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ALD elisa kit :: Bovine Aldosterone ELISA Kit

Scan QR to view Datasheet Catalog #    MBS019777 ALD elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aldosterone, ELISA Kit

★Popular Item★
 Also Known As   

Bovine Aldosterone ELISA Kit

 Product Gene Name   

ALD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    300100
 3D Structure    ModBase 3D Structure for P33897
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 Species Reactivity    Bovine
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 Specificity    No significant cross-reactivity or interference between Bovine ALD and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    31.2 pg/ml - 1000 pg/ml.
 Sensitivity    5.0 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ALD concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine ALD antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ALD and ALD antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ALD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALD purchase    MBS019777 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aldosterone, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 pg/ml - 1000 pg/ml in biological research samples containing ALD, with an estimated sensitivity of 5.0 pg/ml. The ELISA analytical biochemical technique of the MBS019777 kit is based on ALD antibody-ALD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ALD elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ALD concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine ALD antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ALD and ALD antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ALD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS019777. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALD. It may not necessarily be applicable to this product.
 NCBI GI #    67476960
 NCBI GeneID    215
 NCBI Accession #    P33897.2 [Other Products]
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 UniProt Primary Accession #    P33897 [Other Products]
 UniProt Secondary Accession #    Q6GTZ2 [Other Products]
 UniProt Related Accession #    P33897 [Other Products]
 Molecular Weight    82,937 Da
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 NCBI Official Full Name    ATP-binding cassette sub-family D member 1
 NCBI Official Synonym Full Names    ATP-binding cassette, sub-family D (ALD), member 1
 NCBI Official Symbol    ABCD1 [Similar Products]
 NCBI Official Synonym Symbols   
ALD; AMN; ALDP; ABC42
[Similar Products]
 NCBI Protein Information    ATP-binding cassette sub-family D member 1; adrenoleukodystrophy protein
 UniProt Protein Name    ATP-binding cassette sub-family D member 1
 UniProt Synonym Protein Names   
Adrenoleukodystrophy protein
 Protein Family    Alanine dehydrogenase
 UniProt Gene Name    ABCD1 [Similar Products]
 UniProt Synonym Gene Names    ALD; ALDP [Similar Products]
 UniProt Entry Name    ABCD1_HUMAN
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 NCBI Summary for ALD    The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein is likely involved in the peroxisomal transport or catabolism of very long chain fatty acids. Defects in this gene have been identified as the underlying cause of adrenoleukodystrophy, an X-chromosome recessively inherited demyelinating disorder of the nervous system. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ALD    ABCD1: Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity. Defects in ABCD1 are the cause of adrenoleukodystrophy X- linked (X-ALD). X-ALD is a peroxisomal metabolic disorder characterized by progressive multifocal demyelination of the central nervous system and by peripheral adrenal insufficiency (Addison disease). It results in mental deterioration, corticospinal tract dysfunction, and cortical blindness. Different clinical manifestations exist like: cerebral childhood ALD (CALD), adult cerebral ALD (ACALD), adrenomyeloneuropathy (AMN) and 'Addison disease only' (ADO) phenotype. The promoter region of ABCD1 is deleted in the chromosome Xq28 deletion syndrome which involves ABCD1 and the neighboring gene BCAP31. Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.

Protein type: Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Hydrolase; Transporter

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: peroxisomal membrane; integral to peroxisomal membrane; mitochondrion; membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; peroxisomal fatty acyl CoA transporter activity; enzyme binding; ATPase activity, coupled to transmembrane movement of substances; transporter activity; ATPase activity; ATP binding

Biological Process: fatty acid beta-oxidation using acyl-CoA oxidase; peroxisomal membrane transport; fatty acid beta-oxidation; peroxisomal long-chain fatty acid import; very-long-chain fatty acid catabolic process; peroxisome organization and biogenesis; unsaturated fatty acid metabolic process; linoleic acid metabolic process; cellular lipid metabolic process; transmembrane transport; long-chain fatty acid catabolic process

Disease: Adrenoleukodystrophy
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 Research Articles on ALD    1. X-inactivation pattern of the ABCD1 gene is associated with symptomatic status in female X-linked adrenoleukodystrophy carriers.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ALD elisa kitPathways associated with ALD elisa kit
 Reference Product  PubMed Publications
 ABCD2 elisa kit  >59 publications with ALD and ABCD2
 ABCD3 elisa kit  >39 publications with ALD and ABCD3
 BCAP31 elisa kit  >4 publications with ALD and BCAP31
 HSD17B4 elisa kit  >3 publications with ALD and HSD17B4
 PEX19 elisa kit  >3 publications with ALD and PEX19
 PLXNB3 elisa kit  >2 publications with ALD and PLXNB3
 ACLY elisa kit  >1 publications with ALD and ACLY
 Products by Pathway  Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 ABCA Transporters In Lipid Homeostasis Pathway antibodies  ABCA Transporters In Lipid Homeostasis Pathway Diagram
 Beta-oxidation Of Very Long Chain Fatty Acids Pathway antibodies  Beta-oxidation Of Very Long Chain Fatty Acids Pathway Diagram
 Linoleic Acid (LA) Metabolism Pathway antibodies  Linoleic Acid (LA) Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Peroxisomal Lipid Metabolism Pathway antibodies  Peroxisomal Lipid Metabolism Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
Diseases associated with ALD elisa kitOrgans/Tissues associated with ALD elisa kit
 Disease Name  Pubmed Publications
 Adrenoleukodystrophy Antibodies  >315 publications with ALD and Adrenoleukodystrophy
 Nervous System Diseases Antibodies  >272 publications with ALD and Nervous System Diseases
 Fatty Liver Antibodies  >19 publications with ALD and Fatty Liver
 Inflammation Antibodies  >18 publications with ALD and Inflammation
 Liver Diseases Antibodies  >7 publications with ALD and Liver Diseases
 Carcinoma Antibodies  >6 publications with ALD and Carcinoma
 Aneuploidy Antibodies  >5 publications with ALD and Aneuploidy
 Kidney Diseases Antibodies  >5 publications with ALD and Kidney Diseases
 Carcinoma, Hepatocellular Antibodies  >3 publications with ALD and Carcinoma, Hepatocellular
 Cognition Disorders Antibodies  >3 publications with ALD and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >102 publications with ALD and Brain
 Blood Antibodies  >62 publications with ALD and Blood
 Liver Antibodies  >30 publications with ALD and Liver
 Adrenal Gland Antibodies  >28 publications with ALD and Adrenal Gland
 Skin Antibodies  >25 publications with ALD and Skin
 Nerve Antibodies  >19 publications with ALD and Nerve
 Bone Antibodies  >14 publications with ALD and Bone
 Testis Antibodies  >13 publications with ALD and Testis
 Kidney Antibodies  >11 publications with ALD and Kidney
 Muscle Antibodies  >5 publications with ALD and Muscle
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