|
| Product Name
|
|
AKT (AKT1), Blocking Peptide
|
| Also Known As
|
|
AKT Blocking Peptide
|
| Product Synonym Names
|
|
PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Antibody/Peptide Pairs
|
|
AKT peptide (MBS822967) is used for blocking the activity of AKT antibody (MBS820839)
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
|
| OMIM
|
|
phenotype 615109
|
| 3D Structure
|
|
ModBase 3D Structure for P31749
|
|
| Host
|
|
Synthetic
|
| Species Reactivity
|
|
Human, Mouse, Rat, Bovine, Pig, Sheep
|
|
|
| Purity/Purification
|
|
>85%
|
| Form/Format
|
|
Lyophilized powder
|
|
|
| Quality Control
|
|
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
|
|
|
| Preparation and Storage
|
|
Shipped at 4 degree C. Store at -20 degree C for one year.
|
| Other Notes
|
|
Small volumes of AKT1 blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
|
TOP
|
|
|
|
|
The peptide is used to block Anti-AKT Antibody reactivity.
|
|
| Applications Tested/Suitable for AKT1 blocking peptide
|
|
Blocking (BL)
|
| Application Notes for AKT1 blocking peptide
|
|
Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for AKT1. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
62241013
|
| NCBI GeneID
|
|
207
|
| NCBI Accession #
|
|
NP_001014431.1
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_001014431.1
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
P31749
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
Q9BWB6; B2RAM5; B7Z5R1
|
[Other Products]
|
| UniProt Related Accession #
|
|
P31749
|
[Other Products]
|
| Molecular Weight
|
|
48,347 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
RAC-alpha serine/threonine-protein kinase
|
| NCBI Official Synonym Full Names
|
|
v-akt murine thymoma viral oncogene homolog 1
|
| NCBI Official Symbol
|
|
AKT1
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
|
[Similar Products]
|
| NCBI Protein Information
|
|
RAC-alpha serine/threonine-protein kinase; AKT1m; PKB alpha; RAC-PK-alpha; proto-oncogene c-Akt; protein kinase B alpha; rac protein kinase alpha
|
| UniProt Protein Name
|
|
RAC-alpha serine/threonine-protein kinase
|
| UniProt Synonym Protein Names
|
|
Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
|
| Protein Family
|
|
AKT-interacting protein
|
| UniProt Gene Name
|
|
AKT1
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
PKB; RAC; PKB; PKB alpha
|
[Similar Products]
|
| UniProt Entry Name
|
|
AKT1_HUMAN
|
|
|
| NCBI Summary for AKT1
|
|
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
|
|
|
| UniProt Comments for AKT1
|
|
Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.
Protein type: Protein kinase, AGC; Oncoprotein; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family
Chromosomal Location of Human Ortholog: 14q32.32
Cellular Component: microtubule cytoskeleton; nucleoplasm; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; cytosol; nucleus
Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity
Biological Process: negative regulation of JNK cascade; regulation of myelination; positive regulation of nitric oxide biosynthetic process; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; regulation of cell migration; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; response to heat; T cell costimulation; positive regulation of fat cell differentiation; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of transcription factor activity; regulation of nitric-oxide synthase activity; response to oxidative stress; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; positive regulation of cellular protein metabolic process; regulation of glycogen biosynthetic process; positive regulation of glycogen biosynthetic process; negative regulation of cell size; glucose transport; negative regulation of caspase activity; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; inflammatory response; nitric oxide biosynthetic process; cell differentiation; activated T cell apoptosis; aging; negative regulation of proteolysis; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; positive regulation of peptidyl-serine phosphorylation; endocrine pancreas development; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; cell proliferation; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of vasoconstriction; insulin receptor signaling pathway; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; hyaluronan metabolic process
Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer
|
|
TOP
|
|
| Research Articles on AKT1
|
|
1. Data suggest that the ribosomal S6 kinase : protein kinase B (AKT) phosphorylation ratio could be useful as a biomarker of target inhibition by RAD001.
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with AKT1 blocking peptide | Pathways associated with AKT1 blocking peptide |
|
|
|
| Diseases associated with AKT1 blocking peptide | Organs/Tissues associated with AKT1 blocking peptide |
|
|
|
|
|
|
TOP
|
Blocking Peptide 
