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TNF antibody :: TNF-alpha (Tumor Necrosis Factor alpha) Antibody

Scan QR to view Datasheet Catalog #    MBS438099
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $150 +1 FREE 8GB USB
7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $270 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $285 +1 FREE 8GB USB
0.2 mg (With BSA & Azide at 0.2mg/ml)  /  $360 +1 FREE 8GB USB
0.2 mg (Without BSA & Azide at 1mg/ml)  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TNF-alpha (Tumor Necrosis Factor alpha), Antibody

 Also Known As   

TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody

 Product Synonym Names    APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor
 Product Gene Name   

TNF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    191160
 3D Structure    ModBase 3D Structure for P01375
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 Clonality    Monoclonal
 Isotype    IgM, kappa
 Clone Number    TNF706
 Host    Mouse
 Species Reactivity    Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish
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 Specificity    Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
 Form/Format    Clear, colorless to yellowish liquid, 0.2 um filtered
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 Cellular Localization    Cytoplasmic and extracellular (Secreted)
 Immunogen    Recombinant N-terminal fragment of human TNF-alpha
 Hu-Chromosome Location    6p21.33
 Positive Control    HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
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 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of TNF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for TNF antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin

 Application Notes for TNF antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-paraffin) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
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 Immunohistochemistry (IHC) of TNF antibody    Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNF706).
TNF antibody  Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of TNF antibody    Formalin-fixed, paraffin-embedded Rat Pancreas stained with TNF alpha Monoclonal Antibody (TNF706)
TNF antibody  Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.
 NCBI GI #    25952111
 NCBI GeneID    7124
 NCBI Accession #    NP_000585.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000594.3 [Other Products]
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 UniProt Primary Accession #    P01375 [Other Products]
 UniProt Secondary Accession #    O43647; Q9P1Q2; Q9UIV3 [Other Products]
 UniProt Related Accession #    P01375 [Other Products]
 Molecular Weight    17kDa
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 NCBI Official Full Name    tumor necrosis factor
 NCBI Official Synonym Full Names    tumor necrosis factor
 NCBI Official Symbol    TNF [Similar Products]
 NCBI Official Synonym Symbols   
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
[Similar Products]
 NCBI Protein Information    tumor necrosis factor
 UniProt Protein Name    Tumor necrosis factor
 UniProt Synonym Protein Names   
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
 Protein Family    Tumor necrosis factor
 UniProt Gene Name    TNF [Similar Products]
 UniProt Synonym Gene Names    TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2 [Similar Products]
 UniProt Entry Name    TNFA_HUMAN
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 NCBI Summary for TNF    This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TNF    TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; external side of plasma membrane; extracellular region; extracellular space; integral to plasma membrane; lipid raft; phagocytic cup; plasma membrane; recycling endosome

Molecular Function: cytokine activity; identical protein binding; protease binding; protein binding; tumor necrosis factor receptor binding

Biological Process: activation of MAPK activity; activation of MAPKKK activity; activation of NF-kappaB transcription factor; caspase activation; chronic inflammatory response to antigenic stimulus; cortical actin cytoskeleton organization and biogenesis; embryonic gut development; I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; inflammatory response; leukocyte tethering or rolling; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; negative regulation of cytokine secretion during immune response; negative regulation of interleukin-6 production; negative regulation of lipid catabolic process; negative regulation of protein complex disassembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell adhesion; positive regulation of chemokine biosynthetic process; positive regulation of chemokine production; positive regulation of cytokine production; positive regulation of cytokine secretion; positive regulation of fever; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of membrane protein ectodomain proteolysis; positive regulation of NF-kappaB import into nucleus; positive regulation of NFAT protein import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phagocytosis; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein complex disassembly; positive regulation of protein kinase activity; positive regulation of protein transport; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein import into nucleus, translocation; protein kinase B signaling cascade; receptor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; response to glucocorticoid stimulus; response to salt stress; response to virus; sequestering of triacylglycerol; tumor necrosis factor-mediated signaling pathway

Disease: Asthma, Susceptibility To; Malaria, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1
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Product References and Citations for TNF antibody

   McLaughlin PJ; Elwood NJ; Ramadi LT, Pica MR, McKenzie IF. Improvement in sensitivity of enzyme-linked immunosorbent assay for tumor necrosis factor. Immunol Cell Biol, 1990, 68:51-5.
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 Research Articles on TNF    1. TNFalpha-308 A allele and A/A genotype were positively associated to erosive RA. G allele and GG genotype of the TNFalpha-308 were protectors.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TNF antibody Pathways associated with TNF antibody
 Reference Product  PubMed Publications
 ICAM1 antibody  >3666 publications with TNF and ICAM1
 FOXP3 antibody  >819 publications with TNF and FOXP3
 CD83 antibody  >575 publications with TNF and CD83
 TNFRSF1A antibody  >505 publications with TNF and TNFRSF1A
 MYC antibody  >465 publications with TNF and MYC
 MMP9 antibody  >379 publications with TNF and MMP9
 IFNG antibody  >235 publications with TNF and IFNG
 RELB antibody  >179 publications with TNF and RELB
 IKBKG antibody  >75 publications with TNF and IKBKG
 ITGAM antibody  >49 publications with TNF and ITGAM
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
Diseases associated with TNF antibody Organs/Tissues associated with TNF antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >99436 publications with TNF and Necrosis
 Inflammation Antibodies  >38384 publications with TNF and Inflammation
 Lung Diseases Antibodies  >7637 publications with TNF and Lung Diseases
 Liver Diseases Antibodies  >5863 publications with TNF and Liver Diseases
 Colitis Antibodies  >2901 publications with TNF and Colitis
 Insulin Resistance Antibodies  >2893 publications with TNF and Insulin Resistance
 Fibrosis Antibodies  >2874 publications with TNF and Fibrosis
 Atherosclerosis Antibodies  >2730 publications with TNF and Atherosclerosis
 Pain Antibodies  >2669 publications with TNF and Pain
 Reperfusion Injury Antibodies  >2470 publications with TNF and Reperfusion Injury
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >56629 publications with TNF and Blood
 Lung Antibodies  >10781 publications with TNF and Lung
 Brain Antibodies  >8155 publications with TNF and Brain
 Heart Antibodies  >5702 publications with TNF and Heart
 Intestine Antibodies  >4196 publications with TNF and Intestine
 Connective Tissue Antibodies  >2568 publications with TNF and Connective Tissue
 Lymph Node Antibodies  >2109 publications with TNF and Lymph Node
 Placenta Antibodies  >938 publications with TNF and Placenta
 Prostate Antibodies  >900 publications with TNF and Prostate
 Ovary Antibodies  >603 publications with TNF and Ovary
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