Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow ACTG2 rightarrow Bovine ACTG2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Estrogen Receptor Beta (ERb) Antibody
 • Anti thymocyte globulin (ATG) ELISA Kit
 • ARAF Antibody
 • High Mobility Group Box1 Protein (HMGB1) ELISA Kit
 • 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit
 • IFIT1 Antibody
 • G9a (EHMT2) Antibody
 • ARRbeta1 ELISA Kit
 • CD39L3 (ENTPD3) Antibody
 • AP1G1 Antibody
 • Cerebellin 1 (CBLN1) ELISA Kit
 • BMP-6 Antibody
 • CX3CR1 (NT) Antibody
 • Chromatin Modifying Protein 2A (CHMP2A) Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ACTG2 elisa kit :: Bovine Actin, gamma-enteric smooth muscle (ACTG2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7215539
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Actin, gamma-enteric smooth muscle (ACTG2), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Actin, gamma-enteric smooth muscle (ACTG2) ELISA Kit

 Product Gene Name   

ACTG2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    102545
Section Bar
 Species Reactivity    Bovine
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of ACTG2. No significant cross-reactivity or interference between ACTG2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTG2 and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ?g/mL.
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ACTG2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ACTG2 purchase    MBS7215539 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Actin, gamma-enteric smooth muscle (ACTG2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACTG2, with an estimated sensitivity of 0.1 ?g/mL. The ELISA analytical biochemical technique of the MBS7215539 kit is based on ACTG2 antibody-ACTG2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACTG2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACTG2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for ACTG2 elisa kit

   Intended Uses: This ACTG2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ACTG2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ACTG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACTG2 antibody and an ACTG2-HRP conjugate. The assay sample and buffer are incubated together with ACTG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTG2 concentration since ACTG2 from samples and ACTG2-HRP conjugate compete for the anti-ACTG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTG2 from the sample, fewer sites are left to bind ACTG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTG2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for ACTG2 elisa kit    Signal Transduction
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of ACTG2 elisa kit    ACTG2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS7215539. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ACTG2. It may not necessarily be applicable to this product.
 NCBI GI #    4501889
 NCBI GeneID    72
 NCBI Accession #    NP_001606.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001615.3 [Other Products]
Section Bar
 UniProt Secondary Accession #    P12718; Q504R1; Q6FI22; B2R7E7; B4E315; D6W5H8; E9PG30 [Other Products]
 UniProt Related Accession #    P63267 [Other Products]
 Molecular Weight    37,083 Da
Table BarTOPTable Bar
 NCBI Official Full Name    actin, gamma-enteric smooth muscle isoform 1
 NCBI Official Synonym Full Names    actin, gamma 2, smooth muscle, enteric
 NCBI Official Symbol    ACTG2 [Similar Products]
 NCBI Official Synonym Symbols   
ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
[Similar Products]
 NCBI Protein Information    actin, gamma-enteric smooth muscle; alpha-actin-3; actin-like protein
 UniProt Protein Name    Actin, gamma-enteric smooth muscle
 UniProt Synonym Protein Names   
Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin
 Protein Family    Actin
 UniProt Gene Name    ACTG2 [Similar Products]
 UniProt Synonym Gene Names    ACTA3; ACTL3; ACTSG [Similar Products]
 UniProt Entry Name    ACTH_HUMAN
Section Bar
 NCBI Summary for ACTG2    Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]
Section Bar
 UniProt Comments for ACTG2    ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular space; cytoskeleton; cytosol

Molecular Function: ATP binding

Biological Process: muscle contraction

Disease: Visceral Myopathy
Table BarTOPTable Bar
 Research Articles on ACTG2    1. Two novel mutations in the ACTG2 gene, p.R178L and p.R178C, have been identified in two unrelated children with congenital distended bladder, microcolon, and intestinal hypoperistalsis (MMIHS).
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ACTG2 elisa kitPathways associated with ACTG2 elisa kit
 Reference Product  PubMed Publications
 ACTA2 elisa kit  >2 publications with ACTG2 and ACTA2
 TAGLN elisa kit  >2 publications with ACTG2 and TAGLN
 CNN1 elisa kit  >1 publications with ACTG2 and CNN1
 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Smooth Muscle Contraction Pathway antibodies  Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
Diseases associated with ACTG2 elisa kitOrgans/Tissues associated with ACTG2 elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >3 publications with ACTG2 and Adenocarcinoma
 Liver Neoplasms Antibodies  >3 publications with ACTG2 and Liver Neoplasms
 Neoplasm Metastasis Antibodies  >3 publications with ACTG2 and Neoplasm Metastasis
 Liver Diseases Antibodies  >2 publications with ACTG2 and Liver Diseases
 Megacystis microcolon intestinal hypoperistalsis syndrome Antibodies  >2 publications with ACTG2 and Megacystis microcolon intestinal hypoperistalsis syndrome
 Anemia Antibodies  >1 publications with ACTG2 and Anemia
 Disease Models, Animal Antibodies  >1 publications with ACTG2 and Disease Models, Animal
 Carcinoma, Hepatocellular Antibodies  >1 publications with ACTG2 and Carcinoma, Hepatocellular
 Lung Neoplasms Antibodies  >1 publications with ACTG2 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with ACTG2 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >10 publications with ACTG2 and Muscle
 Lung Antibodies  >3 publications with ACTG2 and Lung
 Liver Antibodies  >3 publications with ACTG2 and Liver
 Intestine Antibodies  >3 publications with ACTG2 and Intestine
 Bladder Antibodies  >2 publications with ACTG2 and Bladder
 Connective Tissue Antibodies  >2 publications with ACTG2 and Connective Tissue
 Kidney Antibodies  >1 publications with ACTG2 and Kidney
 Pancreas Antibodies  >1 publications with ACTG2 and Pancreas
 Embryonic Tissue Antibodies  >1 publications with ACTG2 and Embryonic Tissue
 Testis Antibodies  >1 publications with ACTG2 and Testis
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions