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PTHRP elisa kit :: Bovine Parathyroid Hormone Related Protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS737464
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Parathyroid Hormone Related Protein (PTHRP), ELISA Kit

 Also Known As   

Bovine Parathyroid Hormone Related Protein ELISA Kit

 Product Gene Name   

PTHRP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS737464 COA    COA PDF
 Species Reactivity    Bovine
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This PTHRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PTHRP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PTHRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PTHRP purchase    MBS737464 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Parathyroid Hormone Related Protein (PTHRP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTHRP, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS737464 kit is based on PTHRP antibody-PTHRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTHRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTHRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PTHRP elisa kit

   Principle of the Assay: PTHRP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTHRP antibody and an PTHRP-HRP conjugate. The assay sample and buffer are incubated together with PTHRP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTHRP concentration since PTHRP from samples and PTHRP-HRP conjugate compete for the anti-PTHRP antibody binding site. Since the number of sites is limited, as more sites are occupied by PTHRP from the sample, fewer sites are left to bind PTHRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTHRP concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PTHRP elisa kit    PTHRP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS737464. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PTHRP. It may not necessarily be applicable to this product.
 NCBI GI #    6981438
 NCBI GeneID    24695
 NCBI Accession #    NP_036768.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012636.1 [Other Products]
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 UniProt Related Accession #    P13085 [Other Products]
 Molecular Weight    20,204 Da
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 NCBI Official Full Name    parathyroid hormone-related protein preproprotein
 NCBI Official Synonym Full Names    parathyroid hormone-like hormone
 NCBI Official Symbol    Pthlh [Similar Products]
 NCBI Official Synonym Symbols   
PLP
[Similar Products]
 NCBI Protein Information    parathyroid hormone-related protein; PTHrP; PTH-rP; parathyroid-like peptide; parathyroid hormone-like peptide; parathyroid hormone-like protein
 UniProt Protein Name    Parathyroid hormone-related protein
 UniProt Synonym Protein Names   
Parathyroid hormone-like protein; PLP
 UniProt Gene Name    Pthlh [Similar Products]
 UniProt Synonym Gene Names    Pthrp; PTH-rP; PTHrP; PLP [Similar Products]
 UniProt Entry Name    PTHR_RAT
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 NCBI Summary for PTHRP    induces vasodilation; may play a neuroprotective role in response to ischemic brain injury [RGD, Feb 2006]
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 UniProt Comments for PTHRP    PTHrP: a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Three alternatively spliced isoforms have been described.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; extracellular region; intracellular; nucleus

Molecular Function: peptide hormone receptor binding; hormone activity

Biological Process: negative regulation of transcription factor activity; negative regulation of chondrocyte differentiation; G-protein signaling, adenylate cyclase activating pathway; bone mineralization; osteoblast development; epithelial cell differentiation; regulation of gene expression; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; protein processing; regulation of chondrocyte differentiation; surfactant homeostasis; skeletal development; alveolus development; endoderm development; endochondral ossification
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 Research Articles on PTHRP    1. Pthlh is a promising candidate modifier gene in the development and progression of rat tongue cancers.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PTHRP elisa kitPathways associated with PTHRP elisa kit
 Reference Product  PubMed Publications
 Pth elisa kit  >445 publications with PTHRP and Pth
 Ihh elisa kit  >21 publications with PTHRP and Ihh
 Pthr1 elisa kit  >8 publications with PTHRP and Pthr1
 Shh elisa kit  >4 publications with PTHRP and Shh
 Il6 elisa kit  >3 publications with PTHRP and Il6
 Vegfa elisa kit  >3 publications with PTHRP and Vegfa
 Pth2r elisa kit  >3 publications with PTHRP and Pth2r
 Igf1 elisa kit  >1 publications with PTHRP and Igf1
 Products by Pathway  Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
Diseases associated with PTHRP elisa kitOrgans/Tissues associated with PTHRP elisa kit
 Disease Name  Pubmed Publications
 Hypercalcemia Antibodies  >613 publications with PTHRP and Hypercalcemia
 Bone Resorption Antibodies  >139 publications with PTHRP and Bone Resorption
 Breast Neoplasms Antibodies  >125 publications with PTHRP and Breast Neoplasms
 Lung Neoplasms Antibodies  >104 publications with PTHRP and Lung Neoplasms
 Lung Diseases Antibodies  >89 publications with PTHRP and Lung Diseases
 Kidney Diseases Antibodies  >67 publications with PTHRP and Kidney Diseases
 Necrosis Antibodies  >48 publications with PTHRP and Necrosis
 Osteolysis Antibodies  >42 publications with PTHRP and Osteolysis
 Skin Neoplasms Antibodies  >39 publications with PTHRP and Skin Neoplasms
 Neoplasms, Experimental Antibodies  >39 publications with PTHRP and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >43 publications with PTHRP and Prostate
 Brain Antibodies  >28 publications with PTHRP and Brain
 Ovary Antibodies  >15 publications with PTHRP and Ovary
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