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AngI clia kit :: Human Angiotensin I (AngI) CLIA Kit

Scan QR to view Datasheet Catalog #    MBS2001191 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $440 +1 FREE 8GB USB
48-Strip-Wells  /  $655 +1 FREE 8GB USB
96-Strip-Wells  /  $900 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,705 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,680 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Angiotensin I (AngI), CLIA Kit

 Also Known As   

Angiotensin I (AngI) CLIA Kit

 Product Synonym Names    Ang-I; Angiotensin-1
 Product Gene Name   

AngI clia kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Angiotensin I (AngI).
No significant cross-reactivity or interference between Angiotensin I (AngI) and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
 Detection Range    7.81-2000pg/mL
 Sensitivity    The minimum detectable dose of this kit is typically less than 2.92pg/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I (AngI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I (AngI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 50uL standard or sample to each well.
    And then add 50uL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37 degree C;
3. Aspirate and wash 3 times;
4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
5. Aspirate and wash 5 times;
6. Add 100uL Substrate Solution. Incubate 10 minutes at 37 degree C;
7. Read RLU value immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of AngI clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for AngI clia kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Angiotensin I (AngI) and unlabeled Angiotensin I (AngI) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin I (AngI) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Angiotensin I (AngI) level in the sample or standard.
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 Typical Testing Data/Standard Curve (for reference only) of AngI clia kit    AngI clia kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2001191
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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