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BCDE elisa kit :: Bovine B cell differentiation factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS744410
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

B cell differentiation factor (BCDE), ELISA Kit

 Also Known As   

Bovine B cell differentiation factor ELISA Kit

 Product Gene Name   

BCDE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    147850
 3D Structure    ModBase 3D Structure for P05113
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 Species Reactivity    Bovine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
 Intended Uses    This BCDE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine BCDE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BCDE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BCDE purchase    MBS744410 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the B cell differentiation factor (BCDE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BCDE, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS744410 kit is based on BCDE antibody-BCDE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BCDE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BCDE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for BCDE elisa kit

   Principle of the Assay: BCDE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCDE antibody and an BCDE-HRP conjugate. The assay sample and buffer are incubated together with BCDE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCDE concentration since BCDE from samples and BCDE-HRP conjugate compete for the anti-BCDE antibody binding site. Since the number of sites is limited, as more sites are occupied by BCDE from the sample, fewer sites are left to bind BCDE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCDE concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for BCDE elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of BCDE elisa kit    BCDE elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS744410. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BCDE. It may not necessarily be applicable to this product.
 NCBI GI #    29393
 NCBI GeneID    3567
 NCBI Accession #    CAA31211.1 [Other Products]
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 UniProt Primary Accession #    P05113 [Other Products]
 UniProt Secondary Accession #    Q13840 [Other Products]
 UniProt Related Accession #    P05113 [Other Products]
 Molecular Weight    15,238 Da
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 NCBI Official Full Name    B cell differentiation factor I
 NCBI Official Synonym Full Names    interleukin 5
 NCBI Official Symbol    IL5 [Similar Products]
 NCBI Official Synonym Symbols   
EDF; TRF; IL-5
[Similar Products]
 NCBI Protein Information    interleukin-5; T-cell replacing factor; B-cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil
 UniProt Protein Name    Interleukin-5
 UniProt Synonym Protein Names   
B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
 UniProt Gene Name    IL5 [Similar Products]
 UniProt Synonym Gene Names    IL-5; TRF [Similar Products]
 UniProt Entry Name    IL5_HUMAN
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 NCBI Summary for BCDE    This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
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 UniProt Comments for BCDE    IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-5 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of transcription factor activity; immune response; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation
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 Research Articles on BCDE    1. 11beta-HSD2 expression was decreased in allergic nasal mucosa. In cultured epithelial cells treated with IL-4, IL-5, IL-13, and IL-17A, 11beta-HSD1 expression and activity increased in parallel with the expression levels of CYP11B1 and cortisol
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BCDE elisa kitPathways associated with BCDE elisa kit
 Reference Product  PubMed Publications
 IL4 elisa kit  >210 publications with BCDE and IL4
 IL2 elisa kit  >100 publications with BCDE and IL2
 IL3 elisa kit  >50 publications with BCDE and IL3
 JUN elisa kit  >36 publications with BCDE and JUN
 IL9 elisa kit  >24 publications with BCDE and IL9
 IL5RA elisa kit  >24 publications with BCDE and IL5RA
 CSF2RB elisa kit  >5 publications with BCDE and CSF2RB
 SDCBP elisa kit  >1 publications with BCDE and SDCBP
 FOS elisa kit  >1 publications with BCDE and FOS
 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
Diseases associated with BCDE elisa kitOrgans/Tissues associated with BCDE elisa kit
 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >124 publications with BCDE and Hypersensitivity
 Asthma Antibodies  >107 publications with BCDE and Asthma
 Inflammation Antibodies  >104 publications with BCDE and Inflammation
 Respiratory Hypersensitivity Antibodies  >91 publications with BCDE and Respiratory Hypersensitivity
 Lung Diseases Antibodies  >87 publications with BCDE and Lung Diseases
 Eosinophilia Antibodies  >66 publications with BCDE and Eosinophilia
 Necrosis Antibodies  >57 publications with BCDE and Necrosis
 Fibrosis Antibodies  >12 publications with BCDE and Fibrosis
 Bronchial Hyperreactivity Antibodies  >12 publications with BCDE and Bronchial Hyperreactivity
 Hyperplasia Antibodies  >10 publications with BCDE and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >7 publications with BCDE and Thymus
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