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Sphingomyelin Assay Kit

Scan QR to view Datasheet Catalog #    MBS168619
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Assays  /  $495 +1 FREE 8GB USB
 
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 Product Name   

Sphingomyelin, Assay Kit

★Popular Item★
 Also Known As   

Sphingomyelin Assay Kit

 Product Synonym Names    Sphingomyelin Assay Kit
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
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 Preparation and Storage    Upon receipt, store the Sphingomyelin Standard, Fluorescence Probe, HRP, Alkaline Phosphatase and Choline Oxidase at -20 degree C. The Fluorescence Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles. Store the remaining kit components at 4 degree C.
 Product Note    Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Sphingomyelin assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Sphingomyelin assay kit

   Principle of the Assay: Sphingomyelin Assay Kit measures the sphingomyelin present within serum, plasma, or tissue samples. The assay is based on the enzyme driven reaction that will detect sphingomyelin via the enzymatic activity of sphingomyelinase, alkaline phosphatase, and choline oxidase. First, sphingomyelinase hydrolyzes sphingomyelin into phosphorylcholine and the lipid ceramide. Alkaline phosphatase breaks down phosphorylcholine into choline, which is then oxidized by choline oxidase to produce hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorescence probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of sphingomyelin standard within the 96-well microtiter plate format. Samples and standards are incubated for 60 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).

Background/Introduction: Phospholipids are important structural lipids that are the major component of cell membranes and lipid bilayers. Phospholipids contain a hydrophilic head and a hydrophobic tail which give the molecules their unique characteristics. Most phospholipids contain one diglyceride, a phosphate group, and one choline group. Sphingomyelin (ceramide phosphorylcholine) is a sphingolipid found in eukaryotic cell membranes and lipoproteins. Sphingomyelin usually consists of a ceramide and phosphorylcholine molecule where the ceramide core comprises of a fatty acid bonded via an amide bond to a sphingosine molecule. There is a polar head group which is either phosphphoethanolamine or phosphocholine. Sphingomyelin represents about 85% of all sphingolipids and makes up about 10-20% of lipids within the plasma membrane. Sphingomyelin is involved in signal transduction and is highly concentrated in the myelin sheath around many nerve cell axons. The plasma membranes of many cells are rich with sphingomyelin. Sphigolipids are synthesized in a pathway that originates in the ER and is completed in the Golgi apparatus. Many of their functions are done in the plasma membranes and endosomes. Sphingomyelin is converted to ceramide via sphingomyelinases. Ceramides have been implicated in signaling pathways that lead to apoptosis, differentiation and proliferation. Sphingomyelins have been implicated in the pathogenesis of atherosclerosis, inflammation, necrosis, autophagy, senescence, stress response as well as other signaling disease states. Niemann-Pick disease is an inherited disease where deficiency of sphingomyelinase activity results in sphingomyelin accumulating in cells, tissues, and fluids. Other sphingolipid diseases are Fabry disease, Gaucher disease, Tay-Sachs disease, Krabbe disease and Metachromatic leukodystrophy. Sphingomyelin Assay Kit is a simple fluorometric assay that measures the amount of sphingomyelin present in plasma or serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, sphingomyelin standards and unknown samples. Sample sphingomyelin concentrations are determined by comparison with a known sphingomyelin standard.
 Product Categories/Family for Sphingomyelin assay kit    Metabolism Research; Lipoprotein Metabolism; Phospholipid Assays
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 Typical Testing Data/Standard Curve (for reference only) of Sphingomyelin assay kit    Sphingomyelin assay kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS168619. Click to request current manual
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 Protein Family    Sphingomyelinase
 

Product References and Citations for Sphingomyelin assay kit

   1. Hojjati, M.R., et al. (2006) J. Lipid Res. 47(3): 673-676.
2. Jiang, X.-C., et al. (2000) Arterioscler. Thromb. Vasc. Biol. 20(12): 2614-2618.
3. Riboni, L., et al. (1997) Prog. Lipid Res. 36: 153-195.
4. Testi, R. (1996) Trends Biochem. Sci. 21: 468-471.
Winkler, E.A. et al. (2014). Blood-spinal cord barrier disruption contributes to early motor-neuron degeneration in ALS-model mice. PNAS 111:E1035-E1042.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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