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anti-Rho Kinase/ROCKII (pT396) antibody :: Rabbit Rho Kinase/ROCKII (pT396) Antibody

Scan QR to view Datasheet Catalog #    MBS462125
Testing Data
Unit / Price
0.1 mg  /  $285 +1 FREE 8GB USB
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 Product Name   

Rho Kinase/ROCKII (pT396) (Rho Kinase/ROCKII (pT396)), Antibody

★Popular Item★
 Also Known As   

Rabbit anti Phospho-ROCKII (pThr 396) antibody

 Product Synonym Names    Rabbit anti-ROCKII (pThr396); Rho Kinase; Rho-associated kinase-alpha (ROKalpha); Rho-associated; coiled-coil containing protein kinase II (ROCKII)
 Product Gene Name   

anti-Rho Kinase/ROCKII (pT396) antibody

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 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Chicken, Bovine, Canine
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 Specificity    This antibody recognizes ~165 kDa of human ROCKII at a phosphorylation site ofThr396. It does not react to non-phosphorylated protein. This antibody also reacts with mouse and rat. The other species are not tested.
 Purity/Purification    The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
 Form/Format    This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
 Concentration    Size: 100 ug/200 ul (lot specific)
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 Antigen Preparation    A synthetic peptide corresponding to the epitope -VETFP- with a single phosphorylation site Thr396 of human RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine.
 Positive Control    Conditioned 3T3 cell
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 Preparation and Storage    The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 20 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
 Other Notes    Small volumes of anti-Rho Kinase/ROCKII (pT396) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Rho Kinase/ROCKII (pT396) antibody

   Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
 Applications Tested/Suitable for anti-Rho Kinase/ROCKII (pT396) antibody   

ELISA, Western Blot, Immunohistochemistry

 Application Notes for anti-Rho Kinase/ROCKII (pT396) antibody    Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
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 Testing Data of anti-Rho Kinase/ROCKII (pT396) antibody    anti-Rho Kinase/ROCKII (pT396) antibody Testing Data image
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 Molecular Weight    165 kDa
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Product References and Citations for anti-Rho Kinase/ROCKII (pT396) antibody

   Susan Vahebi, et al, Functional Effects of Rho-Kinase-Dependent Phosphorylation of Specific Sites on Cardiac Troponin. Circulation Research. 2005;96:740-747.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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