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MED27 elisa kit :: Bovine Mediator of RNA polymerase II transcription subunit 27 (MED27) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7209134 MED27 elisa kit
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mediator of RNA polymerase II transcription subunit 27 (MED27), ELISA Kit

 Also Known As   

Bovine Mediator of RNA polymerase II transcription subunit 27 (MED27) ELISA Kit

 Product Gene Name   

MED27 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    605044
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 Species Reactivity    Bovine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MED27 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MED27 purchase    MBS7209134 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mediator of RNA polymerase II transcription subunit 27 (MED27) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MED27. The ELISA analytical biochemical technique of the MBS7209134 kit is based on MED27 antibody-MED27 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MED27 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MED27. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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 Product Categories/Family for MED27 elisa kit    Epigenetics and Nuclear Signaling
NCBI/Uniprot data below describe general gene information for MED27. It may not necessarily be applicable to this product.
 NCBI GI #    359751479
 NCBI GeneID    9442
 NCBI Accession #    NP_001240810.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001253881.1 [Other Products]
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 UniProt Secondary Accession #    O95401; Q4F964; Q5VTA4; Q5VTA5; Q9BU57; Q9NYR4; V9GYV9 [Other Products]
 UniProt Related Accession #    Q6P2C8 [Other Products]
 Molecular Weight    14,826 Da
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 NCBI Official Full Name    mediator of RNA polymerase II transcription subunit 27 isoform 2
 NCBI Official Synonym Full Names    mediator complex subunit 27
 NCBI Official Symbol    MED27 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    mediator of RNA polymerase II transcription subunit 27; CRSP complex subunit 8; p37 TRAP/SMCC/PC2 subunit; transcriptional coactivator CRSP34; cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa
 UniProt Protein Name    Mediator of RNA polymerase II transcription subunit 27
 UniProt Synonym Protein Names   
Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27; P37 TRAP/SMCC/PC2 subunit; Transcriptional coactivator CRSP34
 Protein Family    Mediator of RNA polymerase II transcription
 UniProt Gene Name    MED27 [Similar Products]
 UniProt Synonym Gene Names    CRSP34; CRSP8; CRSP complex subunit 8 [Similar Products]
 UniProt Entry Name    MED27_HUMAN
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 NCBI Summary for MED27    The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
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 UniProt Comments for MED27    MED27: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 27 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; Srb-mediator complex; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; stem cell maintenance; gene expression
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MED27 elisa kitPathways associated with MED27 elisa kit
 Reference Product  PubMed Publications
 MED26 elisa kit  >2 publications with MED27 and MED26
 MED11 elisa kit  >2 publications with MED27 and MED11
 MED23 elisa kit  >1 publications with MED27 and MED23
 MED17 elisa kit  >1 publications with MED27 and MED17
 MED30 elisa kit  >1 publications with MED27 and MED30
 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies  Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
 Thyroid Hormone Signaling Pathway antibodies  Thyroid Hormone Signaling Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram
Organs/Tissues associated with MED27 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1 publications with MED27 and Eye
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