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Free Fatty Acid Assay Kit

Scan QR to view Datasheet Catalog #    MBS168010
Unit / Price
100 Assays  /  $510 +1 FREE 8GB USB
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 Product Name   

Free Fatty Acid, Assay Kit

★Popular Item★
 Also Known As   

Free Fatty Acid Assay Kit (Colorimetric)

 Product Synonym Names    Free Fatty Acid Assay Kit (Colorimetric)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
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 Preparation and Storage    Store the entire kit at -80 degree C. Avoid multiple freeze/thaws by aliquoting. The NEM Reagent and Colorimetric Probe are light sensitive and should be maintained in amber tubes.
 Product Note    Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Free Fatty Acid assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Free Fatty Acid assay kit

   Background: Triglycerides (TAG) are a type of lipid in the blood, serving as an energy source and playing a key role in metabolism. Triglycerides are the digestive end product of breaking down dietary fats. Any extra carbohydrates and fats that are not immediately used are chemically converted into triglycerides. In the intestines, secreted enzyme lipases hydrolyze the triglyceride ester bond, yielding glycerol and free fatty acids (FFA) in a process called lipolysis. Additionally, hormones induce and regulate lipase activity in adipose tissue, resulting in changes to blood FFA levels. Free fatty acids then bind plasma albumin for circulation in the body, serving as a readily absorbed energy source for muscle, brain and other organ tissues. Measurement of free fatty acids has become useful in monitoring and diagnosis of several diseases and metabolic disorders (e.g. obesity, insulin resistance, diabetes, cancer). Free Fatty Acid Assay Kit measures non-esterified fatty acids (NEFA) in serum and plasma by a coupled enzymatic reaction system (ACS-ACOD Method). First, Acyl CoA Synthetase (ACS) catalyzes fatty acid acylation of coenzyme A. Next, the acyl-CoA product is oxidized by Acyl CoA Oxidase (ACOD), producing hydrogen peroxide which reacts with the kit's Colorimetric Probe (absorbance maxima of 570 nm). The Free Fatty Acid Assay Kit is a simple, colorimetric assay that quantitatively measures the free fatty acid concentration (non-esterified) in various samples using a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. The kit contains a palmitic acid standard and has a detection sensitivity limit of ~15 muM.
 Product Categories/Family for Free Fatty Acid assay kit    Metabolism Research; Lipoprotein Metabolism; Fatty Acid Metabolism
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 Testing Data of Free Fatty Acid assay kit    Free Fatty Acid assay kit Testing Data image
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 Testing Data #2 of Free Fatty Acid assay kit    Free Fatty Acid assay kit Testing Data #2 image
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Sample Manual Insert of MBS168010. Click to request current manual
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 Protein Family    Free fatty acid receptor

Product References and Citations for Free Fatty Acid assay kit

   1. Boden, G., Cheung, P., Stein, T.P. et al. (2002) Am J. Physiol. 283, E12-E-19.
2. Steinberg, H.O., Tarshoby, M., Monestel, R. et al. (1997) J. Clin. Invest. 100, 1230-1239.
3. Boden, G., Lebed, B. Schatz, M. et al. (2001) Diabetes 50, 1612-1617.
4. Boden, G., She, P., Mozzoli, M. et al. (2005) Diabetes 54, 917-927.
5. Kelley, D.E., Mokan, M., Simoneau, J.A., and Mandarino, L.J. (1993) J. Clin. Invest. 92, 91-98.
6. Lewis, G.F., Uffelman, K.D., Szeto, L.W., Weller, B., and Steiner, G. (1995) J. Clin. Invest. 95, 158-166.
Diané, A. et al. (2014). PACAP is essential for the adaptive thermogenic response of brown adipose tissue to cold exposure. J Endocrinol. 222:327-339.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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