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FPA elisa kit :: Bovine Fibrinopeptide A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS735038
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Fibrinopeptide A (FPA), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Fibrinopeptide A ELISA Kit

 Product Gene Name   

FPA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene+202400
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 Species Reactivity    Bovine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FPA purchase    MBS735038 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fibrinopeptide A (FPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FPA, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS735038 kit is based on FPA antibody-FPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FPA elisa kit

   Intended Uses: This FPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FPA antibody and an FPA-HRP conjugate. The assay sample and buffer are incubated together with FPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FPA concentration since FPA from samples and FPA-HRP conjugate compete for the anti-FPA antibody binding site. Since the number of sites is limited, as more sites are occupied by FPA from the sample, fewer sites are left to bind FPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FPA concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for FPA elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of FPA elisa kit    FPA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS735038. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FPA. It may not necessarily be applicable to this product.
 NCBI GI #    11761629
 NCBI GeneID    2243
 NCBI Accession #    NP_068657.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021871.2 [Other Products]
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 UniProt Secondary Accession #    Q4QQH7; Q9BX62; Q9UCH2; A8K3E4; D3DP14; D3DP15 [Other Products]
 UniProt Related Accession #    P02671 [Other Products]
 Molecular Weight    69,757 Da
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 NCBI Official Full Name    fibrinogen alpha chain isoform alpha preproprotein
 NCBI Official Synonym Full Names    fibrinogen alpha chain
 NCBI Official Symbol    FGA [Similar Products]
 NCBI Official Synonym Symbols   
Fib2
[Similar Products]
 NCBI Protein Information    fibrinogen alpha chain; fibrinogen, A alpha polypeptide
 UniProt Protein Name    Fibrinogen alpha chain
 Protein Family    Flowering time control protein
 UniProt Gene Name    FGA [Similar Products]
 UniProt Entry Name    FIBA_HUMAN
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 NCBI Summary for FPA    The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FPA    FGA: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGA are a cause of congenital afibrinogenemia (CAFBN). This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha- dysfibrinogenemias. Defects in FGA are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: extracellular space; cell surface; fibrinogen complex; plasma membrane; extracellular region; cell cortex; vesicle; external side of plasma membrane

Molecular Function: protein binding, bridging; protein binding; cell adhesion molecule binding; structural molecule activity; receptor binding

Biological Process: protein polymerization; platelet activation; extracellular matrix organization and biogenesis; positive regulation of heterotypic cell-cell adhesion; cell-matrix adhesion; signal transduction; cellular protein complex assembly; platelet degranulation; positive regulation of protein secretion; positive regulation of vasoconstriction; innate immune response; blood coagulation; response to calcium ion; positive regulation of exocytosis

Disease: Amyloidosis, Familial Visceral; Afibrinogenemia, Congenital; Dysfibrinogenemia, Congenital
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 Research Articles on FPA    1. role in regulation of clot formation and fibrinolysis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FPA elisa kitPathways associated with FPA elisa kit
 Reference Product  PubMed Publications
 FGB elisa kit  >82 publications with FPA and FGB
 FGG elisa kit  >74 publications with FPA and FGG
 F13A1 elisa kit  >10 publications with FPA and F13A1
 F13B elisa kit  >8 publications with FPA and F13B
 ITGA2B elisa kit  >2 publications with FPA and ITGA2B
 ITGB3 elisa kit  >2 publications with FPA and ITGB3
 APOA1 elisa kit  >1 publications with FPA and APOA1
 Products by Pathway  Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 GRB2:SOS Provides Linkage To MAPK Signaling For Integrins Pathway antibodies  GRB2:SOS Provides Linkage To MAPK Signaling For Integrins Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with FPA elisa kitOrgans/Tissues associated with FPA elisa kit
 Disease Name  Pubmed Publications
 Afibrinogenemia Antibodies  >55 publications with FPA and Afibrinogenemia
 Afibrinogenemia congenital Antibodies  >54 publications with FPA and Afibrinogenemia congenital
 Cardiovascular Diseases Antibodies  >52 publications with FPA and Cardiovascular Diseases
 Brain Diseases Antibodies  >35 publications with FPA and Brain Diseases
 Thrombosis Antibodies  >20 publications with FPA and Thrombosis
 Weight Gain Antibodies  >16 publications with FPA and Weight Gain
 Hemorrhage Antibodies  >13 publications with FPA and Hemorrhage
 Inflammation Antibodies  >12 publications with FPA and Inflammation
 Hypertension Antibodies  >10 publications with FPA and Hypertension
 Necrosis Antibodies  >10 publications with FPA and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >188 publications with FPA and Blood
 Brain Antibodies  >28 publications with FPA and Brain
 Liver Antibodies  >18 publications with FPA and Liver
 Lung Antibodies  >17 publications with FPA and Lung
 Heart Antibodies  >17 publications with FPA and Heart
 Muscle Antibodies  >8 publications with FPA and Muscle
 Kidney Antibodies  >8 publications with FPA and Kidney
 Uterus Antibodies  >5 publications with FPA and Uterus
 Embryonic Tissue Antibodies  >5 publications with FPA and Embryonic Tissue
 Spleen Antibodies  >3 publications with FPA and Spleen
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