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anti-MAPK14 antibody :: Rabbit anti-Human p38 MAPK (Thr180/Tyr182) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502096
Testing Data
Unit / Price
0.1 mL  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

p38 MAPK (Thr180/Tyr182) (MAPK14), Polyclonal Antibody

 Also Known As   

Anti-Phospho-Thr180/Tyr182 p38 MAPK

 Product Gene Name   

anti-MAPK14 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 20; NC_005119.3 (7968528..8028708). Location: 20p12
 3D Structure    ModBase 3D Structure for P70618
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Specific for the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
 Purity/Purification    Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
 Form/Format    100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
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 Antigen    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr180 and phospho-Tyr182 of rat p38 MAPK.
 Immunogen Information    Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 conjugated to KLH
 Immunogen Species    Rat
 Reactivity Assumed Based on 100% Sequence Homology    Bovine, canine, chicken, mouse, non-human primates, rat and zebra fish
 Species Reactivity Note    The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, mouse, non-human primates, rat and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
 Biological Significance    The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002; Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182 (Lin et al., 1995).
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 Preparation and Storage    For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
 Other Notes    Small volumes of anti-MAPK14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-MAPK14 antibody

   Affinity purified rabbit polyclonal antibody
 Applications Tested/Suitable for anti-MAPK14 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-MAPK14 antibody    Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:250
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 Testing Data of anti-MAPK14 antibody    anti-MAPK14 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for MAPK14. It may not necessarily be applicable to this product.
 NCBI GI #    2499601
 NCBI GeneID    81649
 NCBI Accession #    P70618.3 [Other Products]
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 UniProt Primary Accession #    P70618 [Other Products]
 UniProt Secondary Accession #    O08594; Q99MG4 [Other Products]
 UniProt Related Accession #    P70618 [Other Products]
 Molecular Weight    39
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 NCBI Official Full Name    Mitogen-activated protein kinase 14
 NCBI Official Synonym Full Names    mitogen activated protein kinase 14
 NCBI Official Symbol    Mapk14 [Similar Products]
 NCBI Official Synonym Symbols   
RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
[Similar Products]
 NCBI Protein Information    mitogen-activated protein kinase 14; MAPK 14; MAP kinase 2; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; reactive kinase; MAP kinase p38 alpha; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; mitogen-activated protein kinase 14A; p38 mitogen activated protein kinase; mitogen-activated protein kinase p38 alpha; cytokine-supressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein
 UniProt Protein Name    Mitogen-activated protein kinase 14
 UniProt Synonym Protein Names   
CRK1; Mitogen-activated protein kinase p38 alpha
 UniProt Gene Name    Mapk14 [Similar Products]
 UniProt Synonym Gene Names    Csbp1; Csbp2; MAP kinase 14; MAPK 14 [Similar Products]
 UniProt Entry Name    MK14_RAT
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 NCBI Summary for MAPK14    mitogen-activated protein kinase; involved in intracellular signalling, inhibition of apoptosis and gene activation [RGD, Feb 2006]
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 UniProt Comments for MAPK14    Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3 STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Phosphorylates S100A9 at 'Thr-113'

By similarity.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

By similarity.

Enzyme regulation: Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as bacterial lipopolysaccharide (LPS) and interleukin-1. Activation occurs through dual phosphorylation of Thr-180 and Tyr-182 by either of two dual specificity kinases, MAP2K3/MKK3 or MAP2K6/MKK6, and potentially also MAP2K4/MKK4, as well as by TAB1-mediated autophosphorylation. MAPK14 phosphorylated on both Thr-180 and Tyr-182 is 10-20-fold more active than MAPK14 phosphorylated only on Thr-180, whereas MAPK14 phosphorylated on Tyr-182 alone is inactive. whereas Thr-180 is necessary for catalysis, Tyr-182 may be required for auto-activation and substrate recognition. Phosphorylated at Tyr-323 by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells. This alternative pathway is inhibited by GADD45A. Inhibited by dual specificity phosphatases, such as DUSP1, DUSP10, and DUSP16. Specifically inhibited by the binding of pyridinyl-imidazole compounds, which are cytokine-suppressive anti-inflammatory drugs (CSAID). SB203580 is an inhibitor of MAPK14

By similarity.

Subunit structure: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR

By similarity. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation

By similarity. Interacts with SPAG9 and GADD45A, CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1

By similarity. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity.

Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification: Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory cytokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16

By similarity.Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3

By similarity.Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.
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Product References and Citations for anti-MAPK14 antibody

   • Johnson GL, Lapadat R (2002) Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298:1911-1912.
• Lin A, Minden A, Martinetto H, Claret F-X, Lange-Carter C, Mercurio F, Johnson GL, Karin M (1995) Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2. Science 268:286-290.
• Philip S, Armstead WM (2003) Differential role of PTK, ERK and p38 MAPK in superoxide impairment of NMDA cerebrovasodilation. Brain Res 979:98-103.
• Shi Y, Gaestel M (2002) In the cellular garden of forking paths: How p38 MAPKs signal for downstream assistance. Biol Chem 383:1519-1536.
• Ying SW, Futter M, Rosenblum K, Webber MJ, Hunt SP, Bliss TVP, Bramham CR (2002) Brain-derived neurotrophic factor induces long-term potentiation in intact adult hippocampus: Requirement for ERK activation coupled to CREB and upregulation of Arc synthesis. J Neurosci 22:1532-1540.
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 Research Articles on MAPK14    1. The protection role of LXA4 against hypoxia/reoxygenation injury of cardiomyocytes is related to upregulation of HO-1, via activation of p38 MAPK pathway and nuclear translocation of Nrf2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-MAPK14 antibodyOrgans/Tissues associated with anti-MAPK14 antibody
 Products by Pathway  Pathway Diagram
 ADP Signalling Through P2Y Purinoceptor 1 Pathway antibodies  ADP Signalling Through P2Y Purinoceptor 1 Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies  Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 CDO In Myogenesis Pathway antibodies  CDO In Myogenesis Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >55 publications with MAPK14 and Muscle
 Heart Antibodies  >39 publications with MAPK14 and Heart
 Lung Antibodies  >25 publications with MAPK14 and Lung
 Liver Antibodies  >23 publications with MAPK14 and Liver
 Brain Antibodies  >22 publications with MAPK14 and Brain
 Embryonic Tissue Antibodies  >13 publications with MAPK14 and Embryonic Tissue
 Kidney Antibodies  >12 publications with MAPK14 and Kidney
 Ovary Antibodies  >10 publications with MAPK14 and Ovary
 Placenta Antibodies  >10 publications with MAPK14 and Placenta
 Connective Tissue Antibodies  >7 publications with MAPK14 and Connective Tissue
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