View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Edinburgh Minimal Media Phosphate Free Culture Media Catalog # MBS652754 Unit / Price 100 g / $200 +1 FREE 8GB USB 500 g / $265 +1 FREE 8GB USB 2.5 kg / $840 +1 FREE 8GB USB 5x2.5 kg / $3,480 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Edinburgh Minimal Media Phosphate Free, Culture Media Popular Item Full Product Name Edinburgh Minimal Media Phosphate Free (EMMP) (Powder) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Purity/Purification Molecular Biology Grade pH 6.5 Solubility Colorless, clear, complete Appearance White, homogenous, free flowing powder. Directions per Liter Dissolve 29.328 grams per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Note: May also sterilize with 0.2um membrane filter. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving. Base Ammonium Chloride: 5.000Glucose: 20.000Sodium Acetate3H2O: 2.200 Salt Stock Calcium Chloride2H2O: 0.0147Magnesium Chloride6H2O: 1.050Potassium Chloride: 1.000Sodium Sulfate: 0.040 Vitamin Stock Biotin: 0.00001Inositol: 0.010Nicotinic Acid: 0.010Pantothenic AcidCa: 0.001 Mineral Stock Boric Acid: 0.0005Citric Acid: 0.001Copper Sulfate5H2O: 0.00004Ferrous Chloride: 0.0002Manganese Sulfate: 0.0004Potassium Iodide: 0.0001Molybdic Acid: 0.00004Zinc Sulfate7H2O: 0.0004 Total 29.328g/Lit Note The above formulation was derived from the following referenced formula Edinburgh Minimal Medium (EMM 2) 3g/L Potassium hydrogen phthalate (14.7mM)2.2g/L Sodium phosphate dibasic (15.5mM)5g/L Ammonium chloride (93.5mM)2% (w/v) Glucose (111mM)20ml/L Salts (50X stock)1ml/L Vitamins (1000X stock)0.1ml/L Minerals (10,000X stock) 50X Salts 52.5g/L Magnesium chloride6H2O (0.26M)0.735g/L Calcium chloride2H2O (4.99mM)50g/L Potassium chloride (0.67M)2g/L Sodium sulfate (14.1mM) 1000X Vitamins 1g/L Pantothenic acid (4.20mM)10g/L Nicotinic acid (81.2mM)10g/L Inositol (55.5mM)10mg/L Biotin (40.8uM) 10,000X Minerals 5g/L Boric acid (SO.9mM)4g/L Manganese sulfate (23.7mM) )4g/L Zinc sulfate7H20 (13.9mM) )2g/L Ferrous Chloride (7AOmM) )0.4g/L Molybdic acid (2A7mM) )1 g/L Potassium iodide (6.02mM) )0.4g/L Cupric sulfate.5H20 (1.60mM) )10g/L Citric acid (47.6mM) Preparation and Storage Store powdered media ar RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time. Other Notes Small volumes of Edinburgh Minimal Media Phosphate Free culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Edinburgh Minimal Media Phosphate Free culture media Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. Phosphate Free formulation is used for 32P-phosphate labeling.Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L sodium acetate (14.6mM). The medium is adjusted to pH 5.5. Product Categories/Family for Edinburgh Minimal Media Phosphate Free culture media Culture Media; Media-Yeast Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP