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MMP-12 elisa kit :: Bovine Matrix metalloproteinase 12 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS750829
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Matrix metalloproteinase 12 (MMP-12), ELISA Kit

★Popular Item★
 Also Known As   

Bovine Matrix metalloproteinase 12 ELISA Kit

 Product Gene Name   

MMP-12 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601046
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 Species Reactivity    Bovine
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MMP-12. No significant cross-reactivity or interference between MMP-12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-12 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MMP-12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MMP-12 purchase    MBS750829 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Matrix metalloproteinase 12 (MMP-12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP-12, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS750829 kit is based on MMP-12 antibody-MMP-12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP-12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP-12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MMP-12 elisa kit

   Intended Uses: This MMP-12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine MMP-12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: MMP-12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MMP-12 antibody and an MMP-12-HRP conjugate. The assay sample and buffer are incubated together with MMP-12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-12 concentration since MMP-12 from samples and MMP-12-HRP conjugate compete for the anti-MMP-12 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-12 from the sample, fewer sites are left to bind MMP-12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-12 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for MMP-12 elisa kit    Cancer
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 Typical Testing Data/Standard Curve (for reference only) of MMP-12 elisa kit    MMP-12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS750829. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MMP-12. It may not necessarily be applicable to this product.
 NCBI GI #    85567332
 NCBI GeneID    4321
 NCBI Accession #    AAI12302.1 [Other Products]
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 UniProt Secondary Accession #    Q2M1L9; B2R9X8; B7ZLF6 [Other Products]
 UniProt Related Accession #    P39900 [Other Products]
 Molecular Weight    54,002 Da
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 NCBI Official Full Name    Matrix metalloproteinase 12, preproprotein
 NCBI Official Synonym Full Names    matrix metallopeptidase 12 (macrophage elastase)
 NCBI Official Symbol    MMP12 [Similar Products]
 NCBI Official Synonym Symbols   
ME; HME; MME; MMP-12
[Similar Products]
 NCBI Protein Information    macrophage metalloelastase; matrix metalloproteinase 12 (macrophage elastase)
 UniProt Protein Name    Macrophage metalloelastase
 UniProt Synonym Protein Names   
Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12
 UniProt Gene Name    MMP12 [Similar Products]
 UniProt Synonym Gene Names    HME; MME; ME; hME; MMP-12 [Similar Products]
 UniProt Entry Name    MMP12_HUMAN
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 NCBI Summary for MMP-12    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MMP-12    MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; EC 3.4.24.65; Protease; Secreted

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells
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 Research Articles on MMP-12    1. In emphysema, MMP12 mRNA expression was inversely related to the diffusing capacity of the lung.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MMP-12 elisa kitPathways associated with MMP-12 elisa kit
 Reference Product  PubMed Publications
 IL13 elisa kit  >9 publications with MMP-12 and IL13
 CCR2 elisa kit  >3 publications with MMP-12 and CCR2
 ELN elisa kit  >2 publications with MMP-12 and ELN
 PLAUR elisa kit  >2 publications with MMP-12 and PLAUR
 WNT7A elisa kit  >1 publications with MMP-12 and WNT7A
 CXCR4 elisa kit  >1 publications with MMP-12 and CXCR4
 Products by Pathway  Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram
Diseases associated with MMP-12 elisa kitOrgans/Tissues associated with MMP-12 elisa kit
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >90 publications with MMP-12 and Lung Diseases
 Inflammation Antibodies  >78 publications with MMP-12 and Inflammation
 Cardiovascular Diseases Antibodies  >48 publications with MMP-12 and Cardiovascular Diseases
 Pulmonary Emphysema Antibodies  >40 publications with MMP-12 and Pulmonary Emphysema
 Disease Models, Animal Antibodies  >36 publications with MMP-12 and Disease Models, Animal
 Fibrosis Antibodies  >29 publications with MMP-12 and Fibrosis
 Necrosis Antibodies  >24 publications with MMP-12 and Necrosis
 Lung Injury Antibodies  >21 publications with MMP-12 and Lung Injury
 Neoplasm Invasiveness Antibodies  >18 publications with MMP-12 and Neoplasm Invasiveness
 Nervous System Diseases Antibodies  >18 publications with MMP-12 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >113 publications with MMP-12 and Lung
 Vascular Antibodies  >51 publications with MMP-12 and Vascular
 Connective Tissue Antibodies  >25 publications with MMP-12 and Connective Tissue
 Kidney Antibodies  >7 publications with MMP-12 and Kidney
 Intestine Antibodies  >6 publications with MMP-12 and Intestine
 Eye Antibodies  >6 publications with MMP-12 and Eye
 Stomach Antibodies  >4 publications with MMP-12 and Stomach
 Placenta Antibodies  >4 publications with MMP-12 and Placenta
 Esophagus Antibodies  >2 publications with MMP-12 and Esophagus
 Larynx Antibodies  >2 publications with MMP-12 and Larynx
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