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APO-B100 elisa kit :: Bovine Apolipoprotein B100 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS041325 APO-B100 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apolipoprotein B100 (APO-B100), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Apolipoprotein B100 ELISA Kit

 Product Gene Name   

APO-B100 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 2; NC_000002.11 (21224301..21266945, complement). Location: 2p24-p23
 OMIM    107730
 3D Structure    ModBase 3D Structure for P04114
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 Species Reactivity    Bovine
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 Specificity    No significant cross-reactivity or interference between Bovine APO-B100 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    62.5 mug/ml - 2000 mug/ml.
 Sensitivity    10 mug/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated APO-B100 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine APO-B100 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APO-B100 and APO-B100 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APO-B100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of APO-B100 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for APO-B100 purchase    MBS041325 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein B100 (APO-B100) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 mug/ml - 2000 mug/ml in biological research samples containing APO-B100, with an estimated sensitivity of 10 mug/ml. The ELISA analytical biochemical technique of the MBS041325 kit is based on APO-B100 antibody-APO-B100 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APO-B100 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APO-B100. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for APO-B100 elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated APO-B100 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine APO-B100 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APO-B100 and APO-B100 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APO-B100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS041325. Click to request current manual
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NCBI/Uniprot data below describe general gene information for APO-B100. It may not necessarily be applicable to this product.
 NCBI GI #    28780
 NCBI GeneID    338
 NCBI Accession #    CAA28420.1 [Other Products]
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 UniProt Primary Accession #    P04114 [Other Products]
 UniProt Secondary Accession #    O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8 [Other Products]
 UniProt Related Accession #    P04114 [Other Products]
 Molecular Weight    515,605 Da
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 NCBI Official Full Name    apo-B100
 NCBI Official Synonym Full Names    apolipoprotein B
 NCBI Official Symbol    APOB [Similar Products]
 NCBI Official Synonym Symbols   
FLDB; LDLCQ4
[Similar Products]
 NCBI Protein Information    apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
 UniProt Protein Name    Apolipoprotein B-100
 UniProt Gene Name    APOB [Similar Products]
 UniProt Synonym Gene Names    Apo B-100; Apo B-48 [Similar Products]
 UniProt Entry Name    APOB_HUMAN
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 NCBI Summary for APO-B100    This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
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 UniProt Comments for APO-B100    APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Secreted, signal peptide; Secreted; Carrier

Chromosomal Location of Human Ortholog: 2p24-p23

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol; actin cytoskeleton; chylomicron; cell soma; cytoplasm; plasma membrane; endosome membrane

Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding

Biological Process: phototransduction, visible light; lipoprotein catabolic process; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; cholesterol metabolic process; nervous system development; receptor-mediated endocytosis; cholesterol transport; in utero embryonic development; response to virus; regulation of cholesterol biosynthetic process; lipoprotein metabolic process; cholesterol efflux; sperm motility; cholesterol homeostasis; fertilization; lipoprotein transport; lipoprotein biosynthetic process; artery morphogenesis; spermatogenesis; blood coagulation; triacylglycerol mobilization; leukocyte migration

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1
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 Research Articles on APO-B100    1. ApoB levels were associated with the PON1 "R" allele in Asian Indian women with coronary artery disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with APO-B100 elisa kitDiseases associated with APO-B100 elisa kit
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 LDL-mediated Lipid Transport Pathway antibodies  LDL-mediated Lipid Transport Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3228 publications with APO-B100 and Cardiovascular Diseases
 Dyslipidemias Antibodies  >2829 publications with APO-B100 and Dyslipidemias
 Atherosclerosis Antibodies  >2420 publications with APO-B100 and Atherosclerosis
 Hyperlipidemias Antibodies  >2366 publications with APO-B100 and Hyperlipidemias
 Coronary Disease Antibodies  >1784 publications with APO-B100 and Coronary Disease
 Heart Diseases Antibodies  >1537 publications with APO-B100 and Heart Diseases
 Hypercholesterolemia Antibodies  >1411 publications with APO-B100 and Hypercholesterolemia
 Hyperlipoproteinemia Type II Antibodies  >689 publications with APO-B100 and Hyperlipoproteinemia Type II
 HYPERCHOLESTEROLEMIA, AUTOSOMAL DOMINANT, TYPE B Antibodies  >689 publications with APO-B100 and HYPERCHOLESTEROLEMIA, AUTOSOMAL DOMINANT, TYPE B
 Insulin Resistance Antibodies  >635 publications with APO-B100 and Insulin Resistance
Organs/Tissues associated with APO-B100 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7694 publications with APO-B100 and Blood
 Liver Antibodies  >2129 publications with APO-B100 and Liver
 Heart Antibodies  >1382 publications with APO-B100 and Heart
 Intestine Antibodies  >562 publications with APO-B100 and Intestine
 Kidney Antibodies  >415 publications with APO-B100 and Kidney
 Muscle Antibodies  >301 publications with APO-B100 and Muscle
 Brain Antibodies  >133 publications with APO-B100 and Brain
 Lung Antibodies  >114 publications with APO-B100 and Lung
 Embryonic Tissue Antibodies  >55 publications with APO-B100 and Embryonic Tissue
 Spleen Antibodies  >55 publications with APO-B100 and Spleen
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