View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List GST Colorimetric Activity Assay Kit Catalog # MBS841810 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name GST Colorimetric Activity Assay Kit Popular Item Full Product Name GST Colorimetric Activity Assay Kit Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Samples Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids Features & Benefits Simple procedure; takes only ~1-1.5 hoursFast and convenient Preparation and Storage At -20 degree CShelf Life: 12 months Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of GST Colorimetric Activity Assay Kit assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for GST Colorimetric Activity Assay Kit assay kit Product Information: Glutathione S-Transferase (GST) Colorimetric Activity Assay Kit: Detect GST Activity in Crude Cell Lysate or Purified Protein Fraction and Detect or Quantitate GST-tagged fusion recombinant protein. Simple, Absorbance-based in vitro Assay. Detection Limit < 1 mU.Background: Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. The GST Colorimetric Activity Assay Kit is based upon the GST-catalyzed reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene, which has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST. The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm. One unit of GST activity is defined as the amount of enzyme producing 1 umol of GS-DNB conjugate/min under the conditions of the assay. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein. Detect limit: Active GST < 4 mU.Summary: Detection method- Absorbance (380 nm) Species reactivity- Mammalian Applications- The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein. Product Categories/Family for GST Colorimetric Activity Assay Kit assay kit Cell Damage & Oxidative Stress; Glutathione & Related ProductsCell Damage & Oxidative Stress; Cell Damage & Oxidative Stress Assay KitsMetabolism Assays; Oxidative StressMetabolism Assays; Metabolism Assays (A-Z) Typical Testing Data/Standard Curve (for reference only) of GST Colorimetric Activity Assay Kit assay kit TOP Sample Manual Insert of MBS841810. 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