Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Assay Kit rightarrow HDAC3  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • ESRRG - ERR Gamma Antibody
 • Fmoc-Leu-Rink-Amide MBHA Resin Resin
 • xylulokinase homolog (H. influenzae) (XYLB) ELISA Kit
 • a-Ketoglutaric acid Biochemical
 • Dectin-1 (Clec7a) Recombinant Protein
 • Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit
 • Biotin labeled rabbit IgG Antibody
 • Amylase Assay Kit
 • Rotavirus NCDV (all antigens) Antibody
 • Parathyroid Hormone (PTH) Antibody
 • HRI Antibody
 • CDKN2A Antibody
 • KYN (Kynurenine) ELISA Kit
 • Coxiella burnetii Phase II IgG Positive Control
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

HDAC3 assay kit

Scan QR to view Datasheet Catalog #    MBS841771
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
100 Assays  /  $605 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HDAC3, Assay Kit

 Also Known As   

HDAC3 Inhibitor Screening Kit (Fluorometric)

 Product Gene Name   

HDAC3 assay kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605166
Section Bar
Section Bar
Section Bar
 Samples    Testing candidate HDAC3 inhibitors
 Features & Benefits    Simple procedure; takes ~ 60 min
Fast and convenient
Kit contains the necessary reagents for testing putative HDAC3 inhibitors
Section Bar
 Preparation and Storage    At -20 degree C
Shelf Life: 12 months
 Product Note    Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HDAC3 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for HDAC3 assay kit

   Product Information: HDAC3 Inhibitor Screening Kit: High-Throughput Adaptable Fluorometric Assay to Screen HDAC3 Inhibitors. Rapid, Reliable, Simple & Sensitive. Includes Inhibitor Control, Trichostatin A. 100 Assays.

Background: Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In MyBioSource's HDAC-3 Inhibitor screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex=380/500 nm. In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.

Summary: Detection method- Fluorescence (Em/Ex = 380/500 nm)
Applications- Suitable for high throughput screening of HDAC3 inhibitors
 Product Categories/Family for HDAC3 assay kit    Epigenetics; HDACs; Assay KitsMetabolism Assays; Other Metabolism-Related AssaysMetabolism Assays; Metabolism Assays (A-Z)
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of HDAC3 assay kit    HDAC3 assay kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS841771. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for HDAC3. It may not necessarily be applicable to this product.
 NCBI GI #    13128862
 NCBI GeneID    8841
 NCBI Accession #    NP_003874.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003883.3 [Other Products]
Section Bar
 UniProt Secondary Accession #    O43268; Q9UEI5; Q9UEV0; D3DQE1 [Other Products]
 UniProt Related Accession #    O15379 [Other Products]
 Molecular Weight    49,111 Da
Table BarTOPTable Bar
 NCBI Official Full Name    histone deacetylase 3
 NCBI Official Synonym Full Names    histone deacetylase 3
 NCBI Official Symbol    HDAC3 [Similar Products]
 NCBI Official Synonym Symbols   
HD3; RPD3; RPD3-2
[Similar Products]
 NCBI Protein Information    histone deacetylase 3
 UniProt Protein Name    Histone deacetylase 3
 UniProt Synonym Protein Names   
RPD3-2; SMAP45
 Protein Family    Histone deacetylase
 UniProt Gene Name    HDAC3 [Similar Products]
 UniProt Synonym Gene Names    HD3 [Similar Products]
 UniProt Entry Name    HDAC3_HUMAN
Section Bar
 NCBI Summary for HDAC3    Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for HDAC3    HDAC3: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1, PRDM6 and SRY. Interacts with BTBD14B. Interacts with GLIS2. Interacts (via the DNA-binding domain) with NR2C1; the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Component of the Notch corepressor complex. Interacts with CBFA2T3 and NKAP. Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Interacts with and deacetylates MAPK14. Interacts with ZMYND15. Widely expressed. Belongs to the histone deacetylase family. HD type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Cell cycle regulation; Deacetylase; Apoptosis; EC 3.5.1.98; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; Golgi apparatus; transcriptional repressor complex; spindle microtubule; cytoplasm; histone deacetylase complex; plasma membrane; nucleus

Molecular Function: chromatin DNA binding; histone deacetylase binding; protein deacetylase activity; transcription factor binding; NAD-dependent histone deacetylase activity (H3-K9 specific); cyclin binding; protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); chromatin binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity

Biological Process: circadian rhythm; negative regulation of JNK cascade; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; regulation of mitotic cell cycle; regulation of protein stability; regulation of multicellular organism growth; cellular lipid metabolic process; chromatin modification; spindle assembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell cycle; protein amino acid deacetylation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; positive regulation of TOR signaling pathway; negative regulation of apoptosis
Table BarTOPTable Bar
 Research Articles on HDAC3    1. PML-mediated suppression of IL-6-induced STAT3 activation by disrupting interactions between STAT3 and HDAC3.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with HDAC3 assay kitDiseases associated with HDAC3 assay kit
 Reference Product  PubMed Publications
 HDAC4 assay kit  >69 publications with HDAC3 and HDAC4
 NCOR1 assay kit  >46 publications with HDAC3 and NCOR1
 HDAC5 assay kit  >44 publications with HDAC3 and HDAC5
 NCOR2 assay kit  >32 publications with HDAC3 and NCOR2
 RELA assay kit  >21 publications with HDAC3 and RELA
 EP300 assay kit  >12 publications with HDAC3 and EP300
 YY1 assay kit  >11 publications with HDAC3 and YY1
 TBL1X assay kit  >5 publications with HDAC3 and TBL1X
 TBL1XR1 assay kit  >4 publications with HDAC3 and TBL1XR1
 RBBP4 assay kit  >2 publications with HDAC3 and RBBP4
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >47 publications with HDAC3 and Inflammation
 Nervous System Diseases Antibodies  >34 publications with HDAC3 and Nervous System Diseases
 Breast Neoplasms Antibodies  >27 publications with HDAC3 and Breast Neoplasms
 Necrosis Antibodies  >25 publications with HDAC3 and Necrosis
 Adenocarcinoma Antibodies  >23 publications with HDAC3 and Adenocarcinoma
 Liver Neoplasms Antibodies  >14 publications with HDAC3 and Liver Neoplasms
 Lung Neoplasms Antibodies  >13 publications with HDAC3 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >12 publications with HDAC3 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >11 publications with HDAC3 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >9 publications with HDAC3 and Kidney Diseases
Organs/Tissues associated with HDAC3 assay kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >64 publications with HDAC3 and Blood
 Brain Antibodies  >60 publications with HDAC3 and Brain
 Liver Antibodies  >54 publications with HDAC3 and Liver
 Thyroid Antibodies  >47 publications with HDAC3 and Thyroid
 Bone Antibodies  >35 publications with HDAC3 and Bone
 Muscle Antibodies  >35 publications with HDAC3 and Muscle
 Lung Antibodies  >30 publications with HDAC3 and Lung
 Heart Antibodies  >24 publications with HDAC3 and Heart
 Nerve Antibodies  >21 publications with HDAC3 and Nerve
 Kidney Antibodies  >18 publications with HDAC3 and Kidney
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions