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DHH elisa kit :: Bovine Hedgehog Homolog, Desert ELISA Kit

Scan QR to view Datasheet Catalog #    MBS060594 DHH elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hedgehog Homolog, Desert (DHH), ELISA Kit

 Also Known As   

Bovine Hedgehog Homolog, Desert ELISA Kit

 Product Gene Name   

DHH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    233420
 3D Structure    ModBase 3D Structure for O43323
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 Species Reactivity    Bovine
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 Specificity    No significant cross-reactivity or interference between Bovine DHH and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    31.2 n/ml - 1000 ng/ml.
 Sensitivity    5.0 ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DHH concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine DHH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DHH and DHH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DHH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DHH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DHH purchase    MBS060594 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hedgehog Homolog, Desert (DHH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 n/ml - 1000 ng/ml in biological research samples containing DHH, with an estimated sensitivity of 5.0 ng/ml. The ELISA analytical biochemical technique of the MBS060594 kit is based on DHH antibody-DHH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for DHH elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DHH concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine DHH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DHH and DHH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DHH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS060594. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DHH. It may not necessarily be applicable to this product.
 NCBI GI #    6166118
 NCBI GeneID    50846
 NCBI Accession #    O43323.1 [Other Products]
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 UniProt Primary Accession #    O43323 [Other Products]
 UniProt Secondary Accession #    Q15794 [Other Products]
 UniProt Related Accession #    O43323 [Other Products]
 Molecular Weight    43,577 Da
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 NCBI Official Full Name    Desert hedgehog protein
 NCBI Official Synonym Full Names    desert hedgehog
 NCBI Official Symbol    DHH [Similar Products]
 NCBI Official Synonym Symbols   
GDXYM; HHG-3; SRXY7
[Similar Products]
 NCBI Protein Information    desert hedgehog protein; mutant desert hedgehog; desert hedgehog homolog
 UniProt Protein Name    Desert hedgehog protein
 UniProt Synonym Protein Names   
HHG-3
 Protein Family    Desert hedgehog protein
 UniProt Gene Name    DHH [Similar Products]
 UniProt Synonym Gene Names    DHH [Similar Products]
 UniProt Entry Name    DHH_HUMAN
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 NCBI Summary for DHH    This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq, May 2010]
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 UniProt Comments for DHH    DHH: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development. Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD). PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity. Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM); also known as male- limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks. Belongs to the hedgehog family.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: extracellular space; plasma membrane

Molecular Function: peptidase activity; protein binding; zinc ion binding; patched binding; calcium ion binding

Biological Process: myelination; Leydig cell differentiation; cell-cell signaling; male sex determination; regulation of steroid biosynthetic process; proteolysis; spermatid development; response to estradiol stimulus

Disease: 46,xy Gonadal Dysgenesis, Partial, With Minifascicular Neuropathy; 46,xy Sex Reversal 7
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 Research Articles on DHH    1. Findings are unprecedented and indicate that the DHH-RHEBL1 fusion transcript is a novel recurrent feature in the changing landscape of CBFA2T3-GLIS2-positive childhood AML.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DHH elisa kitPathways associated with DHH elisa kit
 Reference Product  PubMed Publications
 PTCH1 elisa kit  >15 publications with DHH and PTCH1
 GLI3 elisa kit  >11 publications with DHH and GLI3
 PTCH2 elisa kit  >8 publications with DHH and PTCH2
 HHIP elisa kit  >7 publications with DHH and HHIP
 SOX10 elisa kit  >5 publications with DHH and SOX10
 BOC elisa kit  >2 publications with DHH and BOC
 BMP4 elisa kit  >2 publications with DHH and BMP4
 GAS1 elisa kit  >1 publications with DHH and GAS1
 CDON elisa kit  >1 publications with DHH and CDON
 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Ligand Biogenesis Pathway antibodies  Hedgehog Ligand Biogenesis Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hh Ligand Biogenesis Disease Pathway antibodies  Hh Ligand Biogenesis Disease Pathway Diagram
 Processing-defective Hh Variants Abrogate Ligand Secretion Pathway antibodies  Processing-defective Hh Variants Abrogate Ligand Secretion Pathway Diagram
 Release Of Hh-Np From The Secreting Cell Pathway antibodies  Release Of Hh-Np From The Secreting Cell Pathway Diagram
Diseases associated with DHH elisa kitOrgans/Tissues associated with DHH elisa kit
 Disease Name  Pubmed Publications
 Disorders of Sex Development Antibodies  >19 publications with DHH and Disorders of Sex Development
 Breast Neoplasms Antibodies  >9 publications with DHH and Breast Neoplasms
 Pancreatic Neoplasms Antibodies  >6 publications with DHH and Pancreatic Neoplasms
 Neoplasm Metastasis Antibodies  >6 publications with DHH and Neoplasm Metastasis
 Cell Transformation, Neoplastic Antibodies  >5 publications with DHH and Cell Transformation, Neoplastic
 Prostatic Diseases Antibodies  >4 publications with DHH and Prostatic Diseases
 Lung Diseases Antibodies  >4 publications with DHH and Lung Diseases
 Nervous System Malformations Antibodies  >4 publications with DHH and Nervous System Malformations
 46,XY Gonadal Dysgenesis, Complete or Partial, DHH-Related Antibodies  >4 publications with DHH and 46,XY Gonadal Dysgenesis, Complete or Partial, DHH-Related
 Inflammation Antibodies  >3 publications with DHH and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >43 publications with DHH and Testis
 Brain Antibodies  >20 publications with DHH and Brain
 Pancreas Antibodies  >7 publications with DHH and Pancreas
 Ganglia Antibodies  >3 publications with DHH and Ganglia
 Uterus Antibodies  >3 publications with DHH and Uterus
 Uncharacterized Tissue Antibodies  >2 publications with DHH and Uncharacterized Tissue
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