Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our
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Other Notes
Small volumes of OxiSelect Comet assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
OxiSelect Comet assay kit
Principle of the Assay: OxiSelect 96-Well Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. First, individual cells are mixed with molten agarose before application to the OxiSelect 96-Well Comet Slide. These embedded cells are then treated with a lysis buffer and alkaline solution, which relaxes and denatures the DNA. Finally, the samples are electrophoresed in a horizontal chamber to separate intact DNA from damaged fragments. Following electrophoresis, the samples are dried, stained with a DNA dye, and visualized by epifluorescence microscopy. Under these conditions, the damaged DNA (containing cleavage and strand breaks) will migrate further than intact DNA and produce a comet tail shape (see Figure 1). Each kit provides sufficient reagents to perform up to 96 assays.
Background: DNA damage, due to environmental factors and normal metabolic processes inside the cell, occurs at a rate of 1,000 to 1,000,000 molecular lesions per cell per day. While this counts for only a small part of the human genome's approximately 6 billion bases (3 billion base pairs), unrepaired lesions to critical genes can impede a cell's ability to carry out its function and appreciably increase the likelihood of cancer. The comet assay, or single cell gel electrophoresis assay (SCGE), is a common technique for measurement of DNA damage in individual cells. Under an electrophoretic field, damaged cellular DNA (containing fragments and strand breaks) is separated from intact DNA, yielding a classic comet tail shape under the microscope. Extent of DNA damage is usually visually estimated by comet tail measurement; however, image analysis software is also available for measuring various parameters. The OxiSelect 96-Well Comet Assay is a fast and sensitive kit for the measurement of cellular DNA damage. Each kit provides sufficient reagents to perform up to 96 assays
Product References and Citations for
OxiSelect Comet assay kit
1. Ostling, O., and Johanson, K. J. (1984). Micro gel electrophoretic study of radiation induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123, 291-298.
2. Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. (1988). A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell. Res. 175, 184-191.
3. Olive, P. L., Banath, J. P., and Durand, R. E. (1990a). Heterogeneity in radiation induced DNA damage and repair in tumor and normal cells using the "Comet" assay. Radiat. Res. 122, 86-94.
4. De Boeck, M., Touil, N., De Visscher, G., Vande, P. A., and Kirsch-Volders, M. (2000). Validation and implementation of an internal standard in Comet assay. Mutat. Res. 469, 181-197.
Tyagi, A. et al. (2011). Resveratrol Selectively Induces DNA Damage, Independent of Smad4 Expression, in its Efficacy Against Human Head and Neck Squamous Cell Carcinoma. Clin. Cancer Res. 17:5402-5411.
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