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Biotin elisa kit

Scan QR to view Datasheet Catalog #    MBS706024 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Biotin ELISA Kit

★Popular Item★
 Product Synonym Names    Biotin ELISA Kit; Biotin
 Product Gene Name   

Biotin elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS706024 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for E9A979
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 Specificity    This assay has high sensitivity and excellent specificity for detection of biotin. No significant cross-reactivity or interference between biotin and analogues was observed.
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 Samples    Serum, plasma from human, mouse and other species.
 Assay Type    Competitive
 Detection Range    12.5 pg/ml-200 pg/ml.
 Sensitivity    The minimum detectable dose of biotin is typically less than 4.986 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest biotin concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Biotin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Biotin purchase    MBS706024 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Biotin ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5 pg/ml-200 pg/ml in biological research samples containing Biotin, with an estimated sensitivity of The minimum detectable dose of biotin is typically less than 4.986 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest biotin concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS706024 kit is based on Biotin antibody-Biotin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Biotin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Biotin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma from human, mouse and other species. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Biotin elisa kit

   Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. The microplate was pre-coated with Avidin. Add Biotin (Standards or samples) to the well, and then add HRP-conjugated Biotin. A competitive inhibition reaction is launched between Biotin (Standards or samples) and HRP conjugated Biotin with the Avidin. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of Biotin bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of Biotin elisa kit    Biotin elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS706024. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Biotin. It may not necessarily be applicable to this product.
 NCBI GI #    320088538
 NCBI Accession #    CBY98296.1 [Other Products]
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 UniProt Primary Accession #    E9A979 [Other Products]
 Molecular Weight    35,396 Da
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 NCBI Official Full Name    biotin
 UniProt Protein Name    Biotin
 UniProt Synonym Protein Names   
Biotin
 UniProt Gene Name    birA [Similar Products]
 UniProt Synonym Gene Names    SENTW_4215 [Similar Products]
 UniProt Entry Name    E9A979_SALET
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Biotin elisa kitPathways associated with Biotin elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with Biotin and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with Biotin elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with Biotin and Bone
 Connective Tissue Antibodies  >4 publications with Biotin and Connective Tissue
 Heart Antibodies  >3 publications with Biotin and Heart
 Muscle Antibodies  >3 publications with Biotin and Muscle
 Brain Antibodies  >3 publications with Biotin and Brain
 Vascular Antibodies  >2 publications with Biotin and Vascular
 Embryonic Tissue Antibodies  >2 publications with Biotin and Embryonic Tissue
 Ear Antibodies  >1 publications with Biotin and Ear
 Kidney Antibodies  >1 publications with Biotin and Kidney
 Umbilical Cord Antibodies  >1 publications with Biotin and Umbilical Cord
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