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96-well Cellular Senescence Assay (SA beta-Gal Activity) Assay Kit

Scan QR to view Datasheet Catalog #    MBS168468
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24 Assays  /  $325 +1 FREE 8GB USB
120 Assays  /  $595 +1 FREE 8GB USB
600 Assays  /  $2,385 +2 FREE 8GB USB
 
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 Product Name   

96-well Cellular Senescence Assay (SA beta-Gal Activity), Assay Kit

 Also Known As   

96-well Cellular Senescence Assay (SA beta-Gal Activity)

 Product Synonym Names    96-well Cellular Senescence Assay (SA beta-Gal Activity); 96-well Cellular Senescence Assay (SA b-Gal Activity)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
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 Preparation and Storage    Store SA-beta-gal substrate solution protected from light at -20 degree C. Store all other components at room temperature.
 Other Notes    Small volumes of 96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for 96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit

   Background: Normal primary cells proliferate in culture for a limited number of population doublings prior to undergoing terminal growth arrest and acquiring a senescent phenotype. This finite life span correlates with the age of the organism and with the life expectancy of the species from which the cells were obtained; such that the older the age or the shorter the life span, the less the ability of the cells to undergo population doubling. Senescent cells are characterized by an irreversibleG1 growth arrest involving the repression of genes that drive cell cycle progression and the upregulation of cell cycle inhibitors like p16INK4a, p53, and its transcriptional target, p21CIP1. They are resistant to mitogen-induced proliferation, and assume a characteristic enlarged, flattened morphology. Research into the pathways that positively regulate senescence and ways cells bypass senescence is therefore critical in understanding carcinogenesis. Normal cells have several mechanisms in place to protect against uncontrolled proliferation and tumorigenesis. Senescent cells show common biochemical markers such as expression of an acidic senescence-associated beta-galactosidase (SA-beta-Gal) activity. While senescence has been characterized primarily in cultured cells, there is also evidence that it occurs in vivo. Cells expressing markers of senescence such as SA-beta-Gal have been identified in normal tissues. The 96-well Cellular Senescence Assay Kit provides an easy-to-use and efficient method to determine the cellular senescence by measuring SA-beta-Gal activity using a fluorometric substrate. This quantitative assay uses cell lysate for both SA-beta-galactosidase activity determination and normalization of samples containing different cell numbers. Each kit provides sufficient quantities to perform up to 120 assays in a 96-well plate.
 Product Categories/Family for 96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit    Cell Based Assays; Cell Health Assays; Cellular Senescence
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 Typical Testing Data/Standard Curve (for reference only) of 96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit    96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS168468
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Product References and Citations for 96-well Cellular Senescence Assay (SA beta-Gal Activity) assay kit

   1. Current Protocols in Molecular Biology, John Wiley & Sons Press.
2. Campisi, J. (2000) In Vivo 14, 183-188.
3. Dimri, G. P., X. Lee, G. Basile, M. Acosta, G. Scott, C. Roskelley, E. E. Medrano, M. Linskens, I. Rubelj, O. Pereira-Smith, M. Peacocke, and J. Campisi. (1995) Proc. Natl. Acad. Sci. USA 92:9363-9367.
1. Lu, J. et al. (2014). SIRT1 counteracted the activation of STAT3 and NF-kappaB to repress the gastric cancer growth. Int J Clin Exp Med. 7:5050-5058.
2. Malhotra, D. et al. (2010). Global mapping of binding sites for Nrf2 identifies novel targets in cell survival response through ChIP-Seq profiling and network analysis. Nucleic Acids Res. 10.1093/nar/gkq212.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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