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ADA clia kit :: Human ADA (Adenosine Deaminase) CLIA Kit

Scan QR to view Datasheet Catalog #    MBS2530446
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $535 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ADA (Adenosine Deaminase), CLIA Kit

 Also Known As   

Human ADA (Adenosine Deaminase) CLIA Kit

 Product Synonym Names    Adenosine Aminhydrolase
 Product Gene Name   

ADA clia kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    102700
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant ADA. No significant cross-reactivity or interference between ADA and analogues was observed.
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 Assay Type    Sandwich
 Detection Range    78.125-5000pg/mL
 Sensitivity    Min: 46.875pg/mL; Max: 5000pg/mL
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 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our CLIA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ADA clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ADA clia kit

   Intended Uses: This CLIA kit applies to the in vitro quantitative determination of ADA concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ADA. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ADA. You can calculate the concentration of ADA in the samples by comparing the RLU value of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ADA clia kit    ADA clia kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2530446. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ADA. It may not necessarily be applicable to this product.
 NCBI GI #    47078295
 NCBI GeneID    100
 NCBI Accession #    NP_000013.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000022.2 [Other Products]
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 UniProt Secondary Accession #    Q53F92; Q6LA59 [Other Products]
 UniProt Related Accession #    P00813 [Other Products]
 Molecular Weight    40,764 Da
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 NCBI Official Full Name    adenosine deaminase
 NCBI Official Synonym Full Names    adenosine deaminase
 NCBI Official Symbol    ADA [Similar Products]
 NCBI Protein Information    adenosine deaminase
 UniProt Protein Name    Adenosine deaminase
 UniProt Synonym Protein Names   
Adenosine aminohydrolase
 Protein Family    Regulatory protein
 UniProt Gene Name    ADA [Similar Products]
 UniProt Synonym Gene Names    ADA1 [Similar Products]
 UniProt Entry Name    ADA_HUMAN
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 NCBI Summary for ADA    This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ADA    ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm.

Protein type: Hydrolase; EC; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: dendrite cytoplasm; extracellular space; cell surface; cell soma; membrane; lysosome; cytoplasm; plasma membrane; cytosol; cell junction; external side of plasma membrane

Molecular Function: adenosine deaminase activity; protein binding; zinc ion binding; purine nucleoside binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; T cell activation; adenosine catabolic process; deoxyadenosine catabolic process; response to morphine; positive regulation of calcium-mediated signaling; purine salvage; histamine secretion; positive regulation of T cell differentiation in the thymus; purine ribonucleoside monophosphate biosynthetic process; response to vitamin E; regulation of cell-cell adhesion mediated by integrin; negative regulation of mature B cell apoptosis; positive regulation of T cell receptor signaling pathway; positive regulation of B cell proliferation; positive regulation of germinal center formation; hypoxanthine salvage; positive regulation of smooth muscle contraction; negative regulation of adenosine receptor signaling pathway; embryonic gut development; aging; placenta development; Peyer's patch development; response to drug; nucleobase, nucleoside and nucleotide metabolic process; dATP catabolic process; positive regulation of heart rate; liver development; negative regulation of leukocyte migration; purine base metabolic process; trophectodermal cell differentiation; purine nucleotide salvage; xanthine biosynthetic process; response to hydrogen peroxide; negative regulation of inflammatory response; response to hypoxia; inosine biosynthetic process; germinal center B cell differentiation; alveolus development; positive regulation of alpha-beta T cell differentiation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency
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 Research Articles on ADA    1. ADA rs73598374-A carriers showed lower telomerase activity and shorter leukocyte telomere length.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ADA clia kitPathways associated with ADA clia kit
 Reference Product  PubMed Publications
 PNP clia kit  >160 publications with ADA and PNP
 DCK clia kit  >10 publications with ADA and DCK
 ADK clia kit  >7 publications with ADA and ADK
 MYB clia kit  >6 publications with ADA and MYB
 NT5E clia kit  >4 publications with ADA and NT5E
 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Salvage Pathway antibodies  Purine Salvage Pathway Diagram
Organs/Tissues associated with ADA clia kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2552 publications with ADA and Blood
 Bone Antibodies  >337 publications with ADA and Bone
 Heart Antibodies  >295 publications with ADA and Heart
 Brain Antibodies  >288 publications with ADA and Brain
 Lung Antibodies  >241 publications with ADA and Lung
 Bone Marrow Antibodies  >232 publications with ADA and Bone Marrow
 Liver Antibodies  >227 publications with ADA and Liver
 Kidney Antibodies  >170 publications with ADA and Kidney
 Spleen Antibodies  >156 publications with ADA and Spleen
 Skin Antibodies  >152 publications with ADA and Skin
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