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BRAF blocking peptide :: B-raf Blocking Peptide

Scan QR to view Datasheet Catalog #    MBS542476 BRAF blocking peptide
Unit / Price
0.25 mg Peptide  /  $250 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

B-raf (BRAF), Blocking Peptide

 Also Known As   

B-raf Peptide

 Product Synonym Names    94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B RAF1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; cRmil; Murine sarcoma viral v raf oncogene homolog B1; NS7; Oncogen BRAF; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v-Raf murine sarcoma viral oncogene homolog B1 antibody
 Product Gene Name   

BRAF blocking peptide

[Similar Products]
 Antibody/Peptide Pairs    B-raf peptide (MBS542476) is used for blocking the activity of B-raf antibody (MBS541081)
 Antibody/Peptide Pairs    B-raf peptide (MBS542476) is used for blocking the activity of B-raf antibody (MBS541082)
 Antibody/Peptide Pairs    B-raf peptide (MBS542476) is used for blocking the activity of B-raf antibody (MBS541083)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    114500
 3D Structure    ModBase 3D Structure for P15056
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 Form/Format    Antigenic Blocking Peptide
 Concentration    Quantity: 250 ug; Volume: 100 ul (lot specific)
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 Immunogen    Synthetic peptide taken within amino acid region 700-750 on human serine/threonine-protein kinase B-raf protein.
 Expression    Brain and testis.
 Molecular Function    Kinase, Serine/threonine-protein kinase, Transferase
 Structure    Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2.
 Subcellular Location    Nucleus, Cytoplasm, Cell membrane
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 Preparation and Storage    -20 degree C for long term storage
 Other Notes    Small volumes of BRAF blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for BRAF blocking peptide

   Antigenic Blocking Peptide Serine/threonine-protein kinase B-raf Antibody
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
 Applications Tested/Suitable for BRAF blocking peptide   

Immunodepletion, Immunocompetition

 Application Notes for BRAF blocking peptide    ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
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NCBI/Uniprot data below describe general gene information for BRAF. It may not necessarily be applicable to this product.
 NCBI GI #    33188459
 NCBI GeneID    673
 NCBI Accession #    NP_004324.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004333.4 [Other Products]
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 UniProt Primary Accession #    P15056 [Other Products]
 UniProt Secondary Accession #    Q13878; Q3MIN6; Q9UDP8; Q9Y6T3; A4D1T4; B6HY61; B6HY62; B6HY63; B6HY64; B6HY65; B6HY66 [Other Products]
 UniProt Related Accession #    P15056 [Other Products]
 Molecular Weight    84,437 Da [Similar Products]
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 NCBI Official Full Name    serine/threonine-protein kinase B-raf
 NCBI Official Synonym Full Names    B-Raf proto-oncogene, serine/threonine kinase
 NCBI Official Symbol    BRAF [Similar Products]
 NCBI Official Synonym Symbols   
NS7; BRAF1; RAFB1; B-RAF1
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase B-raf
 UniProt Protein Name    Serine/threonine-protein kinase B-raf
 UniProt Synonym Protein Names   
Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    BRAF [Similar Products]
 UniProt Synonym Gene Names    BRAF1; RAFB1 [Similar Products]
 UniProt Entry Name    BRAF_HUMAN
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 NCBI Summary for BRAF    This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BRAF    BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.1; Oncoprotein; TKL group; RAF family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; mitochondrion; neuron projection; nucleus; plasma membrane

Molecular Function: ATP binding; calcium ion binding; identical protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein binding; protein heterodimerization activity; protein kinase activity; protein serine/threonine kinase activity; small GTPase binding

Biological Process: activation of MAPKK activity; CD4-positive, alpha beta T cell differentiation; glucose transport; MAPKKK cascade; myeloid progenitor cell differentiation; negative regulation of apoptosis; negative regulation of neuron apoptosis; organ morphogenesis; positive regulation of axon regeneration; positive regulation of axonogenesis; positive regulation of peptidyl-serine phosphorylation; positive regulation of stress fiber formation; positive T cell selection; protein amino acid phosphorylation; protein heterooligomerization; regulation of cell proliferation; response to cAMP; response to peptide hormone stimulus; somatic stem cell maintenance; thymus development; thyroid gland development; visual learning

Disease: Cardiofaciocutaneous Syndrome 1; Leopard Syndrome 3; Lung Cancer; Noonan Syndrome 1; Noonan Syndrome 7
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 Research Articles on BRAF    1. BRAF fusions are rare driver alterations in a wide variety of malignant neoplasms, but enriched in Spitzoid melanoma, pilocytic astrocytomas, pancreatic acinar and papillary thyroid cancers.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BRAF blocking peptideDiseases associated with BRAF blocking peptide
 Reference Product  PubMed Publications
 NRAS blocking peptide  >1159 publications with BRAF and NRAS
 HRAS blocking peptide  >244 publications with BRAF and HRAS
 MAP2K1 blocking peptide  >185 publications with BRAF and MAP2K1
 MAP2K2 blocking peptide  >86 publications with BRAF and MAP2K2
 RAF1 blocking peptide  >85 publications with BRAF and RAF1
 MAPK3 blocking peptide  >10 publications with BRAF and MAPK3
 KSR1 blocking peptide  >7 publications with BRAF and KSR1
 RAP1A blocking peptide  >2 publications with BRAF and RAP1A
 PRKCA blocking peptide  >1 publications with BRAF and PRKCA
 Disease Name  Pubmed Publications
 Melanoma Antibodies  >2944 publications with BRAF and Melanoma
 Neuroendocrine Tumors Antibodies  >2104 publications with BRAF and Neuroendocrine Tumors
 Colorectal Neoplasms Antibodies  >1549 publications with BRAF and Colorectal Neoplasms
 Thyroid Neoplasms Antibodies  >1174 publications with BRAF and Thyroid Neoplasms
 Microsatellite Instability Antibodies  >687 publications with BRAF and Microsatellite Instability
 Carcinogenesis Antibodies  >578 publications with BRAF and Carcinogenesis
 Lung Neoplasms Antibodies  >476 publications with BRAF and Lung Neoplasms
 Lung Diseases Antibodies  >438 publications with BRAF and Lung Diseases
 Nervous System Diseases Antibodies  >414 publications with BRAF and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >351 publications with BRAF and Neoplasms, Experimental
Organs/Tissues associated with BRAF blocking peptide
 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >1473 publications with BRAF and Thyroid
 Skin Antibodies  >1368 publications with BRAF and Skin
 Lung Antibodies  >694 publications with BRAF and Lung
 Blood Antibodies  >554 publications with BRAF and Blood
 Lymph Node Antibodies  >522 publications with BRAF and Lymph Node
 Brain Antibodies  >501 publications with BRAF and Brain
 Kidney Antibodies  >109 publications with BRAF and Kidney
 Prostate Antibodies  >68 publications with BRAF and Prostate
 Ovary Antibodies  >50 publications with BRAF and Ovary
 Pancreas Antibodies  >49 publications with BRAF and Pancreas
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