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DEFB4 elisa kit :: Bovine Beta-defensin 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS288127
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $560 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,445 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Beta-defensin 4 (DEFB4), ELISA Kit

 Also Known As   

Bovine Beta-defensin 4 ELISA Kit

 Product Gene Name   

DEFB4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P46162
 Species Reactivity    Bovine
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 Specificity    This assay recognizes recombinant and natural bovine Beta-defensin 4. No significant cross-reactivity or interference was observed.
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 Samples    Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
 Detection Range    0.31 ng/ml -20.0 ng/ml.
 Intended Uses    This immunoassay kit allows for the in vitro quantitative determination of bovine Beta-defensin 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DEFB4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFB4 purchase    MBS288127 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Beta-defensin 4 (DEFB4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.31 ng/ml -20.0 ng/ml in biological research samples containing DEFB4. The ELISA analytical biochemical technique of the MBS288127 kit is based on DEFB4 antibody-DEFB4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFB4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFB4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DEFB4 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-defensin 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Beta-defensin 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Beta-defensin 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Beta-defensin 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of DEFB4 elisa kit   
DEFB4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS288127. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DEFB4. It may not necessarily be applicable to this product.
 NCBI GI #    28849941
 NCBI GeneID    286836
 NCBI Accession #    NP_777200.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_174775.1 [Other Products]
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 UniProt Primary Accession #    P46162 [Other Products]
 UniProt Related Accession #    P46162 [Other Products]
 Molecular Weight    7,161 Da
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 NCBI Official Full Name    beta-defensin 4
 NCBI Official Symbol    DEFB4A [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    beta-defensin 4
 UniProt Protein Name    Beta-defensin 4
 UniProt Synonym Protein Names   
 Protein Family    Beta-defensin
 UniProt Gene Name    DEFB4 [Similar Products]
 UniProt Synonym Gene Names    BNBD4 [Similar Products]
 UniProt Entry Name    DEFB4_BOVIN
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 UniProt Comments for DEFB4    Has bactericidal activity. Active against E.coli ML35 and S.aureus 502A.
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 Research Articles on DEFB4    1. Recombinant mature bovine neutrophil beta-defensin 4 inhibits mycobacterial growth and controls intracellular survival of mycobacteria.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DEFB4 elisa kitDiseases associated with DEFB4 elisa kit
 Reference Product  PubMed Publications
 DEFB2 elisa kit  >10 publications with DEFB4 and DEFB2
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >128 publications with DEFB4 and Inflammation
 Necrosis Antibodies  >82 publications with DEFB4 and Necrosis
 Lung Diseases Antibodies  >48 publications with DEFB4 and Lung Diseases
 Carcinoma, Squamous Cell Antibodies  >21 publications with DEFB4 and Carcinoma, Squamous Cell
 Inflammatory Bowel Diseases Antibodies  >21 publications with DEFB4 and Inflammatory Bowel Diseases
 Cardiovascular Diseases Antibodies  >12 publications with DEFB4 and Cardiovascular Diseases
 Premature Birth Antibodies  >4 publications with DEFB4 and Premature Birth
 Skin Neoplasms Antibodies  >4 publications with DEFB4 and Skin Neoplasms
 Pulmonary Fibrosis Antibodies  >4 publications with DEFB4 and Pulmonary Fibrosis
 Lung Injury Antibodies  >4 publications with DEFB4 and Lung Injury
Organs/Tissues associated with DEFB4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >40 publications with DEFB4 and Intestine
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