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CytoSelect MTT Cell Proliferation Assay Assay Kit

Scan QR to view Datasheet Catalog #    MBS168154
Unit / Price
960 Assays  /  $395 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CytoSelect MTT Cell Proliferation Assay, Assay Kit

 Also Known As   

CytoSelect MTT Cell Proliferation Assay

 Product Synonym Names    CytoSelect MTT Cell Proliferation Assay
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
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 Preparation and Storage    The MTT Cell Proliferation Assay Reagent is a clear yellow ready-to-use solution, and it should be stored at -20 degree C protected from light. Store the Detergent Solution at room temperature. If precipitate or turbidity is observed in the Detergent Solution, warm the solution to 37 degree C for 10-20 minutes and agitate to dissolve the precipitate prior to use.
 Product Note    Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CytoSelect MTT Cell Proliferation Assay assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for CytoSelect MTT Cell Proliferation Assay assay kit

  
Principle of the assay: CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation. The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1). An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Background: The measurement and monitoring of cell proliferation is an essential technique in any laboratory focused on cell-based research. This skill allows for the optimization of cell culture conditions as well as the determination of cytokine, growth factor, or hormone activity. More importantly, the cytostatic nature of anticancer compounds in toxicology testing, the efficacy of therapeutic chemicals in drug screening, and cell-mediated cytotoxicity can all be assessed through the quantification and monitoring of cell proliferation. Cell proliferation characteristics include cellular metabolic activity and cell membrane integrity. One method for measuring metabolic activity is to incubate the cells with a tetrazolium salt such as WST-1, which is cleaved into a colored formazan product by metabolically active cells. Similarly, the green fluorescent dye Calcein AM can measure intracellular esterase activity in proliferating live cells, which is another indicator of cell viability.
 Product Categories/Family for CytoSelect MTT Cell Proliferation Assay assay kit    Cell Based Assays; Cell Health Assays; Cell Proliferation Assays; MTT Cell Proliferation Assay
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 Testing Data of CytoSelect MTT Cell Proliferation Assay assay kit    CytoSelect MTT Cell Proliferation Assay assay kit Testing Data image
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 Testing Data #2 of CytoSelect MTT Cell Proliferation Assay assay kit    CytoSelect MTT Cell Proliferation Assay assay kit Testing Data #2 image
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Sample Manual Insert of MBS168154. Click to request current manual
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Product References and Citations for CytoSelect MTT Cell Proliferation Assay assay kit

   1. Jacobsen MD, Weil M, Raff MC. (1996) J Cell Biol 133, 1041.
2. Papadopoulos NG, Dedoussis GV, Spanakos G, Gritzapis AD, Baxevanis CN, Papamichail M. (1994) J Immunol Methods 177, 101.
3. Yamaori S, Ishii H, Chiba K, Yamamoto I, Watanabe K (2013) Toxicology 314, 251
4. Wang Y, Qu L, Gong L, Sun L, Gong R, Si J (2013) Cancer Biother Radiopharm. 28, 623
Zhang, L. et al. (2014). miR-125b Can Enhance Skin Tumor Initiation and Promote Malignant Progression by Repressing Differentiation and Prolonging Cell Survival. Genes Dev. 28:2532-2546.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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