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ARID5B elisa kit :: Bovine AT-rich interactive domain-containing protein 5B (ARID5B) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7247035 ARID5B elisa kit
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AT-rich interactive domain-containing protein 5B (ARID5B), ELISA Kit

 Also Known As   

Bovine AT-rich interactive domain-containing protein 5B (ARID5B) ELISA Kit

 Product Gene Name   

ARID5B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Bovine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ARID5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ARID5B purchase    MBS7247035 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AT-rich interactive domain-containing protein 5B (ARID5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ARID5B. The ELISA analytical biochemical technique of the MBS7247035 kit is based on ARID5B antibody-ARID5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ARID5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ARID5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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 Product Categories/Family for ARID5B elisa kit    Epigenetics and Nuclear Signaling
NCBI/Uniprot data below describe general gene information for ARID5B. It may not necessarily be applicable to this product.
 NCBI GI #    74136557
 NCBI GeneID    71371
 NCBI Accession #    NP_076087.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_023598.2 [Other Products]
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 UniProt Secondary Accession #    Q8C0E0; Q8K4G8; Q8K4L9; Q9CU78; Q9JIX4; E9Q523 [Other Products]
 UniProt Related Accession #    Q8BM75 [Other Products]
 Molecular Weight    31,400 Da
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 NCBI Official Full Name    AT-rich interactive domain-containing protein 5B
 NCBI Official Synonym Full Names    AT rich interactive domain 5B (MRF1-like)
 NCBI Official Symbol    Arid5b [Similar Products]
 NCBI Official Synonym Symbols   
Mrf2; Desrt; AI467247; Mrf2beta; Mrf2alpha; 4930580B11; 5430435G07Rik
[Similar Products]
 NCBI Protein Information    AT-rich interactive domain-containing protein 5B; MRF-2; ARID class DNA binding protein; modulator recognition factor 2; ARID domain-containing protein 5B; modulator recognition factor protein 2; AT rich interactive domain 5B (Mrf1 like); developmentally and sexually retarded with transient immune abnormalities protein
 UniProt Protein Name    AT-rich interactive domain-containing protein 5B
 UniProt Synonym Protein Names   
Developmentally and sexually retarded with transient immune abnormalities protein; Desrt; MRF1-like; Modulator recognition factor protein 2; MRF-2
 Protein Family    AT-rich interactive domain-containing protein
 UniProt Gene Name    Arid5b [Similar Products]
 UniProt Synonym Gene Names    Desrt; Mrf2; ARID domain-containing protein 5B; Desrt; MRF-2 [Similar Products]
 UniProt Entry Name    ARI5B_MOUSE
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 UniProt Comments for ARID5B    Mrf-2: Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. Defects in ARID5B may be a cause of susceptibility to coronary atherosclerosis in the Japanese population. Defects in ARID5B may be a cause of susceptibility to acute lymphoblastic leukemia (ALL). ALL is a subtype of acute leukemia, a cancer of the white blood cells. ALL is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphonodes. Belongs to the ARID5B family. 3 isoforms of the human protein are produced by alternative splicing.

Cellular Component: nucleus

Molecular Function: DNA binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; transcription, DNA-dependent; adrenal gland development; multicellular organism growth; male gonad development; platelet-derived growth factor receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; palate development; post-embryonic development; regulation of transcription from RNA polymerase II promoter; nitrogen compound metabolic process; regulation of transcription, DNA-dependent; skeletal morphogenesis; positive regulation of transcription factor activity; kidney development; muscle morphogenesis; cell development; female gonad development
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 Research Articles on ARID5B    1. knockdown of Mrf-2 increases the rate of fatty acid recycling in 3T3-L1-derived adipocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ARID5B elisa kitDiseases associated with ARID5B elisa kit
 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 HDMs Demethylate Histones Pathway antibodies  HDMs Demethylate Histones Pathway Diagram
 Disease Name  Pubmed Publications
 Precursor Cell Lymphoblastic Leukemia-Lymphoma Antibodies  >23 publications with ARID5B and Precursor Cell Lymphoblastic Leukemia-Lymphoma
 Arthritis, Rheumatoid Antibodies  >3 publications with ARID5B and Arthritis, Rheumatoid
 Cardiovascular Diseases Antibodies  >2 publications with ARID5B and Cardiovascular Diseases
 Heart Diseases Antibodies  >2 publications with ARID5B and Heart Diseases
 Graves Disease Antibodies  >2 publications with ARID5B and Graves Disease
 Uterine Neoplasms Antibodies  >1 publications with ARID5B and Uterine Neoplasms
 Breast Neoplasms Antibodies  >1 publications with ARID5B and Breast Neoplasms
 Weight Loss Antibodies  >1 publications with ARID5B and Weight Loss
 Inflammation Antibodies  >1 publications with ARID5B and Inflammation
Organs/Tissues associated with ARID5B elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with ARID5B and Blood
 Embryonic Tissue Antibodies  >3 publications with ARID5B and Embryonic Tissue
 Bone Marrow Antibodies  >1 publications with ARID5B and Bone Marrow
 Thyroid Antibodies  >1 publications with ARID5B and Thyroid
 Connective Tissue Antibodies  >1 publications with ARID5B and Connective Tissue
 Heart Antibodies  >1 publications with ARID5B and Heart
 Skin Antibodies  >1 publications with ARID5B and Skin
 Eye Antibodies  >1 publications with ARID5B and Eye
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