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ARHGEF25 clia kit :: Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) CLIA Kit

Scan QR to view Datasheet Catalog #    MBS2000519 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
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 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25), CLIA Kit

 Also Known As   

CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25)

 Product Gene Name   

ARHGEF25 clia kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 12; NC_000012.11 (58003963..58011028). Location: 12q13.3
 OMIM    610215
 3D Structure    ModBase 3D Structure for Q86VW2
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25)
No significant cross-reactivity or interference between Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) and analogues was observed.
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 Assay Type    Sandwich
 Samples    Tissue homogenates and other biological fluids
 Detection Range    0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
 Sensitivity    The minimum detectable dose of this kit is typically less than 0.073ng/mL
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC;
8. Read RLU value immediately.
 Organism Species    Homo sapiens (Human)
 Assay Length    4.5 hours
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our CLIA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ARHGEF25 clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ARHGEF25 clia kit

   The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) level in the sample or standard.
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 Typical Testing Data/Standard Curve (for reference only) of ARHGEF25 clia kit    ARHGEF25 clia kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2000519. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ARHGEF25. It may not necessarily be applicable to this product.
 NCBI GI #    150417975
 NCBI GeneID    115557
 NCBI Accession #    NP_891992.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_182947.3 [Other Products]
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 UniProt Primary Accession #    Q86VW2 [Other Products]
 UniProt Secondary Accession #    Q8WV84; Q96E63; A6NJH5; A9CQZ6; F8W7Z4 [Other Products]
 UniProt Related Accession #    Q86VW2 [Other Products]
 Molecular Weight    63,843 Da
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 NCBI Official Full Name    rho guanine nucleotide exchange factor 25 isoform 1
 NCBI Official Synonym Full Names    Rho guanine nucleotide exchange factor (GEF) 25
 NCBI Official Symbol    ARHGEF25 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    rho guanine nucleotide exchange factor 25; RAC/CDC42 exchange factor; RhoA/RAC/CDC42 exchange factor; rac/Cdc42/Rho exchange factor GEFT; guanine nucleotide exchange factor GEFT; rhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT
 UniProt Protein Name    Rho guanine nucleotide exchange factor 25
 UniProt Synonym Protein Names   
Guanine nucleotide exchange factor GEFT; Rac/Cdc42/Rho exchange factor GEFT; RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT; p63RhoGEF
 Protein Family    Rho guanine nucleotide exchange factor
 UniProt Gene Name    ARHGEF25 [Similar Products]
 UniProt Synonym Gene Names    GEFT [Similar Products]
 UniProt Entry Name    ARHGP_HUMAN
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 NCBI Summary for ARHGEF25    Rho GTPases alternate between an inactive GDP-bound state and an active GTP-bound state, and GEFs facilitate GDP/GTP exchange. This gene encodes a guanine nucleotide exchange factor (GEF) which interacts with Rho GTPases involved in contraction of vascular smooth muscles, regulation of responses to angiotensin II and lens cell differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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 UniProt Comments for ARHGEF25    GEFT: May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11- coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: sarcomere; myofibril; plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity
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 Research Articles on ARHGEF25    1. p63RhoGEF-mediated formation of a single polarized lamellipodium is required for chemotactic migration in breast carcinoma cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ARHGEF25 clia kitOrgans/Tissues associated with ARHGEF25 clia kit
 Products by Pathway  Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Regulation Of CDC42 Activity Pathway antibodies  Regulation Of CDC42 Activity Pathway Diagram
 Regulation Of RAC1 Activity Pathway antibodies  Regulation Of RAC1 Activity Pathway Diagram
 Regulation Of RhoA Activity Pathway antibodies  Regulation Of RhoA Activity Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with ARHGEF25 and Lung
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