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CDKN1A antibody :: p21WAF1 Antibody

Scan QR to view Datasheet Catalog #    MBS438032
Immunohistochemistry (IHC)
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

p21WAF1 (CDKN1A), Antibody

★Popular Item★
 Also Known As   

p21WAF1 (Tumor Suppressor Protein) Mouse Monoclonal Antibody

 Product Synonym Names    Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, p21Cip1/Waf1, PIC1, SDI1, SLC12A9, Wild type p53 activated fragment 1 (WAF1)
 Product Gene Name   

CDKN1A antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    116899
 3D Structure    ModBase 3D Structure for P38936
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 Clonality    Monoclonal
 Isotype    IgG1, kappa
 Clone Number    WA-1
 Host    Mouse
 Species Reactivity    Human, Chimpanzee, Monkey, Mouse, Rat
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 Specificity    This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Cellular Localization    Nuclear
 Immunogen    Human recombinant p21 protein
 Hu-Chromosome Location    6p21.31
 Positive Control    HeLa Cells. Skin, colon, or breast carcinoma.
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 Preparation and Storage    Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of CDKN1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for CDKN1A antibody   

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

 Application Notes for CDKN1A antibody    Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
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 Immunohistochemistry (IHC) of CDKN1A antibody    Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Monoclonal Antibody (WA-1).
CDKN1A antibody  Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CDKN1A. It may not necessarily be applicable to this product.
 NCBI GI #    11386203
 NCBI GeneID    1026
 NCBI Accession #    NP_000380.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000389.4 [Other Products]
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 UniProt Primary Accession #    P38936 [Other Products]
 UniProt Secondary Accession #    Q14010; Q6FI05; Q9BUT4 [Other Products]
 UniProt Related Accession #    P38936 [Other Products]
 Molecular Weight    21kDa
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 NCBI Official Full Name    cyclin-dependent kinase inhibitor 1 isoform 1
 NCBI Official Synonym Full Names    cyclin dependent kinase inhibitor 1A
 NCBI Official Symbol    CDKN1A [Similar Products]
 NCBI Official Synonym Symbols   
P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
[Similar Products]
 NCBI Protein Information    cyclin-dependent kinase inhibitor 1
 UniProt Protein Name    Cyclin-dependent kinase inhibitor 1
 UniProt Synonym Protein Names   
CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
 UniProt Gene Name    CDKN1A [Similar Products]
 UniProt Synonym Gene Names    CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6 [Similar Products]
 UniProt Entry Name    CDN1A_HUMAN
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 NCBI Summary for CDKN1A    This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]
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 UniProt Comments for CDKN1A    p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Cell cycle regulation; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase activating kinase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; ubiquitin protein ligase binding

Biological Process: cell cycle arrest; cellular response to amino acid starvation; cellular response to extracellular stimulus; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of phosphorylation; positive regulation of fibroblast proliferation; protein stabilization; Ras protein signal transduction; regulation of cyclin-dependent protein kinase activity; response to DNA damage stimulus
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Product References and Citations for CDKN1A antibody

   Kovaric, J. et al., Int.J.Oncol. 9(suppl.), 835 (1996).
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 Research Articles on CDKN1A    1. It was concluded that over-activation of the mTORC1/4E-BP1/p21 pathway is a frequent and clinically relevant alteration in head and neck squamous cell carcinomas.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDKN1A antibody Pathways associated with CDKN1A antibody
 Reference Product  PubMed Publications
 CDK2 antibody  >1289 publications with CDKN1A and CDK2
 CDK4 antibody  >830 publications with CDKN1A and CDK4
 TP53 antibody  >794 publications with CDKN1A and TP53
 PCNA antibody  >778 publications with CDKN1A and PCNA
 CCNB1 antibody  >355 publications with CDKN1A and CCNB1
 CCND1 antibody  >222 publications with CDKN1A and CCND1
 SKP2 antibody  >116 publications with CDKN1A and SKP2
 CCND3 antibody  >84 publications with CDKN1A and CCND3
 CCND2 antibody  >66 publications with CDKN1A and CCND2
 CCNE1 antibody  >44 publications with CDKN1A and CCNE1
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 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
Diseases associated with CDKN1A antibody Organs/Tissues associated with CDKN1A antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1046 publications with CDKN1A and Adenocarcinoma
 Breast Neoplasms Antibodies  >890 publications with CDKN1A and Breast Neoplasms
 Intestinal Neoplasms Antibodies  >699 publications with CDKN1A and Intestinal Neoplasms
 Lung Neoplasms Antibodies  >499 publications with CDKN1A and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >492 publications with CDKN1A and Neoplasms, Experimental
 Liver Diseases Antibodies  >467 publications with CDKN1A and Liver Diseases
 Liver Neoplasms Antibodies  >428 publications with CDKN1A and Liver Neoplasms
 Prostatic Neoplasms Antibodies  >407 publications with CDKN1A and Prostatic Neoplasms
 Colonic Neoplasms Antibodies  >389 publications with CDKN1A and Colonic Neoplasms
 Carcinoma, Hepatocellular Antibodies  >374 publications with CDKN1A and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >978 publications with CDKN1A and Blood
 Lung Antibodies  >843 publications with CDKN1A and Lung
 Muscle Antibodies  >753 publications with CDKN1A and Muscle
 Liver Antibodies  >711 publications with CDKN1A and Liver
 Vascular Antibodies  >621 publications with CDKN1A and Vascular
 Prostate Antibodies  >499 publications with CDKN1A and Prostate
 Bone Antibodies  >440 publications with CDKN1A and Bone
 Skin Antibodies  >436 publications with CDKN1A and Skin
 Brain Antibodies  >400 publications with CDKN1A and Brain
 Kidney Antibodies  >363 publications with CDKN1A and Kidney
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