular
 Liver Antibodies  >1203 publications with mTOR and Liver
 Lung Antibodies  >1037 publications with mTOR and Lung
 Heart Antibodies  >700 publications with mTOR and Heart
 Bone Antibodies  >553 publications with mTOR and Bone
 Prostate Antibodies  >474 publications with mTOR and Prostate
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions