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Lep elisa kit :: Bovine Lep (Leptin) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7606202
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Lep (Leptin), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Lep (Leptin) ELISA Kit

 Product Gene Name   

Lep elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS7606202 COA    COA PDF
 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Lep. No significant crossreactivity or interference between Lep and analogues was observed.
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 Samples    serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.313-20ng/ml
 Sensitivity    <0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lep were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lep were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Lep elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Lep purchase    MBS7606202 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lep (Leptin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/ml in biological research samples containing Lep, with an estimated sensitivity of <0.188ng/ml. The ELISA analytical biochemical technique of the MBS7606202 kit is based on Lep antibody-Lep antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lep antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lep. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Lep elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Lep antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Lep antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Lep amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Lep can be calculated.
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  Typical Testing Data/Standard Curve (for reference only) of Lep elisa kit    Lep elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7606202. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Lep. It may not necessarily be applicable to this product.
 NCBI GI #    4557715
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 Protein Family    Leptin
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Lep elisa kitPathways associated with Lep elisa kit
 Reference Product  PubMed Publications
 LEPR elisa kit  >138 publications with Lep and LEPR
 STAT3 elisa kit  >11 publications with Lep and STAT3
 INS elisa kit  >10 publications with Lep and INS
 GHRL elisa kit  >10 publications with Lep and GHRL
 CRP elisa kit  >5 publications with Lep and CRP
 JAK2 elisa kit  >4 publications with Lep and JAK2
 PTPN1 elisa kit  >1 publications with Lep and PTPN1
 SOCS3 elisa kit  >1 publications with Lep and SOCS3
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
Diseases associated with Lep elisa kitOrgans/Tissues associated with Lep elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >290 publications with Lep and Obesity
 Pain Antibodies  >156 publications with Lep and Pain
 Insulin Resistance Antibodies  >97 publications with Lep and Insulin Resistance
 Hyperinsulinism Antibodies  >64 publications with Lep and Hyperinsulinism
 Weight Gain Antibodies  >52 publications with Lep and Weight Gain
 Cardiovascular Diseases Antibodies  >48 publications with Lep and Cardiovascular Diseases
 Inflammation Antibodies  >33 publications with Lep and Inflammation
 Fatty Liver Antibodies  >33 publications with Lep and Fatty Liver
 Diabetes Mellitus, Type 2 Antibodies  >30 publications with Lep and Diabetes Mellitus, Type 2
 Liver Diseases Antibodies  >29 publications with Lep and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >315 publications with Lep and Blood
 Adipose Tissue Antibodies  >147 publications with Lep and Adipose Tissue
 Muscle Antibodies  >108 publications with Lep and Muscle
 Connective Tissue Antibodies  >106 publications with Lep and Connective Tissue
 Intestine Antibodies  >19 publications with Lep and Intestine
 Placenta Antibodies  >14 publications with Lep and Placenta
 Mouth Antibodies  >9 publications with Lep and Mouth
 Mammary Gland Antibodies  >3 publications with Lep and Mammary Gland
 Thymus Antibodies  >1 publications with Lep and Thymus
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