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anti-Smad3 antibody :: Smad3 Antibody

Scan QR to view Datasheet Catalog #    MBS858478
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Smad3, Antibody

★Popular Item★
 Also Known As   

Smad3 (Phospho-Thr179) Antibody

 Product Synonym Names    JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3
 Product Gene Name   

anti-Smad3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    114500
 3D Structure    ModBase 3D Structure for P84022
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 Species Reactivity    Human, Mouse, Rat
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 Specificity    Smad3 (Phospho-Thr179) antibody detects endogenous levels of Smad3 only when phosphorylated at threonine 179.
 Purity/Purification    Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P).
 All Sites    Human: Thr179
Mouse: Thr179
Rat: Thr179
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 Preparation and Storage    Store at-20 degree C for 1 year.
 Other Notes    Small volumes of anti-Smad3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-Smad3 antibody    Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
 Applications Tested/Suitable for anti-Smad3 antibody   

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-Smad3 antibody    WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000
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 Testing Data of anti-Smad3 antibody    P-peptide-+
anti-Smad3 antibody Testing Data image
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 Immunohistochemistry (IHC) of anti-Smad3 antibody    Immunohistochemistry analysis of paraffin-embedded human thymus gland tissue using Smad3 (Phospho-Thr179) antibody. Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 2mins), using Smad3 (Phospho-Thr179) antibody.
anti-Smad3 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for Smad3. It may not necessarily be applicable to this product.
 NCBI GI #    223029440
 NCBI GeneID    4088
 NCBI Accession #    NP_001138574.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145102.1 [Other Products]
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 UniProt Primary Accession #    P84022 [Other Products]
 UniProt Secondary Accession #    O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383 [Other Products]
 UniProt Related Accession #    P84022 [Other Products]
 Molecular Weight    25,722 Da
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 NCBI Official Full Name    mothers against decapentaplegic homolog 3 isoform 2
 NCBI Official Synonym Full Names    SMAD family member 3
 NCBI Official Symbol    SMAD3 [Similar Products]
 NCBI Official Synonym Symbols   
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
[Similar Products]
 NCBI Protein Information    mothers against decapentaplegic homolog 3
 UniProt Protein Name    Mothers against decapentaplegic homolog 3
 UniProt Synonym Protein Names   
JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
 Protein Family    Mothers against decapentaplegic
 UniProt Gene Name    SMAD3 [Similar Products]
 UniProt Synonym Gene Names    MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3 [Similar Products]
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 NCBI Summary for Smad3    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
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 UniProt Comments for Smad3    Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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Product References and Citations for anti-Smad3 antibody

   Zhang Y., Nature 383:168-172(1996). Riggins G.J., Nat. Genet. 13:347-349(1996). Arai T., Cancer Lett. 122:157-163(1998).
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 Research Articles on Smad3    1. ERK1/2 mediates Heme oxygenase-1 or CO-induced Smad3 phosphorylation at Thr179.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Smad3 antibodyPathways associated with anti-Smad3 antibody
 Reference Product  PubMed Publications
 SMAD2 antibody  >1384 publications with Smad3 and SMAD2
 SMAD4 antibody  >729 publications with Smad3 and SMAD4
 JUN antibody  >407 publications with Smad3 and JUN
 SMAD1 antibody  >169 publications with Smad3 and SMAD1
 MYC antibody  >88 publications with Smad3 and MYC
 CTNNB1 antibody  >85 publications with Smad3 and CTNNB1
 SP1 antibody  >72 publications with Smad3 and SP1
 SKI antibody  >63 publications with Smad3 and SKI
 MAPK1 antibody  >44 publications with Smad3 and MAPK1
 RUNX2 antibody  >37 publications with Smad3 and RUNX2
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
Diseases associated with anti-Smad3 antibodyOrgans/Tissues associated with anti-Smad3 antibody
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >674 publications with Smad3 and Fibrosis
 Neoplasms Antibodies  >581 publications with Smad3 and Neoplasms
 Disease Models, Animal Antibodies  >245 publications with Smad3 and Disease Models, Animal
 Inflammation Antibodies  >244 publications with Smad3 and Inflammation
 Liver Diseases Antibodies  >175 publications with Smad3 and Liver Diseases
 Kidney Diseases Antibodies  >152 publications with Smad3 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >145 publications with Smad3 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >100 publications with Smad3 and Breast Neoplasms
 Necrosis Antibodies  >92 publications with Smad3 and Necrosis
 Liver Cirrhosis Antibodies  >91 publications with Smad3 and Liver Cirrhosis
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >452 publications with Smad3 and Muscle
 Lung Antibodies  >276 publications with Smad3 and Lung
 Skin Antibodies  >195 publications with Smad3 and Skin
 Heart Antibodies  >143 publications with Smad3 and Heart
 Embryonic Tissue Antibodies  >137 publications with Smad3 and Embryonic Tissue
 Intestine Antibodies  >56 publications with Smad3 and Intestine
 Thyroid Antibodies  >28 publications with Smad3 and Thyroid
 Ascites Antibodies  >1 publications with Smad3 and Ascites
 Uncharacterized Tissue Antibodies  >1 publications with Smad3 and Uncharacterized Tissue
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