View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Glutathione (GSH/GSSG/Total) Assay Kit Catalog # MBS841503 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Glutathione (GSH/GSSG/Total), Assay Kit Popular Item Full Product Name Glutathione (GSH / GSSG / Total) Fluorometric Assay Kit Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Preparation and Storage At -20 degree CShelf Life: 12 months Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Glutathione (GSH/GSSG/Total) assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Glutathione (GSH/GSSG/Total) assay kit Background/Introduction: Glutathione is the major intracellular low-molecular-weight thiol that plays a critical role in cellular defense against oxidative stress in tissues and cells. Commercially available glutathione detection kits, such as the DTNB-enzyme cycling glutathione assay kit or the Monochlorobimane based assay kit hardly distinguish between reduced glutathione (GSH; FW: 307) and oxidized glutathione (GSSG; FW: 612). The Glutathione Detection Kit provides a unique, convenient tool for detecting GSH, GSSG, and total glutathione separately. In the assay, OPA, reacts with GSH (not GSSG), generating fluorescence, so GSH can be specifically quantified. Adding a reducing agent converts GSSG to GSH, so (GSH + GSSG) can be determined. To measure GSSG specifically, a GSH Quencher is added to remove GSH, preventing reaction with OPA (while GSSG is unaffected). Reducing agent is then added to destroy excess quencher and convert GSSG to GSH. Thus, GSSG can be specifically quantified. The kit provides a unique procedure and buffer formula to eliminate protein thiol interference and to stabilize GSH and GSSG in solution. The assay is easy to perform and detects 2-400 ng/ul of GSH, GSSG or total glutathione. Product Categories/Family for Glutathione (GSH/GSSG/Total) assay kit Cell Damage & Oxidative Stress; Glutathione & Related ProductsCell Damage & Oxidative Stress; Cell Damage & Oxidative Stress Assay KitsMetabolism Assays; Oxidative StressMetabolism Assays; Intermediary MetabolismMetabolism Assays; CoEnzymes & CofactorsMet Typical Testing Data/Standard Curve (for reference only) of Glutathione (GSH/GSSG/Total) assay kit TOP Sample Manual Insert of MBS841503. Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP