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AChE clia kit :: Rat AChE (Acetylcholinesterase) CLIA Kit

Scan QR to view Datasheet Catalog #    MBS2532286
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AChE (Acetylcholinesterase), CLIA Kit

 Also Known As   

Rat AChE (Acetylcholinesterase) CLIA Kit

 Product Synonym Names    ACEE; ARACHE; N-ACHE; YT; acetylhydrolase
 Product Gene Name   

AChE clia kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    100740
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 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinant AChE. No significant cross-reactivity or interference between AChE and analogues was observed.
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 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our CLIA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AChE clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for AChE clia kit

   Intended Uses: This CLIA kit applies to the in vitro quantitative determination of AChE concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to AChE. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AChE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of AChE. You can calculate the concentration of AChE in the samples by comparing the RLU value of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of AChE clia kit    AChE clia kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2532286. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AChE. It may not necessarily be applicable to this product.
 NCBI GI #    545225
 NCBI GeneID    43
 NCBI Accession #    AAC60618.1 [Other Products]
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 UniProt Secondary Accession #    Q16169; Q29S23; Q2M324; Q504V3; Q53F46; Q86TM9; Q86YX9; Q9BXP7; A4D2E2; B7ZKZ0; D6W5X7 [Other Products]
 UniProt Related Accession #    P22303 [Other Products]
 Molecular Weight    58,352 Da
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 NCBI Official Full Name    acetylcholinesterase, partial
 NCBI Official Synonym Full Names    acetylcholinesterase (Yt blood group)
 NCBI Official Symbol    ACHE [Similar Products]
 NCBI Official Synonym Symbols   
YT; ACEE; ARACHE; N-ACHE
[Similar Products]
 NCBI Protein Information    acetylcholinesterase
 UniProt Protein Name    Acetylcholinesterase
 UniProt Gene Name    ACHE [Similar Products]
 UniProt Synonym Gene Names    AChE [Similar Products]
 UniProt Entry Name    ACES_HUMAN
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 NCBI Summary for AChE    Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AChE    ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.7; Lipid Metabolism - glycerophospholipid; Membrane protein, GPI anchor; Hydrolase

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane; synapse; basal lamina; neuromuscular junction; cell junction; nucleus

Molecular Function: collagen binding; serine hydrolase activity; protein binding; protein homodimerization activity; cholinesterase activity; protein self-association; hydrolase activity; beta-amyloid binding; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: nervous system development; negative regulation of synaptic transmission, cholinergic; muscle development; acetylcholine catabolic process in synaptic cleft; acetylcholine catabolic process; glycerophospholipid biosynthetic process; osteoblast development; cell proliferation; synaptic transmission; synaptogenesis; positive regulation of protein secretion; retina development in camera-type eye; amyloid precursor protein metabolic process; neurotransmitter receptor biosynthetic process; receptor internalization; phospholipid metabolic process; phosphatidylcholine biosynthetic process; response to wounding; regulation of receptor recycling; DNA replication; cell adhesion; protein tetramerization; neurotransmitter biosynthetic process

Disease: Yt Blood Group Antigen
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 Research Articles on AChE    1. Data suggest that natural antisense RNA may play an important role in acetylcholinesterase (AChE) regulation via affecting the epigenetic modification in the AChE promoter region.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AChE clia kitPathways associated with AChE clia kit
 Reference Product  PubMed Publications
 JUN clia kit  >57671 publications with AChE and JUN
 CHAT clia kit  >905 publications with AChE and CHAT
 APP clia kit  >216 publications with AChE and APP
 COLQ clia kit  >89 publications with AChE and COLQ
 SLC44A1 clia kit  >1 publications with AChE and SLC44A1
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
Organs/Tissues associated with AChE clia kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >82664 publications with AChE and Blood
 Nerve Antibodies  >48941 publications with AChE and Nerve
 Muscle Antibodies  >44336 publications with AChE and Muscle
 Heart Antibodies  >42917 publications with AChE and Heart
 Brain Antibodies  >38556 publications with AChE and Brain
 Liver Antibodies  >14706 publications with AChE and Liver
 Kidney Antibodies  >13811 publications with AChE and Kidney
 Lung Antibodies  >12533 publications with AChE and Lung
 Intestine Antibodies  >12486 publications with AChE and Intestine
 Mouth Antibodies  >10628 publications with AChE and Mouth
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