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HDAC6 assay kit :: Human HDAC6 Assay Kit

Scan QR to view Datasheet Catalog #    MBS846781
Typical Standard Curve/Testing Data
Unit / Price
100 Assays  /  $715 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HDAC6, Assay Kit

 Also Known As   

HDAC6 Inhibitor Screening Kit (Fluorometric)

 Product Gene Name   

HDAC6 assay kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    AF132609 mRNA
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 Species Reactivity    Human
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 Preparation and Storage    Store at -80 degree C for 12 months.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HDAC6 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for HDAC6 assay kit

   Background/Introduction: Histone Deacetylase 6 (HDAC6, EC 3.5.1.98), is a class IIb HDAC enzyme that deacetylates an epsilon-N-acetyl lysine of histone and non-histone protein substrates. It is a unique member of HDAC family as it contains two deacetylase domains that are proposed to function independently of each other. HDAC6 can shuttle between the nucleus and cytoplasm, suggesting potential extra-nuclear functions by regulating the acetylation status of non-histone substrates. HDAC6 also affects transcription and translation by regulating the heat-shock protein 90 (Hsp90) and stress granules. Elevated HDAC6 activity is associated with cell motility and catalyzes increases alpha-tubulin deacetylation, thus influencing cancer cell metastasis. In addition, mutations in HDAC6 gene have been associated with Alzheimer's disease. HDAC6-selective inhibitors are considered as promising targets against autoimmune, inflammatory and oncology diseases such as breast and ovarian cancer. HDAC6 Inhibitor Screening Kit utilizes deacetylase activity of HDAC6 towards a synthetic acetylated-peptide substrate resulting in the release of an AFC fluorophore, which can be easily quantified using a conventional microplate reader. In the presence of an HDAC6-selective inhibitor (Tubacin), the deacetylase activity of HDAC6 is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HDAC6.
 Product Categories/Family for HDAC6 assay kit    Epigenetics
 Application Notes for HDAC6 assay kit    Rapid; high-throughput screening of drugs and novel ligands. ; Development of structure-activity relationship (SAR) models to predict HDAC6 interaction liability of novel compounds.
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 Typical Standard Curve/Testing Data of HDAC6 assay kit    HDAC6 assay kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS846781. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HDAC6. It may not necessarily be applicable to this product.
 NCBI GI #    46623327
 NCBI GeneID    10013
 NCBI Accession #    AAH69243.1 [Other Products]
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 UniProt Secondary Accession #    O94975; Q6NT75; Q7L3E5; Q96CY0 [Other Products]
 UniProt Related Accession #    Q9UBN7 [Other Products]
 Molecular Weight    114,361 Da
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 NCBI Official Full Name    HDAC6 protein
 NCBI Official Synonym Full Names    histone deacetylase 6
 NCBI Official Symbol    HDAC6 [Similar Products]
 NCBI Official Synonym Symbols   
HD6; JM21; CPBHM; PPP1R90
[Similar Products]
 NCBI Protein Information    histone deacetylase 6
 UniProt Protein Name    Histone deacetylase 6
 UniProt Gene Name    HDAC6 [Similar Products]
 UniProt Synonym Gene Names    KIAA0901; HD6 [Similar Products]
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 NCBI Summary for HDAC6    Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HDAC6    HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily.

Protein type: Deacetylase; EC 3.5.1.98; Nuclear receptor co-regulator; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: axon; caveola; cytoplasm; cytosol; dendrite; dynein complex; histone deacetylase complex; inclusion body; leading edge; microtubule; microtubule associated complex; multivesicular body; nucleoplasm; nucleus; perikaryon; perinuclear region of cytoplasm

Molecular Function: alpha-tubulin binding; beta-catenin binding; enzyme binding; histone deacetylase activity; histone deacetylase binding; Hsp90 protein binding; microtubule binding; misfolded protein binding; polyubiquitin binding; protein binding; tau protein binding; tubulin deacetylase activity; ubiquitin protein ligase binding

Biological Process: histone deacetylation; intracellular protein transport; lysosome localization; misfolded or incompletely synthesized protein catabolic process; negative regulation of oxidoreductase activity; negative regulation of protein complex disassembly; negative regulation of proteolysis; negative regulation of transcription, DNA-dependent; positive regulation of signal transduction; protein amino acid deacetylation; regulation of autophagy; regulation of gene expression, epigenetic; regulation of macroautophagy; regulation of protein stability; regulation of receptor activity; response to misfolded protein; response to organic substance; response to toxin

Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia
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 Research Articles on HDAC6    1. The authors characterized the histone deacetylase 6-interacting proteins in LNCaP metastatic prostate cancer cells and found that histone deacetylase 6 interacts with proteins involved in several cellular processes, including autophagy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HDAC6 assay kitPathways associated with HDAC6 assay kit
 Reference Product  PubMed Publications
 SIRT2 assay kit  >8 publications with HDAC6 and SIRT2
 HDAC11 assay kit  >7 publications with HDAC6 and HDAC11
 VCP assay kit  >5 publications with HDAC6 and VCP
 EP300 assay kit  >2 publications with HDAC6 and EP300
 RUNX2 assay kit  >1 publications with HDAC6 and RUNX2
 BRMS1 assay kit  >1 publications with HDAC6 and BRMS1
 BCOR assay kit  >1 publications with HDAC6 and BCOR
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Signaling Events Mediated By HDAC Class I Pathway antibodies  Signaling Events Mediated By HDAC Class I Pathway Diagram
 Signaling Events Mediated By HDAC Class II Pathway antibodies  Signaling Events Mediated By HDAC Class II Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram
Diseases associated with HDAC6 assay kitOrgans/Tissues associated with HDAC6 assay kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >104 publications with HDAC6 and Neoplasms
 Nervous System Diseases Antibodies  >31 publications with HDAC6 and Nervous System Diseases
 Breast Neoplasms Antibodies  >28 publications with HDAC6 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >16 publications with HDAC6 and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >15 publications with HDAC6 and Disease Models, Animal
 Lymphoma Antibodies  >13 publications with HDAC6 and Lymphoma
 Inflammation Antibodies  >11 publications with HDAC6 and Inflammation
 Multiple Myeloma Antibodies  >8 publications with HDAC6 and Multiple Myeloma
 Fibrosis Antibodies  >8 publications with HDAC6 and Fibrosis
 Adenocarcinoma Antibodies  >7 publications with HDAC6 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >22 publications with HDAC6 and Brain
 Blood Antibodies  >20 publications with HDAC6 and Blood
 Prostate Antibodies  >12 publications with HDAC6 and Prostate
 Lung Antibodies  >12 publications with HDAC6 and Lung
 Bone Antibodies  >9 publications with HDAC6 and Bone
 Kidney Antibodies  >9 publications with HDAC6 and Kidney
 Muscle Antibodies  >8 publications with HDAC6 and Muscle
 Thyroid Antibodies  >6 publications with HDAC6 and Thyroid
 Skin Antibodies  >5 publications with HDAC6 and Skin
 Heart Antibodies  >5 publications with HDAC6 and Heart
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