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anti-VIME antibody :: anti-Human Vimentin Antibody

Scan QR to view Datasheet Catalog #    MBS859600
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Vimentin, Antibody

★Popular Item★
 Also Known As   

Vimentin (Phospho-Ser56) Antibody

 Product Synonym Names    vimentin
 Product Gene Name   

anti-VIME antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    116300
 3D Structure    ModBase 3D Structure for P08670
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 Species Reactivity    Human
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 Specificity    Vimentin (Phospho-Ser56) antibody detects endogenous levels of Vimentin only when phosphorylated at serine 56.
 Purity/Purification    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 56 (A-S-SP-P-G).
 All Sites    Human: Ser56
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 Preparation and Storage    Store at-20 degree C for 1 year.
 Other Notes    Small volumes of anti-VIME antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VIME purchase    Vimentin (or Vim) is a type III intermediate filament (IF) protein that makes up the bulk of the cytoskeleton in mesenchymal cells. The quaternary structure of vimentin is a coiled-coil dimer of two identical monomers. The functions of vimentin include supporting and anchoring various organelles within the cytoplasm, (nucleus, mitochondria, and the endoplasmic reticulum), preserving the cell shape and integrity of the cytoplasm, and stabilizing the interaction of other cytoskeletal components. Additionally, vimentin controls the transportation of LDL-derived cholesterol from lysosomes to its esterification site.
Vimentin has recently been found to be overexpressed in several epithelial cancers such as breast, lunch, prostate, and malignant melanomas. This correlates with increased tumor growth, metastasis, and poor prognosis. These factors make vimentin a potential target for cancer treatments.
During mitosis, vimentin is phosphorylated at Ser56 by cyclin dependent kinase 1 (cdk1). This phosphorylation provides a binding site for Polo-like kinase (PLK), a mediator of the G2/M cell cycle transition.
This rabbit IgG polyclonal anti vimentin (phosphor-Ser56) antibody only recognizes the human vimentin when phosphorylated at serine 56. It is suitable for western blot, immunohistochemistry of fixed tissue, and ELISA applications.
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 Product Categories/Family for anti-VIME antibody    Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
 Applications Tested/Suitable for anti-VIME antibody   

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-VIME antibody    WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
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 Testing Data of anti-VIME antibody    P-peptide-+
anti-VIME antibody Testing Data image
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 Immunohistochemistry (IHC) of anti-VIME antibody    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vimentin (Phospho-Ser56) antibody. Western blot analysis of extracts from A549 cells, treated with Nocodazole (1ug/ml, 16hours), using Vimentin (Phospho-Ser56) antibody.
anti-VIME antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for VIME. It may not necessarily be applicable to this product.
 NCBI GI #    62414289
 NCBI GeneID    7431
 NCBI Accession #    NP_003371.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003380.4 [Other Products]
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 UniProt Primary Accession #    P08670 [Other Products]
 UniProt Secondary Accession #    Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4 [Other Products]
 UniProt Related Accession #    P08670 [Other Products]
 Molecular Weight    53,652 Da
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 NCBI Official Full Name    vimentin
 NCBI Official Synonym Full Names    vimentin
 NCBI Official Symbol    VIM [Similar Products]
 NCBI Protein Information    vimentin
 UniProt Protein Name    Vimentin
 Protein Family    Vimentin
 UniProt Gene Name    VIM [Similar Products]
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 NCBI Summary for VIME    This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
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 UniProt Comments for VIME    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
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Product References and Citations for anti-VIME antibody

   Ota T., Nat. Genet. 36:40-45(2004). Rasmussen R.K., Electrophoresis 18:588-598(1997). Gupta A.K., Gene 86:303-304(1990).
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 Research Articles on VIME    1. our results demonstrated that vimentin in human GC tissues and cell lines was upregulated due to its de-ubiquitination after interactions with USP14 and miR-320a, which could promote the aggressiveness of GC cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with anti-VIME antibodyOrgans/Tissues associated with anti-VIME antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >383 publications with VIME and Nervous System Diseases
 Neoplasms Antibodies  >283 publications with VIME and Neoplasms
 Brain Diseases Antibodies  >246 publications with VIME and Brain Diseases
 Neurodegenerative Diseases Antibodies  >166 publications with VIME and Neurodegenerative Diseases
 Inflammation Antibodies  >46 publications with VIME and Inflammation
 Kidney Diseases Antibodies  >37 publications with VIME and Kidney Diseases
 Hemorrhage Antibodies  >32 publications with VIME and Hemorrhage
 Disease Models, Animal Antibodies  >32 publications with VIME and Disease Models, Animal
 Pain Antibodies  >31 publications with VIME and Pain
 Liver Diseases Antibodies  >29 publications with VIME and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >461 publications with VIME and Brain
 Blood Antibodies  >187 publications with VIME and Blood
 Muscle Antibodies  >101 publications with VIME and Muscle
 Nerve Antibodies  >79 publications with VIME and Nerve
 Bone Antibodies  >69 publications with VIME and Bone
 Kidney Antibodies  >52 publications with VIME and Kidney
 Liver Antibodies  >51 publications with VIME and Liver
 Bone Marrow Antibodies  >46 publications with VIME and Bone Marrow
 Eye Antibodies  >38 publications with VIME and Eye
 Vascular Antibodies  >35 publications with VIME and Vascular
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