Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow DDIT3 rightarrow Bovine DDIT3  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • EloA ELISA Kit
 • Factor V (F5) Antibody
 • Glutathione S Transferase Pi (GSTp) ELISA Kit
 • HA (H1N1) (A-California-04-2009) Recombinant Protein
 • Tumor necrosis factor receptor superfamily member 1B protein... Recombinant Protein
 • Influenza-A Hemagglutinin H3N2 Antibody
 • ADAM15 Antibody
 • Epiregulin (EREG) ELISA Kit
 • Epidermal Growth Factor (EGF) Antibody
 • Kanamycin Antibody
 • cystatin C (CST3) ELISA Kit
 • Connexin-45 (Cx45) Antibody
 • Vascular cell adhesion protein 1 (VCAM1) ELISA Kit
 • dickkopf homolog 1 (Xenopus laevis) (DKK1) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

DDIT3 elisa kit :: Bovine DNA Damage Inducible Transcript 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS746415
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DNA Damage Inducible Transcript 3 (DDIT3), ELISA Kit

 Also Known As   

Bovine DNA Damage Inducible Transcript 3 ELISA Kit

 Product Gene Name   

DDIT3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 613488
Section Bar
 Species Reactivity    Bovine
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Intended Uses    This DDIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DDIT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications
 !Intended Uses    This DDIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DDIT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DDIT3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DDIT3 purchase    MBS746415 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA Damage Inducible Transcript 3 (DDIT3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DDIT3. The ELISA analytical biochemical technique of the MBS746415 kit is based on DDIT3 antibody-DDIT3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DDIT3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DDIT3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for DDIT3 elisa kit

   Principle of the Assay: DDIT3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DDIT3. Standards or samples are then added to the microtiter plate wells and DDIT3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DDIT3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DDIT3 are added to each well to "sandwich" the DDIT3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DDIT3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDIT3 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for DDIT3 elisa kit    Cell Biology
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of DDIT3 elisa kit    DDIT3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS746415. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for DDIT3. It may not necessarily be applicable to this product.
 NCBI GI #    304282234
 NCBI GeneID    1649
 NCBI Accession #    NP_001181986.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001195057.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    F8VS99 [Other Products]
 UniProt Related Accession #    P35638 [Other Products]
 Molecular Weight    21,700 Da
Table BarTOPTable Bar
 NCBI Official Full Name    DNA damage-inducible transcript 3 protein isoform 2
 NCBI Official Synonym Full Names    DNA-damage-inducible transcript 3
 NCBI Official Symbol    DDIT3 [Similar Products]
 NCBI Official Synonym Symbols   
CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
[Similar Products]
 NCBI Protein Information    DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153
 UniProt Protein Name    DNA damage-inducible transcript 3 protein
 UniProt Synonym Protein Names   
C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153
 Protein Family    DNA damage-inducible transcript 3 protein
 UniProt Gene Name    DDIT3 [Similar Products]
 UniProt Synonym Gene Names    CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10 [Similar Products]
 UniProt Entry Name    DDIT3_HUMAN
Section Bar
 NCBI Summary for DDIT3    This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
Section Bar
 UniProt Comments for DDIT3    CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Autophagy

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: nucleoplasm; late endosome; cytosol; nucleus

Molecular Function: protein binding; leucine zipper domain binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription in response to stress; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; unfolded protein response; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; positive regulation of interleukin-8 production; response to starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; cell redox homeostasis; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation; cell cycle arrest; inhibition of CREB transcription factor; response to DNA damage stimulus; ER overload response

Disease: Myxoid Liposarcoma
Table BarTOPTable Bar
 Research Articles on DDIT3    1. They implicate CHOP as a common contributing factor in the development of HCC in a variety of chronic liver diseases.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with DDIT3 elisa kitPathways associated with DDIT3 elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >257 publications with DDIT3 and JUN
 ATF4 elisa kit  >247 publications with DDIT3 and ATF4
 ATF6 elisa kit  >191 publications with DDIT3 and ATF6
 XBP1 elisa kit  >141 publications with DDIT3 and XBP1
 ATF3 elisa kit  >60 publications with DDIT3 and ATF3
 BCL2 elisa kit  >25 publications with DDIT3 and BCL2
 CEBPB elisa kit  >8 publications with DDIT3 and CEBPB
 JDP2 elisa kit  >2 publications with DDIT3 and JDP2
 MAPK14 elisa kit  >1 publications with DDIT3 and MAPK14
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 ATF6-alpha Activates Chaperone Genes Pathway antibodies  ATF6-alpha Activates Chaperone Genes Pathway Diagram
 ATF6-alpha Activates Chaperones Pathway antibodies  ATF6-alpha Activates Chaperones Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram
Diseases associated with DDIT3 elisa kitOrgans/Tissues associated with DDIT3 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >162 publications with DDIT3 and Nervous System Diseases
 Disease Models, Animal Antibodies  >146 publications with DDIT3 and Disease Models, Animal
 Necrosis Antibodies  >137 publications with DDIT3 and Necrosis
 Inflammation Antibodies  >118 publications with DDIT3 and Inflammation
 Brain Diseases Antibodies  >115 publications with DDIT3 and Brain Diseases
 Liposarcoma, Myxoid Antibodies  >114 publications with DDIT3 and Liposarcoma, Myxoid
 Liver Diseases Antibodies  >107 publications with DDIT3 and Liver Diseases
 Liver Neoplasms Antibodies  >59 publications with DDIT3 and Liver Neoplasms
 Breast Neoplasms Antibodies  >55 publications with DDIT3 and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >51 publications with DDIT3 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >190 publications with DDIT3 and Brain
 Liver Antibodies  >172 publications with DDIT3 and Liver
 Blood Antibodies  >162 publications with DDIT3 and Blood
 Muscle Antibodies  >113 publications with DDIT3 and Muscle
 Lung Antibodies  >103 publications with DDIT3 and Lung
 Pancreas Antibodies  >97 publications with DDIT3 and Pancreas
 Kidney Antibodies  >97 publications with DDIT3 and Kidney
 Vascular Antibodies  >87 publications with DDIT3 and Vascular
 Heart Antibodies  >81 publications with DDIT3 and Heart
 Bone Antibodies  >59 publications with DDIT3 and Bone
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions