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| Product Name
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Procalcitonin (PCT), ELISA Kit
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| Also Known As
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Bovine Procalcitonin ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Manual Insert
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| Species Reactivity
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Bovine
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of PCT. No significant cross-reactivity or interference between PCT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCT and all the analogues, therefore, cross reaction may still exist in some cases.
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| Samples
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Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
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| Assay Type
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Competitive (Quantitative)
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| Sensitivity
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0.1 mug/mL.
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Sample Preparation
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We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
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| Product Note
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Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
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| Other Notes
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Small volumes of PCT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for PCT purchase
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MBS742222 is a ready-to-use microwell, strip plate Competitive (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Procalcitonin (PCT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PCT, with an estimated sensitivity of 0.1 mug/mL. The ELISA analytical biochemical technique of the MBS742222 kit is based on PCT antibody-PCT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PCT elisa kit
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Intended Uses: This PCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PCT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: PCT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PCT antibody and an PCT-HRP conjugate. The assay sample and buffer are incubated together with PCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCT concentration since PCT from samples and PCT-HRP conjugate compete for the anti-PCT antibody binding site. Since the number of sites is limited, as more sites are occupied by PCT from the sample, fewer sites are left to bind PCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCT concentration in each sample is interpolated from this standard curve.
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| Product Categories/Family for PCT elisa kit
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Cardiovascular
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| Typical Testing Data/Standard Curve (for reference only) of PCT elisa kit
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| Sample Manual Insert of MBS742222 |
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| NCBI/Uniprot data below describe general gene information for PCT. It may not necessarily be applicable to this product. |
| NCBI GI #
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339502384
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| NCBI GeneID
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10949209
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| NCBI Accession #
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YP_004689804.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NC_015730.1
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[Other Products]
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| Molecular Weight
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69,733 Da
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| NCBI Official Full Name
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propionate CoA-transferase Pct
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| NCBI Official Symbol
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pct
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[Similar Products]
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| NCBI Protein Information
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propionate CoA-transferase Pct
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| UniProt Protein Name
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Propionate CoA-transferase Pct
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| UniProt Synonym Protein Names
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Propionate CoA-transferase Pct
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| UniProt Gene Name
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pct
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[Similar Products]
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| UniProt Synonym Gene Names
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RLO149_c008140
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[Similar Products]
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| UniProt Entry Name
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F7ZLI1_ROSLO
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Pathways associated with PCT elisa kit |
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ELISA Kit 
