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Cell Extraction Buffer

Scan QR to view Datasheet Catalog #    MBS651654 Cell Extraction Buffer buffer
Unit / Price
100 mL  /  $420 +1 FREE 8GB USB
 
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 Product Name   

Cell Extraction Buffer

★Popular Item★
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Purity/Purification    Highly Purified
 Form/Format    Buffer Formulation:
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 Buffer Formulation    10mM Tris, pH 7.4
100mM NaCl
1mM EDTA
lmM EGTA
1mM NaF
20mM Na4P2O7
2mM Na3VO4
1% Triton X-1OO
10% glycerol
0.1% SDS
0.5% deoxycholate
 Important Note    This cell extraction buffer must be supplemented with 1 mM PMSF (not included) and protease inhibitor cocktail (not included) just prior to use.

For the PMSF addition, we recommend making a 0.3M stock in DMSO and adding sufficient volume for a final concentration of 1 mM (i.e. 17ul per 5ml cell extraction buffer). PMSF is very unstable and must be added just prior to use, even if added previously.
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 Preparation and Storage    Store at -70 degree C. Thaw this buffer on ice. This buffer is stable for 2-3 weeks at 4 degree C or for up to 6 months when apportioned into working aliquots and stored at -70 degree C.
 Other Notes    Small volumes of Cell Extraction Buffer buffer vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for Cell Extraction Buffer buffer    Molecular Biology; MB-Buffers
 Applications Tested/Suitable for Cell Extraction Buffer buffer   

ELISA (EL/EIA), Western Blot (WB)

 Application Notes for Cell Extraction Buffer buffer    Suitable for use in ELISA and Western blot.
Other applications have not been tested.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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