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Lpro elisa kit :: Bovine Foot and Mouth Disease Virus (FMDV) antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704455 Lpro elisa kit
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Foot and Mouth Disease Virus (FMDV) antibody (Lpro), ELISA Kit

 Also Known As   

Bovine Foot and Mouth Disease Virus, FMDV antibody ELISA Kit

 Product Synonym Names    Bovine Foot and Mouth Disease Virus (FMDV) antibody ELISA Kit; Foot and Mouth Disease Virus (FMDV) antibody
 Product Gene Name   

Lpro elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for P03308
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of bovine FMDV antibody. No significant cross-reactivity or interference between bovine FMDV antibody and analogues was observed.
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 Samples    Serum
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of Lpro elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Lpro purchase    MBS704455 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Foot and Mouth Disease Virus (FMDV) antibody (Lpro) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Lpro. The ELISA analytical biochemical technique of the MBS704455 kit is based on Lpro antibody-Lpro antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lpro antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lpro. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Lpro elisa kit

   Principle of the assay: The microtiter plate provided in this kit has been pre-coated with FMDV special antigen. Controls or samples are pipetted into the wells and any FMDV antibody present is bound by the immobilized antigen. After washing, the Horseradish Peroxidase (HRP)-conjugated FMDV antibody is added to the wells. The reaction between HRP-conjugated FMDV antibody and immobilized antigen is blocked by the bounded FMDV antibody in the controls or samples. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in opposite to the amount of bovine FMDV antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS704455
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NCBI/Uniprot data below describe general gene information for Lpro. It may not necessarily be applicable to this product.
 NCBI GI #    210548
 NCBI Accession #    AAA42670.1 [Other Products]
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 UniProt Primary Accession #    P03308 [Other Products]
 UniProt Secondary Accession #    P03312; Q65038; Q65039; Q65040; Q65041; Q65042; Q65043; Q65044; Q65045; Q65046; Q65047 [Other Products]
 Molecular Weight    255,998 Da [Similar Products]
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 NCBI Official Full Name    FMDV polyprotein, partial
 UniProt Protein Name    Genome polyprotein
 UniProt Synonym Protein Names   
VP4-VP2
 UniProt Gene Name    Lpro [Similar Products]
 UniProt Synonym Gene Names    P2A; P2B; P2C; P3A; P3B-1; P3B-2; P3B-3; P3C; P3D-POL [Similar Products]
 UniProt Entry Name    POLG_FMDVA
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 UniProt Comments for Lpro    Function: The leader protease autocatalytically cleaves itself from the polyprotein at the L/VP0 junction. It cleaves the host translation initiation factors EIF4G1 and EIF4G3, in order to shut down the capped cellular mRNA transcription

Catalytic activity: Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Lpro elisa kitPathways associated with Lpro elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with Lpro and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with Lpro elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with Lpro and Bone
 Connective Tissue Antibodies  >4 publications with Lpro and Connective Tissue
 Heart Antibodies  >3 publications with Lpro and Heart
 Muscle Antibodies  >3 publications with Lpro and Muscle
 Brain Antibodies  >3 publications with Lpro and Brain
 Vascular Antibodies  >2 publications with Lpro and Vascular
 Embryonic Tissue Antibodies  >2 publications with Lpro and Embryonic Tissue
 Ear Antibodies  >1 publications with Lpro and Ear
 Kidney Antibodies  >1 publications with Lpro and Kidney
 Umbilical Cord Antibodies  >1 publications with Lpro and Umbilical Cord
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