View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List MitoPT TMRM assay kit :: MitoPT TMRM Mitochondrial Depolarization Assay Kit Catalog # MBS258008 Unit / Price 500 Tests / $410 +1 FREE 8GB USB 5x500 Tests / $1,860 +2 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name MitoPT TMRM Mitochondrial Depolarization, Assay Kit Popular Item Full Product Name MitoPT TMRM Mitochondrial Depolarization Assay Kit Product Gene Name MitoPT TMRM assay kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Reagents TMRM Sample Protocol Culture cells up to 1 x 10^6 cells/mL. Expose cells to apoptosis inducing agent or metabolic stress environment using your experimental protocol. Reconstitute the TMRM reagent with 100 uL DMSO to form the stock concentrate (may be frozen for future use). Dilute the stock concentrate with 1X PBS, cell culture media, or wash buffer to achieve a 10 uM intermediate stock for spiking cell aliquots. Spike cell cultures with 10 uM TMRM to achieve a 20-200 nM TMRM concentration. Incubate cells in TMRM (20-200 nM) solution, 15-20 minutes at 37 degree C in dark. Wash cells once in wash buffer if using > 50 nM TMRM staining concentration. Analyze data using a fluorescence microscope, plate reader, or flow cytometer. Target Mitochondrial Depolarization Excitation / Emission 548 nm / 573 nm Method of Analysis Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader Types of Samples Cell Culture, Tissue Preparation and Storage Mixed Storage Conditions: CCCP at -20 degree COther Kit Components at 2 - 8 degree C Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of MitoPT TMRM assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for MitoPT TMRM assay kit Description: MitoPT TMRM assay kits utilize the well-characterized, lipophilic but slightly cationic fluorescent dye TMRM to detect mitochondrial membrane permeability. Use of the MitoPT TMRM kit allows for the easy distinction of apoptotic or mitochondrial membrane-compromised cells from non-apoptotic cells with polarized mitochondria. Cells with polarized mitochondria will fluoresce bright red (em max: 573 nm); cells with membrane- compromised cells will exhibit a dimished fluorescence or no signal. The MitoPT TMRM mitochondrial membrane permeability assay can be used in conjunction with existing apoptosis or metabolic stress protocols. Each kit includes the TMRM potentiometric dye reagent, a 10X wash buffer for removing any excess TMRM dye after staining, and the generic mitochondrial membrane depolarizer, carbonylcyanide m- chlorophenylhydrazone (CCCP). TMRM-stained cells can be analyzed using flow cytometry, fluorescence microscopy, and fluorescence plate reader evaluation methodology.Background: Detect Mitochondrial Permeability Transition and Mitochondrial Membrane Depolarization with the MitoPT TMRM Assay ICT's MitoPT TMRM Assay utilizes the well-characterized potentiometric fluorescent dye tetramethylrhodamine methyl ester (TMRM) to detect mitochondrial permeability and membrane depolarization in whole, living cells. The rhodamine-based TMRE and TMRM dyes offered in ICT's MitoPT cell-based assay kits are categorized as slow membrane redistribution dyes. The lipophilic structures of hese dyes allow them to easily penetrate cell and mitochondrial lipid bilayer membrane barriers. Their potentiometric fluorescent properties allow for the detection of the mitochondrial permeability transition in whole cells via fluorescence analysis methods. Product Categories/Family for MitoPT TMRM assay kit Apoptosis Assays/Apoptosis Detection Typical Testing Data/Standard Curve (for reference only) of MitoPT TMRM assay kit TOP Sample Manual Insert of MBS258008. Product References and Citations for MitoPT TMRM assay kit Plasek, J. and K. Sigler. 1996. Slow fluorescent indicators of membrane potential: a survey of different approaches to probe response analysis. J. Photoch. Photobio. B. 33: 101-124. Ehrenberg, B., V. Montana, M. Wei, J.P. Wuskell, and L.M. Loew. 1988. Membrane potential can be determined in individual cells from the Nernstian distribution of cationic dyes. Biophys. J. 53: 785-794. Waggoner, A.S. 1979. Dye indicators or membrane potential. Ann Rev. Biophys Bioeng. 8: 47-68. o, R.C. and L.W. Grotyohann. 1999. Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives. Biophys. J. 76: 469-477. Wlodkowic, D., J. Skommer, and J. Pelkonen. 2006. Multiparametric analysis of HA14-1 induced apoptosis in follicular lymphoma cells. Leukemia Res. 30: 1187-1192. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. 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