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Glutathione Assay Kit

Scan QR to view Datasheet Catalog #    MBS841532
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
100 Assays  /  $465 +1 FREE 8GB USB
 
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 Product Name   

Glutathione, Assay Kit

 Also Known As   

Glutathione Fluorometric Assay Kit

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
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 Samples    Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
 Features & Benefits    Simple two-step procedure; takes only 2 hours
Fast and convenient
The assay utilizes a dye that has a high affinity for glutathione. The unbound dye is almost nonfluorescent; whereas the dye fluoresces blue when bound to glutathione.
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 Preparation and Storage    At -70 degree C
Shelf Life: 12 months
 Product Note    Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Glutathione assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Glutathione assay kit

   Product Information: ApoGSHTM Glutathione Detection Kit: Rapid, Simple & Sensitive Assay to Detect Total Glutathione (oxidized & reduced). Detection Method: Fluorescence. 100 Assays.

Background: Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. MyBioSource's ApoGSH Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex./Em. = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.

Summary: Detection method- Fluorescence (Ex/Em 380/461 nm)
Species reactivity- Mammalian
Applications- The Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes.
 Product Categories/Family for Glutathione assay kit    Cell Damage & Oxidative Stress; Glutathione & Related ProductsMetabolism Assays; Oxidative StressMetabolism Assays; Intermediary MetabolismMetabolism Assays; Coenzymes & CofactorsMetabolism Assays; Metabolism Assays (A-Z)
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 Typical Testing Data/Standard Curve (for reference only) of Glutathione assay kit    Glutathione assay kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS841532. Click to request current manual
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 Protein Family    Glutathione exchanger
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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