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| Product Name
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pyruvate dehydrogenase kinase, isozyme 2 (PDK2), Polyclonal Antibody
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| Also Known As
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Rabbit anti-human pyruvate dehydrogenase kinase, isozyme 2 polyclonal Antibody
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| Product Synonym Names
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pyruvate dehydrogenase kinase; isozyme 2; PDK2; PDHK2
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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602525
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| Clonality
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Polyclonal
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| Isotype
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IgG
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| Host
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Rabbit
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| Species Reactivity
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Human, Mouse, Rat
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| Purity/Purification
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Antigen Affinity Purified
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| Immunogen
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Human PDK2
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| Storage Buffer
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PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20 degree C, Avoid freeze / thaw cycles.
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| Santa Cruz Alternative
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Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-14484 / sc-14486
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Other Notes
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Small volumes of anti-PDK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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| Applications Tested/Suitable for anti-PDK2 antibody
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ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
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TOP
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| NCBI/Uniprot data below describe general gene information for PDK2. It may not necessarily be applicable to this product. |
| NCBI GI #
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38649337
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| NCBI GeneID
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5164
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| NCBI Accession #
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AAH63137.1
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[Other Products]
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| UniProt Secondary Accession #
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Q6P515; Q9BS05; A8K3A7; B3KNW0
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[Other Products]
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| UniProt Related Accession #
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Q15119
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[Other Products]
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| Molecular Weight
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38,864 Da
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[Similar Products]
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TOP
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| NCBI Official Full Name
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Pyruvate dehydrogenase kinase, isozyme 2
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| NCBI Official Synonym Full Names
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pyruvate dehydrogenase kinase, isozyme 2
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| NCBI Official Symbol
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PDK2
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[Similar Products]
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| NCBI Official Synonym Symbols
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PDHK2; PDKII
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[Similar Products]
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| NCBI Protein Information
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pyruvate dehydrogenase kinase, isozyme 2; PDH kinase 2; pyruvate dehydrogenase kinase, isoenzyme 2; pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
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| UniProt Protein Name
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[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
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| UniProt Synonym Protein Names
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Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII
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| UniProt Gene Name
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PDK2
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[Similar Products]
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| UniProt Synonym Gene Names
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PDHK2; PDH kinase 2; PDKII
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[Similar Products]
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| UniProt Entry Name
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PDK2_HUMAN
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| NCBI Summary for PDK2
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This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
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| UniProt Comments for PDK2
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PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.
Protein type: EC 2.7.11.2; Protein kinase, atypical; Mitochondrial; Kinase, protein; ATYPICAL group; PDHK family; PDHK subfamily
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial pyruvate dehydrogenase complex
Molecular Function: protein homodimerization activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity
Biological Process: cellular metabolic process; insulin receptor signaling pathway; glucose metabolic process; regulation of pH; regulation of gluconeogenesis; cellular response to nutrient; regulation of acetyl-CoA biosynthetic process from pyruvate; glucose homeostasis; pyruvate metabolic process; protein amino acid phosphorylation
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| Research Articles on PDK2
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1. The final compound of this series, 2-[(2,4-dihydroxyphenyl)sulfonyl]isoindoline-4,6-diol, designated PS10, inhibits all four PDK isoforms with IC50 = 0.8 muM for PDK2.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-PDK2 antibody | Pathways associated with anti-PDK2 antibody |
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| Diseases associated with anti-PDK2 antibody | Organs/Tissues associated with anti-PDK2 antibody |
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Antibody 
