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PROK biochemical enzyme :: Proteinase K (PROK) Biochemical Enzyme

Scan QR to view Datasheet Catalog #    MBS7053447
Unit / Price
0.1 mg  /  $85 +1 FREE 8GB USB
0.2 mg  /  $110 +1 FREE 8GB USB
0.5 mg  /  $180 +1 FREE 8GB USB
1 mg  /  $275 +1 FREE 8GB USB
2 mg  /  $525 +1 FREE 8GB USB
5 mg  /  $1,265 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Proteinase K (PROK), Biochemical Enzyme

★Popular Item★
 Also Known As   

Proteinase K (PROK) Recombinant Protein

 Product Gene Name   

PROK biochemical enzyme

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence Positions    Full length expression
 Sequence    MRLSVLLSLL PLALGAPAVE QRSEAAPLIE ARGEMVANKY IVKFKEGSAL SALDAAMEKI SGKPDHVYKN VFSGFAATLD ENMVRVLRAH PDVEYIEQDA VVTINAAQTN APWGLARISS TSPGTSTYYY DESAGQGSCV YVIDTGIEAS HPEFEGRAQM VKTYYYSSRD GNGHGTHCAG TVGSRTYGVA KKTQLFGVKV LDDNGSGQYS TIIAGMDFVA SDKNNRNCPK GVVASLSLGG GYSSSVNSAA ARLQSSGVMV AVAAGNNNAD ARNYSPASEP SVCTVGASDR YDRRSSFSNY GSVLDIFGPG TSILSTWIGG STRSISGTSM ATPHVAGLAA YLMTLGKTTA ASACRYIADT ANKGDLSNIP FGTVNLLAYN NYQA
 3D Structure    ModBase 3D Structure for P06873
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 Host    Yeast
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 Purity/Purification    >=95% SDS-PAGE
 Form/Format    Lyophilized powder
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 Specific Activity    >=30U/mg
 Gene    Tritirachium album Liber
 Product Note    Applications are user defined. Product is developed and quality control tested in house, data is not provided. The researcher needs to establish and confirm the suitability of the product for their application.
 Tag type    His Tag
  Tag Position    C-terminal
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 Preparation and Storage    Store at -20 or -80 degree C.
Avoid repeated freeze/thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of PROK biochemical enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for PROK biochemical enzyme

   Proteinase K is an extracellular serine endoproteinase produced by Tritirachium album Limber. Proteinase K belongs to the peptidase family S8. Its predominant cleavage site is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked ?-amino groups. Proteinase K is a broad-spectrum protease that functions at wide pH and temperature ranges. The optimum pH and temperature for the activity of this protease were approximately pH 7.5-8.0 and 55C, respectively. In addition, 0.2-1% sodium dodecyl sulfate (SDS) and 10mM carbamide can activate its activity.

The combination of these unique features makes Proteinase K as an important tool in scientific research. Proteinase K can be used in the preparation of biological macromolecules by degrading the constitutive proteins of cells and removing nucleases such as DNases and RNases for RT-PCR. In nucleic acid vaccine and molecular diagnosis, it is also widely used for degrading proteinaceous impurities during the isolation of nucleic acids from biological samples.

The latest launch of high quality of Proteinase K shows its high purity and high enzyme activity by biochemical platform testing. Through repeated inspection, all the data show our company produced Proteinase K without RNA, DNA, RNAse and DNAse residues.
 Applications Tested/Suitable for PROK biochemical enzyme   

Raw Materials for Genomic DNA, Raw Materials for RNA Purification, RNA purification kits, IHC, ISH and other

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 Calibration Curve of PROK biochemical enzyme    According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn. Then, the enzyme activity was 30U/mg by calculating (Fig.1).
Unit definition: One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37C
PROK biochemical enzyme Calibration Curve image
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  Protein Purity of PROK biochemical enzyme    The purity and the molecular weight of MBS7053447 proteinase K were over 95% and 28.9 kDa by SDS-PAGE, respectively (Fig.2).
PROK biochemical enzyme  Protein Purity image
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  Detection of Nucleic Acid Residue of PROK biochemical enzyme    By agarose gel electrophoresis to detect nucleic acid, figure 3 shows MBS7053447produced proteinase K without RNA, DNA residues (Fig.3).
PROK biochemical enzyme  Detection of Nucleic Acid Residue image
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  Detection of DNAse Residue of PROK biochemical enzyme    DNAses remained in lyophilized powder were detected by agarose gel electrophoresis , figure 4 shows MBS7053447 produced proteinase K without DNAse residue after 6h of incubation at 37C (Fig.4).
PROK biochemical enzyme  Detection of DNAse Residue image
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  Detection of RNAse Residue of PROK biochemical enzyme    Agarose gel electrophoresis was performed to check whether there was residual RNase. Figure 5 shows MBS7053447 produced proteinase K without RNAse residue after 16h of incubated at 25C (Fig.5).
PROK biochemical enzyme  Detection of RNAse Residue image
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NCBI/Uniprot data below describe general gene information for PROK. It may not necessarily be applicable to this product.
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 UniProt Primary Accession #    P06873 [Other Products]
 Molecular Weight    65,531 Da
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 NCBI Official Full Name    proteinase K
 UniProt Protein Name    Proteinase K
 UniProt Synonym Protein Names   
Proteinase K
 Protein Family    Proteinase
 UniProt Gene Name    PROK [Similar Products]
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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