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GSH assay kit :: Glutathione Assay Kit

Scan QR to view Datasheet Catalog #    MBS480438
Unit / Price
1 Kit  /  $705 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glutathione (GSH), Assay Kit

 Also Known As   

Glutathione (GSH) Assay Kit

 Product Synonym Names    Colorimetric Assay for Glutathione
 Product Gene Name   

GSH assay kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
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 Preparation and Storage    4 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of GSH assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for GSH assay kit

   Introduction: The Analyte
Glutathione (gamma-glutamylcysteinylglycine or GSH) is a naturally occuring tripeptide whose
nucleophilic and
reducing properties play a central role in metabolic pathways, as well as in the antioxidant system of most
aerobic
cells.1 GSH plays a critical role as a coenzyme with a variety of enzymes including, glutathione peroxidase,
glutathione S-transferase, and thiol transferase. GSH also plays major roles in drug metabolism, calcium
metabolism, the ?-glutamyl cycle, blood platelet, and membrane functions. In addition, GSH is crucial to a
variety of
life processes, including the detoxification of xenobiotics, maintenance of the -SH level of proteins, thiol-
disulfide
exchange, removal of hydroperoxides and free radicals, and amino acid transport across membranes.
Physiological
values of intracellular GSH generally range from 1 to 10 mM. Although many methods have been
described for the
assay of GSH, the reliable ones are labor intensive and not easy to use.2).
Principles of the Procedures
This method is based on a chemical reaction which proceeds in two steps. The first step leads to the
formation of
substitution products (thioethers) between a patented reagent, R1 (4-chloro-1-methyl-7-trifluromethyl-
quinolinium
methylsulfate), and all mercaptans (RSH) which are present in the sample:
The second step is a ?-elimination reaction which takes place under alkaline conditions. This reaction is
mediated by
reagent R2 (30% NaOH) which specifically transforms the substitution product (thioether) obtained with
GSH into a
chromophoric thione which has a maximal absorbance wavelength at 400 nm:
This method makes it possible to specifically assay glutathione with only one sampling and one
colorimetric
measurement. A modification of this method can be used to assay other mercaptans. This is based on the
measurement of substitution products, thioethers, which absorb light at 356 nm in the absence of reagent
R
2.
Because of its simplicity, this method is especially well adapted to the assay of glutathione in large series
of
biological samples. The main advantage of the method is the specificity for glutathione and it does not
require an
enzyme as a reagent.
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 Testing Data of GSH assay kit    GSH assay kit Testing Data image
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 Testing Data #2 of GSH assay kit    GSH assay kit Testing Data #2 image
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 Testing Data #3 of GSH assay kit    GSH assay kit Testing Data #3 image
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 Testing Data #4 of GSH assay kit    GSH assay kit Testing Data #4 image
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 Testing Data #5 of GSH assay kit    GSH assay kit Testing Data #5 image
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 Testing Data #6 of GSH assay kit    GSH assay kit Testing Data #6 image
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 Testing Data #7 of GSH assay kit    GSH assay kit Testing Data #7 image
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Sample Manual Insert of MBS480438
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 Protein Family    Glutathione exchanger
 

Product References and Citations for GSH assay kit

   1. Dolphin D., Poulson R. and Avramovic O., Eds. (1989), Glutathione: Chemical, Biochemical and Medical
Aspects, Vols A & B, J. Wiley and Sons.
2. Anderson M.E. (1989), Enzymatic and chemical methods for the determination of Glutathione; In:
Glutathione:
chemical, biochemical and medical aspects, Vol. A, Dolphin D., Poulson R. and Avramovic O. Eds., John
Wiley and Sons, pp.339-365.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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