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IL4 elisa kit :: Bovine Interleukin 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS740126
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 4 (IL4), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Interleukin 4 ELISA Kit

 Product Gene Name   

IL4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of IL-4. No significant cross-reactivity or interference between IL-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich (Quantitative)
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL4 purchase    MBS740126 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 4 (IL4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL4, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS740126 kit is based on IL4 antibody-IL4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL4 elisa kit

   Intended Uses: This IL-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine IL-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: IL-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-4. Standards or samples are then added to the microtiter plate wells and IL-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-4 are added to each well to "sandwich" the IL-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-4 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL4 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of IL4 elisa kit    IL4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS740126. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL4. It may not necessarily be applicable to this product.
 NCBI GI #    55742622
 NCBI GeneID    397225
 NCBI Accession #    NP_999288.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_214123.1 [Other Products]
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 UniProt Secondary Accession #    Q29054; Q8MKE6 [Other Products]
 UniProt Related Accession #    Q04745 [Other Products]
 Molecular Weight    15,023 Da
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 NCBI Official Full Name    interleukin-4
 NCBI Official Symbol    IL4 [Similar Products]
 NCBI Protein Information    interleukin-4; IL-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
 UniProt Protein Name    Interleukin-4
 UniProt Synonym Protein Names   
B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
 Protein Family    Interleukin
 UniProt Gene Name    IL4 [Similar Products]
 UniProt Synonym Gene Names    IL-4; BSF-1 [Similar Products]
 UniProt Entry Name    IL4_PIG
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 UniProt Comments for IL4    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.
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 Research Articles on IL4    1. IL-4- and IL-10-producing invariant NKT cells inhibit the Th1 cell response, but not the Th17 cell response
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL4 elisa kitDiseases associated with IL4 elisa kit
 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >325 publications with IL4 and Inflammation
 Necrosis Antibodies  >319 publications with IL4 and Necrosis
 Hypersensitivity Antibodies  >318 publications with IL4 and Hypersensitivity
 Hypersensitivity, Immediate Antibodies  >255 publications with IL4 and Hypersensitivity, Immediate
 Asthma Antibodies  >223 publications with IL4 and Asthma
 Lung Diseases Antibodies  >206 publications with IL4 and Lung Diseases
 Respiratory Hypersensitivity Antibodies  >192 publications with IL4 and Respiratory Hypersensitivity
 Autoimmune Diseases Antibodies  >169 publications with IL4 and Autoimmune Diseases
 Liver Diseases Antibodies  >56 publications with IL4 and Liver Diseases
 Rhinitis Antibodies  >46 publications with IL4 and Rhinitis
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