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AGC elisa kit :: Bovine Aggrecan ELISA Kit

Scan QR to view Datasheet Catalog #    MBS735779
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aggrecan (AGC), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Aggrecan ELISA Kit

 Product Gene Name   

AGC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of AGC. No significant cross-reactivity or interference between AGC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGC and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100ng/ml
 Sensitivity    1.0ng/ml
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AGC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AGC purchase    MBS735779 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aggrecan (AGC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/ml in biological research samples containing AGC, with an estimated sensitivity of 1.0ng/ml. The ELISA analytical biochemical technique of the MBS735779 kit is based on AGC antibody-AGC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AGC elisa kit

   Intended Uses: This AGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine AGC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: AGC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGC antibody and an AGC-HRP conjugate. The assay sample and buffer are incubated together with AGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGC concentration since AGC from samples and AGC-HRP conjugate compete for the anti-AGC antibody binding site. Since the number of sites is limited, as more sites are occupied by AGC from the sample, fewer sites are left to bind AGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for AGC elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of AGC elisa kit    AGC elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS735779. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AGC. It may not necessarily be applicable to this product.
 NCBI GI #    11990616
 NCBI GeneID    58968
 NCBI Accession #    NP_071526.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022190.1 [Other Products]
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 UniProt Related Accession #    P07897 [Other Products]
 Molecular Weight    225,171 Da
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 NCBI Official Full Name    aggrecan core protein
 NCBI Official Synonym Full Names    aggrecan
 NCBI Official Symbol    Acan [Similar Products]
 NCBI Official Synonym Symbols   
Agc; Agc1
[Similar Products]
 NCBI Protein Information    aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein; aggrecan, structural proteoglycan of cartilage
 UniProt Protein Name    Aggrecan core protein
 UniProt Synonym Protein Names   
Cartilage-specific proteoglycan core protein; CSPCP
 Protein Family    Aggrecan core protein
 UniProt Gene Name    Acan [Similar Products]
 UniProt Synonym Gene Names    Agc; Agc1; CSPCP [Similar Products]
 UniProt Entry Name    PGCA_RAT
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 NCBI Summary for AGC    an extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006]
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 UniProt Comments for AGC    This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
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 Research Articles on AGC    1. These results support a role for MafB as a regulator of chondrocyte gene expression and matrix formation via control of aggrecan, MMP3 and MMP13 expression, and indicate an important role for RA in the regulation of MafB
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AGC elisa kitPathways associated with AGC elisa kit
 Reference Product  PubMed Publications
 Sox9 elisa kit  >61 publications with AGC and Sox9
 Adamts5 elisa kit  >13 publications with AGC and Adamts5
 Adamts4 elisa kit  >9 publications with AGC and Adamts4
 Products by Pathway  Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
Diseases associated with AGC elisa kitOrgans/Tissues associated with AGC elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >8 publications with AGC and Necrosis
 Pain Antibodies  >7 publications with AGC and Pain
 Inflammation Antibodies  >6 publications with AGC and Inflammation
 Arthritis, Rheumatoid Antibodies  >4 publications with AGC and Arthritis, Rheumatoid
 Immune System Diseases Antibodies  >3 publications with AGC and Immune System Diseases
 Nervous System Diseases Antibodies  >3 publications with AGC and Nervous System Diseases
 Weight Loss Antibodies  >2 publications with AGC and Weight Loss
 Carcinoma, Squamous Cell Antibodies  >2 publications with AGC and Carcinoma, Squamous Cell
 Hyperplasia Antibodies  >1 publications with AGC and Hyperplasia
 Fibrosis Antibodies  >1 publications with AGC and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >26 publications with AGC and Connective Tissue
 Brain Antibodies  >13 publications with AGC and Brain
 Embryonic Tissue Antibodies  >6 publications with AGC and Embryonic Tissue
 Lung Antibodies  >1 publications with AGC and Lung
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