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anti-TIE2 antibody :: Rabbit anti-Human, mouse TIE2 (Phospho-Tyr1108) Antibody

Scan QR to view Datasheet Catalog #    MBS003398
Western Blot (WB)
Unit / Price
0.05 mL  /  $270 +1 FREE 8GB USB
0.1 mL  /  $445 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TIE2 (Phospho-Tyr1108), Antibody

★Popular Item★
 Also Known As   

Rabbit TIE2 (Phospho-Tyr1108) Antibody

 Product Synonym Names    Angiopoietin 1 receptor; Angiopoietin-1 receptor; CD202b; CD202b antigen; Endothelialtyrosine kinase; Endothelium specific receptor tyrosine kinase 2; hTIE 2; hTIE2; Hyk; p140TEK; Soluble TIE2 variant 1; Soluble TIE2 variant 2; Tek; tek tyrosine kinase; TEK tyrosine kinase endothelial; tek tyrosine kinase, endothelial; TIE 2; TIE2; TIE2_HUMAN; Tunicainterna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains 2;Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine protein kinase receptor TEK; Tyrosine protein kinase receptor TIE 2; Tyrosine-protein kinase receptor TEK;Tyrosine-protein kinase receptor TIE-2; Venous malformations multiple cutaneous and mucosal; VMCM 1; VMCM; VMCM1;
 Product Gene Name   

anti-TIE2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 9; NC_000009.11 (27109147..27230172). Location: 9p21
 OMIM    600195
 3D Structure    ModBase 3D Structure for Q02763
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 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, mouse
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 Specificity    Phospho-Tie2 (Tyr1108) Antibody detects endogenous levels of Tie2.
 Purity/Purification    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
 Form/Format    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
 Concentration    1 mg/ml (lot specific)
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 Buffer    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Immunogen    A synthesized peptide derived from human Tie2 around the phosphorylation site of Tyr1108
 Function    Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.
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 Preparation and Storage    Store at -20C.Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-TIE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-TIE2 antibody   

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

 Application Notes for anti-TIE2 antibody    WB 1:500-1:2000
IHC 1:50-1:200
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 Western Blot (WB) of anti-TIE2 antibody    Western blot analysis of extracts of 293 cells transfected with wild-type Tie2, using Phospho-Tie2 (Tyr1108) Antibody.
anti-TIE2 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for TIE2. It may not necessarily be applicable to this product.
 NCBI GI #    88758596
 NCBI GeneID    7010
 NCBI Accession #    NP_000450.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000459.3 [Other Products]
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 UniProt Primary Accession #    Q02763 [Other Products]
 UniProt Secondary Accession #    Q5TCU2; Q8IV34; A8K6W0; B4DH20; B4DHD3; D3DRK5; E7EWI2 [Other Products]
 UniProt Related Accession #    Q02763 [Other Products]
 Molecular Weight    126 Kda
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 NCBI Official Full Name    angiopoietin-1 receptor
 NCBI Official Synonym Full Names    TEK tyrosine kinase, endothelial
 NCBI Official Symbol    TEK [Similar Products]
 NCBI Official Synonym Symbols   
TIE2; VMCM; TIE-2; VMCM1; CD202B
[Similar Products]
 NCBI Protein Information    angiopoietin-1 receptor; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine kinase with Ig and EGF homology domains-2
 UniProt Protein Name    Angiopoietin-1 receptor
 UniProt Synonym Protein Names   
Endothelial tyrosine kinase; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; hTIE2; p140 TEK
 Protein Family    Tyrosine-protein kinase receptor
 UniProt Gene Name    TEK [Similar Products]
 UniProt Synonym Gene Names    TIE2; VMCM; VMCM1; hTIE2 [Similar Products]
 UniProt Entry Name    TIE2_HUMAN
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 NCBI Summary for TIE2    The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TIE2    TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: EC 2.7.10.1; Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; Tie family

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: cell surface; focal adhesion; microvillus; basolateral plasma membrane; integral to plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; basal plasma membrane; stress fiber; plasma membrane

Molecular Function: protein binding; growth factor binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; receptor activity; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; peptidyl-tyrosine phosphorylation; response to cAMP; heart development; protein amino acid autophosphorylation; cell-matrix adhesion; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; positive regulation of focal adhesion formation; cell-cell signaling; angiogenesis; endochondral ossification; Tie receptor signaling pathway; organ regeneration; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; negative regulation of inflammatory response; endothelial cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; regulation of vascular permeability; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Venous Malformations, Multiple Cutaneous And Mucosal
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 Research Articles on TIE2    1. This work reveals an anti-angiogenic role of STAT1 activation that acts in Tie2-R849W-expressing endothelial cells to impair VEGF-A-mediated STAT3 signaling, bFGF production, and smooth muscle cell recruitment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-TIE2 antibodyPathways associated with anti-TIE2 antibody
 Reference Product  PubMed Publications
 ANGPT1 antibody  >62 publications with TIE2 and ANGPT1
 TIE1 antibody  >13 publications with TIE2 and TIE1
 GRB7 antibody  >3 publications with TIE2 and GRB7
 GRB14 antibody  >3 publications with TIE2 and GRB14
 DOK2 antibody  >3 publications with TIE2 and DOK2
 PTPN11 antibody  >2 publications with TIE2 and PTPN11
 ANGPTL1 antibody  >2 publications with TIE2 and ANGPTL1
 SHC1 antibody  >1 publications with TIE2 and SHC1
 ANGPT4 antibody  >1 publications with TIE2 and ANGPT4
 Products by Pathway  Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram
 Tie2 Signaling Pathway antibodies  Tie2 Signaling Pathway Diagram
Diseases associated with anti-TIE2 antibodyOrgans/Tissues associated with anti-TIE2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >95 publications with TIE2 and Neoplasms
 Cardiovascular Diseases Antibodies  >45 publications with TIE2 and Cardiovascular Diseases
 Neovascularization, Pathologic Antibodies  >44 publications with TIE2 and Neovascularization, Pathologic
 Inflammation Antibodies  >31 publications with TIE2 and Inflammation
 Neoplasms, Experimental Antibodies  >21 publications with TIE2 and Neoplasms, Experimental
 Brain Diseases Antibodies  >16 publications with TIE2 and Brain Diseases
 Heart Diseases Antibodies  >16 publications with TIE2 and Heart Diseases
 Lung Neoplasms Antibodies  >12 publications with TIE2 and Lung Neoplasms
 Liver Diseases Antibodies  >12 publications with TIE2 and Liver Diseases
 Hypertension Antibodies  >11 publications with TIE2 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >169 publications with TIE2 and Vascular
 Embryonic Tissue Antibodies  >49 publications with TIE2 and Embryonic Tissue
 Muscle Antibodies  >46 publications with TIE2 and Muscle
 Lung Antibodies  >43 publications with TIE2 and Lung
 Brain Antibodies  >29 publications with TIE2 and Brain
 Kidney Antibodies  >22 publications with TIE2 and Kidney
 Eye Antibodies  >8 publications with TIE2 and Eye
 Placenta Antibodies  >8 publications with TIE2 and Placenta
 Connective Tissue Antibodies  >7 publications with TIE2 and Connective Tissue
 Uterus Antibodies  >7 publications with TIE2 and Uterus
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